Isolasi Dan Identifikasi Bakteri Pendegradasi Hidrokarbon Yang Berasal Dari Tanah Tercemar Minyak Bumi

Sopiah, Nida; Oktaviani, Avi; Sulistia, Susi; Suciati, Fuji; Aviantara, Dwindrata B

doi:10.29122/jtl.v12i3.1238

Cited by 4 publications

(5 citation statements)

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“…Bacterial activation was carried out to get active bacteria. It was due to the condition of bacteria which was inactive and the use of inactive bacteria condition was less optimal to degrade petroleum hydrocarbon compounds (Sopiah et al, 2016). The Bacterial Morphology characteristics are presented in Table 2.…”

Section: Bacterial Morphologymentioning

confidence: 99%

Viability Test of Hydrocarbonoclastic Bacteria Consortium Entrapted on Sawdust Material

et al. 2021

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Oil spills in the sea are generally prevented in various ways, one of them is the bioaugmentation method through bacterial entrapment on sawdust carriers. The entrapment of bacteria is one of the bacterial immobilization techniques. The potential of bacterial consortium on sawdust is still not widely used, especially in long-term storage. The purpose of this study was to obtain the viability of hydrocarbonoclastic bacterial consortium on sawdust after 0, 7, and 41 days of storage. The bacterial consortium consists of Bacillus aquimaris, B. megaterium, B. pumilus, Halobacillus trueperi, and Rhodobacteraceae bacterium. The samples were tested by culturing the immobilized bacterial consortium of 0, 7, and 41 days on falcon tubes containing crude oil and seawater. The viability of the immobilized bacterial consortium was tested for 28 days (days 0, 7, 14, 21, and 28) by enriched the bacterial populations periodically. The viability test was also supported by the measurement of environmental parameters on the 1 st day and the 28 th day such as DO, pH, salinity and, temperature. The results show that bacterial consortium in all storage periods was viable on sawdust carriers which related to the high number of bacterial populations on the 28 th day. The most viable bacterial consortium on sawdust was the 0-day storage (9.59 x 10 8 CFU/ml) which was indicated by the increases phase at the end of the day. It was proved that the bacteria are still productive and could degrade petroleum hydrocarbons.

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Section: Bacterial Morphologymentioning

confidence: 99%

Viability Test of Hydrocarbonoclastic Bacteria Consortium Entrapted on Sawdust Material

et al. 2021

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“…Genus Pseudomonas diketahui menghasilkan produksi dari biosurfaktan tipe rhamnolipid (Nilanjana dan Preethy, 2011). Menurut Sopiah et al (2011), Pseudomonas mampu mendegradasi senyawa Poly Aromatic Hydrocarbon (PAH) mampu memanfaatkan naftalen, phenantren dan BTEX sebagai sumber energi.…”

Section: B Genus Pseudomonasunclassified

Identifikasi Bakteri yang Berpotensi Mendegradasi Hidrokarbon dari Substrat Mangrove dengan Tekstur Berpasir, Berlumpur, dan Tanah Liat

Irene

Dirgayusa

Puspitha

2020

J. Mar. Aquat. Sci.

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Hydrocarbon compounds are commonly found in petroleum and gas, plastics, paraffins, and bitumen. Although hydrocarbons are the constituent compounds of the materials used by humans, some hydrocarbon compounds have adverse effects on the environment and humans. One alternative to solve this problem is by using Mangrove substrate in Ngurah Rai Forest Park, Bali which is the habitat of several species of bacteria that interfere with degrading hydrocarbons. This study aims to identify the bacteria that interfere with hydroxon, through macroscopic observation, microscopic and biochemical tests. The results of this study showed six isolate bacteria that interfere with degrading hydrocarbon compounds. Six isolates were from the genus Alcaligenes of sandy mangroves, Pseudomonas and Bacillus genus from muddy mangrove soils, and two genera of bacteria from clay substrate namely Alcaligenes and Bacillus. The disturbing bacterial proposals underlying the highest hydrocarbon were found on clay with a value of 58.51% and the lowest on sandy substrates, with a discount value of 0%. The highest value on the clay substrate can cause the clay substrate to bind hydrocarbons, air, nutrients, and oxygen higher than sandy and muddy soils. The lowest value on sandy substrate can lead to low bacterial capability and it takes a long time for bacteria to degrade hydrocarbons.

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“…Pada bakteri Gram negatif, setelah ditetesi larutan mordant (iodine) dan didecolorize menggunakan alkohol, kristal violet ikut terbilas. Penambahan counterstain (safranin) menyebabkan dinding sel terlihat berwana merah (Sopiah et al, 2011). Berdasarkan pewarnaan gram, didapati bahwa 12 jenis bakteri gram positif dan sisanya 6 jenis bakteri gram negatif.…”

Section: Hasil Dan Pembahasanunclassified

Identification of Water Bacteria from Nickel Post Mining in East Halmahera

Margaretta,

Iwanuddin,

Yermias

et al. 2018

wasian

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Nickel mining impact in heavy metal pollution on both soil and water. A method that is widely applied to reduce heavy metal contamination is bioremediation. Selection of bacteria that have potential reduction of heavy metal contamination in soil is very important in bioremediation process. The first step to select the potential bacteria is identification of samples. The purpose of this study is to identify existings bacteria in pond after nickel mining area PT. Antam, East Halmahera. Methods used in this research are isolation and identification of bacteria by conventional methods includes morphological, physiologica,l and biochemical test. Identification using Bergey’s Manual Determinative. The results showed there are 6 genera types of bacteria there are Bacillus, Esherichia, Enterococcus, Pseudomonas, Staphylococcus, dan Klebsiella, with 18 species in water of nickel post mining. Morphologically it is dominated by genus Bacillus as 50 % of the species. The identification of bacteria proved an existence of indigenous bacteria which is resistant to heavy metal stress.

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Isolasi Dan Identifikasi Bakteri Pendegradasi Hidrokarbon Yang Berasal Dari Tanah Tercemar Minyak Bumi

Cited by 4 publications

References 0 publications

Viability Test of Hydrocarbonoclastic Bacteria Consortium Entrapted on Sawdust Material

Viability Test of Hydrocarbonoclastic Bacteria Consortium Entrapted on Sawdust Material

Identifikasi Bakteri yang Berpotensi Mendegradasi Hidrokarbon dari Substrat Mangrove dengan Tekstur Berpasir, Berlumpur, dan Tanah Liat

Identification of Water Bacteria from Nickel Post Mining in East Halmahera

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