Isolasi, Identifikasi, dan Serotyping Avibacterium paragallinarum dari Ayam Petelur Komersial yang Menunjukkan Gejala Snot

Abstract

Infectious coryza (snot) is one of acute respiratory disease in breeders, layers, and broilers caused by Avibacterium paragallinarum (Av. paragallinarum). This disease is very harmful because of its cause decrease in egg production and high morbidity. Snot incident in Indonesia still is reported. Vaccination is one of the best preventive measures, but reports about Av. paragallinarum serotype at the field so lack so that the correspondence between serotype Av. paragallinarum in the field to those used for vaccination is unknown. Av. paragallinarum has strains with different antigenicity and until now known three serotypes there are serotypes A, B and C. Serotypes A and C are pathogenic then serotypes B. The purpose of this study was to isolate, identify and to determine the serotype of Av. paragallinarum from the commercial layer that showing symptoms of snot. Samples were taken from layer which showed symptoms of snot (nasal foul smelling exudate, infra-orbital and wattle swelling, conjunctivitis, and snoring) from some layer farms. Samples were cultured on chocolate agar and then incubated in a candle jar at 37 ᴼC for 18-24 hours. Bacteria colony and cell morphology were observed and performed biochemical tests (catalase, oxidase, urease, indole, and fermentation of carbohydrates) in suspected colonies. Serotypes test was conducted using plate agglutination test (PAT). This study revealed 4 isolates Av. paragallinarum with 2 isolates are serotype B and 2 other are serotype C.

Klasifikasi Ayam Petelur Menggunakan Artificial Neural Network dan Decision Tree

“…Berdasarkan dalam paparan singkat tersebut, penelitian pada ayam petelur sudah pernah dilakukan dengan Sistem klasifikasi otomatis ayam petelur siap potong. Dalam penelitian tersebut bertujuan untuk menyortir ayam petelur mana yang bisa dipotong dan mana yang masih bertelur [9] [10]. Hasil dalam pengujian tersebut menunjukkan 45% bobot ayam dari 320 data yang di uji.…”

The Influence of Holistic Feed on Performance of Super Native Chick

“…In general, coryza infection causes high morbidity but low mortality (El-Sawah et al, 2012). Tangkonda et al (2019) states that coryza can be diagnosed based on clinical symptoms and pathological changes. The final diagnosis can be made by isolation and identification of bacteria at an acute stage between 1-7 days after infection.…”

“…This is reinforced by Blackall and Matsumoto (2003), indicating that clinical symptoms exhibited by livestock with coryza infection experience swelling of the head, runny nose, conjunctivitis, and decreased egg production. However, further research from Tangkonda (2019) states that not all chicks with these clinical symptoms are infected with Avibacterium paragallinarum. Preventative measures include maintaining the cleanliness of the cage to reduce germs.…”

Resistansi Antibiotik Bakteri dari Ulas Kloaka Burung Puyuh Sehat

“…Hasil penelitian sebelumnya resistansi dari Avibacterium(A.) paragallinarum penyebab Snot yang diisolasi dari ayam petelur yang menunjukkan gejala Snot adalah 75% resistan terhadap enrofloksasin, 50% resistan terhadap eritromisin, 25% resistan terhadap gentamisin, 100% resistan terhadap sulfamethoxazolentrin, dan tidak ada resistansi terhadap campuran amoksisilin dan clavulanic acid (Tangkonda et al, 2019).…”