Isolated tumor endothelial cells maintain specific character during long-term culture

Matsuda, Kohei; Ohga, Noritaka; Hida, Kyoko; Muraki, Chikara; Tsuchiya, Kunihiko; Kurosu, Takuro; Akino, Tomoshige; Shih, Shou‐Ching; Totsuka, Yasunori; Klagsbrun, Michael; Shindoh, Masanobu

doi:10.1016/j.bbrc.2010.03.089

Cited by 119 publications

(156 citation statements)

References 24 publications

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“…TECs were isolated, as described previously [17][18][19][20][21][22]. In a typical procedure, the TECs were isolated from OS-RC-2 tumors and dermal tissue in tumor-bearing mice using a magnetic cell sorting system (MACS; Milteny Biotec, Tokyo, Japan).…”

Section: Isolation Of Mouse Tumor Endothelial Cells (Tecs)mentioning

confidence: 99%

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Size-controlled, dual-ligand modified liposomes that target the tumor vasculature show promise for use in drug-resistant cancer therapy

Takara

Hatakeyama

Kibria

et al. 2012

Journal of Controlled Release

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Section: Isolation Of Mouse Tumor Endothelial Cells (Tecs)mentioning

confidence: 99%

“…TECs from OS-RC-2 tumor tissue were successfully collected, as described previously [17][18][19][20][21][22]. As shown in Fig.…”

Section: Comparison Of Cytotoxicity In Tumor Cell and Tumor Endothelimentioning

confidence: 99%

Size-controlled, dual-ligand modified liposomes that target the tumor vasculature show promise for use in drug-resistant cancer therapy

Takara

Hatakeyama

Kibria

et al. 2012

Journal of Controlled Release

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“…It is assumed that a single tumor endothelial cell can support many tumor cells. [19][20][21][22][23] Thus, to develop an anti-angiogenic therapy, for targeting endothelial cells might be a much more effective strategy than targeting the actual tumor cells themselves. In order to achieve vascular targeting, our strategy was to con- struct an active targeting liposomal nano-carrier system.…”

Section: Discussionmentioning

confidence: 99%

“…Endothelial cells were isolated as previously described. [19][20][21][22][23] Briefly, normal skin endothelial cells (skin-ECs) were isolated from the dermis as controls. mTECs were isolated by magnetic bead cell sorting using an IMag cell separating system (BD Biosciences, San Jose, CA, U.S.A.).…”

Section: Isolation Of Mouse Tumor Endothelial Cells (Mtecs)mentioning

confidence: 99%

Construction of an Aptamer Modified Liposomal System Targeted to Tumor Endothelial Cells

Ara

Matsuda

Hyodo

et al. 2014

Biological & Pharmaceutical Bulletin

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We describe herein the development of a high affinity and specific DNA aptamer as a new ligand for use in liposomal nanoparticles to target cultured mouse tumor endothelial cells (mTECs). Active targeted nanotechnology based drug delivery systems are currently of great interest, due to their potential for reducing side effects and facilitating the delivery of cytotoxic drugs or genes in a site specific manner. In this study, we report on a promising aptamer candidate AraHH036 that shows selective binding towards mTECs. The aptamer does not bind to normal cells, normal endothelial cells or tumor cells. Therefore, we synthesized an aptamer-polyethylene glycol (PEG) lipid conjugate and prepared aptamer based liposomes (ALPs) by the standard lipid hydration method. First, we quantified the higher capacity of ALPs to internalize into mTECs by incubating ALPs containing 1 mol%, 5 mol% and 10 mol% aptamer of total lipids and compared the results to those for unmodified PEGylated liposomes (PLPs). A confocal laser scanning microscope (CLSM) uptake study indicated that the ALPs were taken up more efficiently than PLPs. The measured K d value of the ALPs was 142 nM. An intracellular trafficking study confirmed that most of the rhodamine labeled ALPs were taken up and co-localized with the green lysotracker, thus confirming that they were located in lysosomes. Finally, using an aptamer based proteomics approach, the molecular target protein of the aptamer was identified as heat shock protein 70 (HSP70). The results suggest that these ALPs offer promise as a new carrier molecule for delivering anti-angiogenesis drugs to tumor vasculature.Key words aptamer based liposome; targeted drug delivery; tumor endothelial cell; heat shock protein 70 (HSP70); cell-based systematic evolution of ligands by exponential enrichment Inhibiting angiogenesis is a promising strategy for the treatment of cancer and other disorders related to metastasis.

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“…Tumor EC differ from normal EC in phenotype, gene expression profile, as well as drug response (van Beijnum and Griffioen, 2005;Hida et al, 2013). It was recently shown that tumor EC from tumors with different metastatic capacity also differ in their angiogenic capacity (Matsuda et al, 2010;Ohga et al, 2012). This heterogeneity might be a mere consequence of local tumor growth characteristics and tumor cell-endothelial cell cross-talk through physical interactions and soluble mediators, which may impact therapeutic efficacy.…”

Section: F Emerging Mechanisms Of Resistancementioning

confidence: 99%

The Great Escape; the Hallmarks of Resistance to Antiangiogenic Therapy

et al. 2015

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Isolated tumor endothelial cells maintain specific character during long-term culture

Cited by 119 publications

References 24 publications

Size-controlled, dual-ligand modified liposomes that target the tumor vasculature show promise for use in drug-resistant cancer therapy

Size-controlled, dual-ligand modified liposomes that target the tumor vasculature show promise for use in drug-resistant cancer therapy

Construction of an Aptamer Modified Liposomal System Targeted to Tumor Endothelial Cells

The Great Escape; the Hallmarks of Resistance to Antiangiogenic Therapy

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