Isolates of Microbotryum violaceum from North American host species are phylogenetically distinct from their European host-derived counterparts

Freeman, Angela B; Duong, Kevin; Shi, Tieliu; Hughes, Carolyn F.; Perlin, Michael H.

doi:10.1016/s1055-7903(02)00003-9

Cited by 31 publications

(35 citation statements)

References 24 publications

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“…Assuming a random sampling of the genome, the probability of repeatedly obtaining the same locus by chance alone is small, roughly equal to the percent coverage of the sex chromosome (3 to 4%) and the autosomes (0.4%), respectively, for sequences from these sources. Additionally, by excluding rDNA and tubulin genes that have been used for phylogeny reconstruction (Perlin et al, 1997;Freeman et al, 2002), only 56 DNA sequences from M. violaceum (averaging 356 bp in length) had been deposited by other researchers into the NCBI databases. Ten of the cloned fragments from the current study matched one or more of these previously deposited sequences, further illustrating the high level of redundancy within the M. violaceum genome.…”

Section: Discussionmentioning

confidence: 99%

Repetitive DNA in the automictic fungus Microbotryum violaceum

Hood

2005

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The small genomes of fungi are expected to have little repetitive content other than rDNA genes. Moreover, among asexual or highly selfing lineages, the diversity of repetitive elements is also expected to be very low. However, in the automictic fungus Microbotryum violaceum, a very large proportion of random DNA fragments from the autosomes and the fungal sex chromosomes are repetitive in nature, either as retrotransposon or helicase sequences. Among the retrotransposon sequences, examples were found from each major kind of elements, including copia, gypsy, and non-LTR sequences. The most numerous were copialike elements, which are believed to be rare in fungi, particularly among basidiomycetes. The many helicase sequences appear to belong to the recently discovered Helitron type of transposable elements. Also, sequences that could not be identified as a known type of gene were also very repetitive within the database of random fragments from M. violaceum. The differentiated pair of fungal sex chromosomes and suppression of recombination may be the major forces determining the highly repetitive content in the small genome of M. violaceum.

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Section: Discussionmentioning

confidence: 99%

Repetitive DNA in the automictic fungus Microbotryum violaceum

Hood

2005

Genetica

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“…For molecular phylogenetic analyses the following sequences from GenBank were additionally used (Freeman et al 2002;Lutz et al 2005Lutz et al , 2008Sloan et al 2008; Microbotryum violaceo-irregulare AY588104, DQ366875;…”

Section: Phylogenetic Analysesmentioning

confidence: 99%

Microbotryum heliospermae, a new anther smut fungus parasitic on Heliosperma pusillum in the mountains of the European Alpine System

et al. 2012

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“…It includes all anther smuts of Caryophyllaceae as well as a few plants outside Caryophyllaceae (72) and forms a monophyletic group (5,36,65,79). Multiple lines of evidence indicating that M. violaceum sensu lato is a complex of distinct species include variation in spore morphology and color, large genetic distances within Microbotryum violaceum sensu lato compared to the distances between and within other Microbotryum species (65), congruence among different gene phylogenies (72), and the existence of postmating reproductive isolation (73).…”

Section: Taxonomy Of Microbotryum Violaceummentioning

confidence: 99%