Isolation and characterisation of phenol-degrading Pseudomonas aeruginosa MTCC 4996

Abstract

A novel indigenous Pseudomonas aeruginosa strain (MTCC 4996) isolated from a pulp industrial effluent-contaminated site was capable of degrading phenol up to a concentration of 1,300 mg L -1 within 156 h. Complete degradation was observed at pH values ranging from 6.0 to 10.0 and temperatures from 15 to 45°C, with an optimum pH of 7.0 and optimum temperature of 37°C. At an optimum shaking speed of 100-125 rpm, 100% degradation was observed in 66 h, as compared to 84 h under static conditions. Glucose and peptone at lower concentrations enhanced phenol degradation. The rate of phenol degradation was most sensitive to added Hg. Low concentrations of Fe, Cu, Pb, Zn, and Mn stimulated and enhanced the rate of degradation.

Biodegradación de fenol en aguas tratadas de la industria petrolera para re-uso en cultivos agrícolas

“…La utilización de microorganismos degradadores de fenol está descrita ampliamente en varios estudios que muestran resultados relevantes frente a este contaminante (Geng et al, 2006;Kotresha & Vidyasagar, 2007;Yang & Lee, 2007;Dong et al, 2008;Mohite et al, 2010). Teniendo en cuenta que el fenol es persistente en aguas de re-uso provenientes de explotación de hidrocarburos, la utilización de microorganismos es una alternativa potencial que puede ser aplicada para descontaminar este recurso, que tiende a ser escaso y por lo tanto costoso con el tiempo.…”

“…Una alternativa es la biorremediación que representa un procedimiento amigable con el medio ambiente. Varios estudios han reportado el aislamiento de microorganismos con la capacidad de degradar fenol a diferentes concentraciones, de las cuales las bacterias se han estudiado ampliamente (Geng Soh, Lim, & Loke, 2006;Kotresha & Vidyasagar, 2007;Yang & Lee, 2007;Dong, Hong, He, Jiang, & Li, 2008;Cordova-Rosa et al, 2009;Mohite, Jalgaonwala, Pawar, & Morankar, 2010). Estos estudios han elucidado que los microorganismos se encuentran distribuidos ampliamente en ambientes afectados con fenol, lo que permite pensar en su potencial aplicación para biorremediación de sitios con presencia de este contaminante.…”

Identification and Growth Characterization of a Novel 2,4-D (Dichlorophenoxyacetic Acid) Degrading Bacterium Maribacter Sp Amsu Isolated From Aquaculture Effluent

“…The effect of carbon sources on growth of Maribacter sp was individually determined by using different carbon sources such as glucose, sucrose, fructose, lactose and maltose supplemented in the basal medium at 0.5 % concentration each (Kotresha and Vidyasagar, 2008). Here 0.1 % culture broth was inoculated in to the culture media supplied with 0.2 % 2,4-D and growth was measured at every 6 h interval.…”

“…The effect of nitrogen source on growth of Maribacter sp was individually determined by using various nitrogen sources such as ammonium chloride, yeast extract, peptone, sodium nitrate and potassium nitrate supplemented in the basal medium at a concentration of 0.5 % each (Kotresha and Vidyasagar, 2008 …”

“…In this experiment 0.1 % culture broth was inoculated in different temperature (20,30,35,40, and 50 °C) range and growth was measured at every 6 h interval (Margesin and Schinner, 1997).…”