Isolation and characterisation of six novel mycobacteriophages and investigation of their antimicrobial potential in milk

Endersen, Lorraine; Coffey, Aidan; Neve, Horst; McAuliffe, Olivia; Ross, R. Paul; O’Mahony, Jim

doi:10.1016/j.idairyj.2012.07.010

Cited by 27 publications

(13 citation statements)

References 33 publications

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“…On the other hand, the six phages evaluated exhibited high resistance to basic pH; however, no viable particles were found at pH 12. Several authors evaluated phage viability at pH range from 2 to 12 and found similar results in coliphages (Coffey et al, 2011;Li et al, 2010), LAB phages (Mercanti et al, 2012) and mycophages (Endersen et al, 2013). Overall, the six phages evaluated seemed to be more resistant in an alkaline than in an acidic environment, in agreement with Feng, Ong, Hu, Tan, and Ng (2003) who found similar results for one (Qβ) of the two phages evaluated.…”

Section: Influence Of Ph On Viability and Activity Of Phagessupporting

confidence: 59%

Resistance of foodborne pathogen coliphages to thermal and physicochemical treatments applied in food manufacture

Tomat

Balagué

Casabonne

et al. 2015

Innovative Food Science & Emerging Technologies

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Section: Influence Of Ph On Viability and Activity Of Phagessupporting

confidence: 59%

Resistance of foodborne pathogen coliphages to thermal and physicochemical treatments applied in food manufacture

Tomat

Balagué

Casabonne

et al. 2015

Innovative Food Science & Emerging Technologies

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“…Phage biocontrol provides an effective tool for improving food safety, but it does not eliminate the need for safe food handling practices. For example, regrowth of bacteria was observed after phage treatment if the foods are stored at abuse temperatures [ 33 , 48 , 54 ]. Also, some planning is required to maintain optimal efficacy of phage biocontrol when combining bacteriophages with some other food safety interventions, such as using phages in conjunction with chemical sanitizers [ 59 ].…”

Section: Bacteriophage Preparations As Commercial Productsmentioning

confidence: 99%

Bacteriophage Applications for Food Production and Processing

Moye

Woolston

Sulakvelidze

2018

Viruses

421

335

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Foodborne illnesses remain a major cause of hospitalization and death worldwide despite many advances in food sanitation techniques and pathogen surveillance. Traditional antimicrobial methods, such as pasteurization, high pressure processing, irradiation, and chemical disinfectants are capable of reducing microbial populations in foods to varying degrees, but they also have considerable drawbacks, such as a large initial investment, potential damage to processing equipment due to their corrosive nature, and a deleterious impact on organoleptic qualities (and possibly the nutritional value) of foods. Perhaps most importantly, these decontamination strategies kill indiscriminately, including many—often beneficial—bacteria that are naturally present in foods. One promising technique that addresses several of these shortcomings is bacteriophage biocontrol, a green and natural method that uses lytic bacteriophages isolated from the environment to specifically target pathogenic bacteria and eliminate them from (or significantly reduce their levels in) foods. Since the initial conception of using bacteriophages on foods, a substantial number of research reports have described the use of bacteriophage biocontrol to target a variety of bacterial pathogens in various foods, ranging from ready-to-eat deli meats to fresh fruits and vegetables, and the number of commercially available products containing bacteriophages approved for use in food safety applications has also been steadily increasing. Though some challenges remain, bacteriophage biocontrol is increasingly recognized as an attractive modality in our arsenal of tools for safely and naturally eliminating pathogenic bacteria from foods.

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“…There are several general phage features that make mycobacteriophages suitable as therapeutic agents against drug-resistant TB [ 132 , 141 ]: (a) By nature, phages only infect and replicate inside their bacterial hosts without harming the human eukaryotic cells, and have the capacity to lyse and kill their hosts at the infection site [ 142 ]; (b) Their host range is mostly limited to Mycobacterium spp., which allows for the development of targeted therapies for specific M. tuberculosis strains with no apparent collateral damage to the human microbiota [ 143 ], representing one of the major advantages of phage therapy over the use of drugs; (c) Phages are the most abundant organisms in the biosphere (10 31 viral particles estimated for the entire phage population [ 144 , 145 ]), isolated from multiple environments through common and well-established microbiology techniques [ 91 ], and easy to characterize using molecular biology tools [ 146 ]. New mycobacteriophages have been sequenced and characterized these past few years [ 73 ], indicating the existence of a great phage diversity [ 147 ], which could lead to the discovery of novel mechanisms to disrupt the M. tuberculosis cell envelope; (d) Phages are easy to engineer to contain suitable features for phage therapy; (e) Because phages have the ability to replicate inside the pathogen using the bacterial cell machinery to generate more viral particles, less number of doses are required when compared to drug treatments [ 143 ].…”

Section: Phage Therapy To Treat Multidrug-resistant Tbmentioning

confidence: 99%

Mycobacteriophages as Potential Therapeutic Agents against Drug-Resistant Tuberculosis

Allué-Guardia

Saranathan

Chan

et al. 2021

IJMS

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The current emergence of multi-, extensively-, extremely-, and total-drug resistant strains of Mycobacterium tuberculosis poses a major health, social, and economic threat, and stresses the need to develop new therapeutic strategies. The notion of phage therapy against bacteria has been around for more than a century and, although its implementation was abandoned after the introduction of drugs, it is now making a comeback and gaining renewed interest in Western medicine as an alternative to treat drug-resistant pathogens. Mycobacteriophages are genetically diverse viruses that specifically infect mycobacterial hosts, including members of the M. tuberculosis complex. This review describes general features of mycobacteriophages and their mechanisms of killing M. tuberculosis, as well as their advantages and limitations as therapeutic and prophylactic agents against drug-resistant M. tuberculosis strains. This review also discusses the role of human lung micro-environments in shaping the availability of mycobacteriophage receptors on the M. tuberculosis cell envelope surface, the risk of potential development of bacterial resistance to mycobacteriophages, and the interactions with the mammalian host immune system. Finally, it summarizes the knowledge gaps and defines key questions to be addressed regarding the clinical application of phage therapy for the treatment of drug-resistant tuberculosis.

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Isolation and characterisation of six novel mycobacteriophages and investigation of their antimicrobial potential in milk

Cited by 27 publications

References 33 publications

Resistance of foodborne pathogen coliphages to thermal and physicochemical treatments applied in food manufacture

Resistance of foodborne pathogen coliphages to thermal and physicochemical treatments applied in food manufacture

Bacteriophage Applications for Food Production and Processing

Mycobacteriophages as Potential Therapeutic Agents against Drug-Resistant Tuberculosis

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