Isolation and characteristics of plasma DNA from blood donors and patients with systemic lupus erythematosus

Volková, Z; Solov'ev, G. Ya.; Folomeeva, O M

doi:10.1007/bf00836247

Cited by 3 publications

(3 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plasma levels of DNA were significantly higher in all patients than in the controls (Table 1), in whom it was 1.08+0.11 gg/ml, which coincides with published data [1][2][3][4]7]. In LAS patients with neurological symptoms assessed as 3-5 points (by 5-score scale), the content of DNA in the plasma was significantly higher: 3.83_+0.48 ~tg/rnl.…”

Section: Resultssupporting

confidence: 88%

“…However, these differences were insignificant. Thus, an increase in plasma DNA level in neurological patients did not depend on the severity of clinical condition, as was described for systemic lupus erythematosus or lymphoid leukemia [1][2][3][4]7]. There was a tendency to inverse relationship: plasma DNA content was higher (although insignificantly) in the patients with a favorable course of CCD.…”

Section: Resultsmentioning

confidence: 78%

See 1 more Smart Citation

Plasma DNA levels in patients with atherosclerotic involvement of the major arteries of the head and lateral amyotrophic sclerosis

et al. 1997

View full text Add to dashboard Cite

Measurements of DNA concentrations by fluorescence of the DNA--bisBenzimide complex in the plasma of normal subjects, patients with disorders of cerebral circulation of different severity caused by atherosclerotic involvement of the carotid and/or spinal arteries (after the acute stage of brain stroke), and patients with lateral amyotrophic sclerosis showed a significant increase in its content vs. the norm. Electrophoresis of DNA isolated from plasma showed that in the patients it is represented not only by long fragments of at least 20,000 bp (which is typical of normal subjects), but also by shorter fragments. Key Words: DNA; fluorometry; bisBenzimide; cerebral circulation disorders; lateral amyotrophic sclerosisThe blood-brain barrier is always involved in organic diseases of the nervous system; the degree of such involvement can vary from metabolic to mechanical. This involves the development of pathological process, including autoimmune reactions toward brainspecific proteins of neurons, myelin, and glial elements. There are no published reports about changes in the plasma level of DNA which may be caused by destruction of nervous tissue and by DNA excretion by activated lymphocytes [2] because of developing autoimmune reactions.We compared plasma DNA levels in two types of nervous system involvement associated with destruction of brain structures of different extent: ex-

show abstract

Section: Resultssupporting

confidence: 88%

Section: Resultsmentioning

confidence: 78%

Plasma DNA levels in patients with atherosclerotic involvement of the major arteries of the head and lateral amyotrophic sclerosis

et al. 1997

View full text Add to dashboard Cite

show abstract

“…Since 1966, work on autoimmune pathologies has permitted the first characterization of ecDNA [43][44][45][46], it is resistant to RNase and proteinase K [47], but can be hydrolyzed by DNase. Studies on ecDNA clearance revealed that extracellular DNases such as DNase1 and DNase1L3 could degrade ecDNA associated with a variety of structures [48][49][50][51][52].…”

Section: Clearance Mechanismsmentioning

confidence: 99%

Extracellular DNA in blood products and its potential effects on transfusion

Yang

et al. 2020

Bioscience Reports

View full text Add to dashboard Cite

Blood transfusions are sometimes necessary after a high loss of blood due to injury or surgery. Some people need regular transfusions due to medical conditions such as haemophilia or cancer. Studies have suggested that extracellular DNA including mitochondrial DNA present in the extracellular milieu of transfused blood products has biological actions that are capable of activating the innate immune systems and potentially contribute to some adverse reactions in transfusion. From the present work, it becomes increasingly clear that extracellular DNA encompassed mitochondrial DNA is far from being biologically inert in blood products. It has been demonstrated to be present in eligible blood products and thus can be transfused to blood recipients. Although the presence of extracellular DNA in human plasma was initially detected in 1948, some aspects have not been fully elucidated. In this review, we summarize the potential origins, clearance mechanisms, relevant structures, and potential role of extracellular DNA in the innate immune responses and its relationship with individual adverse reactions in transfusion.

show abstract

Dosage and characterization of circulating DNA: present usage and possible applications in systemic autoimmune disorders

Gargiulo

Morozzi

Piccini

et al. 2003

Autoimmunity Reviews

View full text Add to dashboard Cite

Isolation and characteristics of plasma DNA from blood donors and patients with systemic lupus erythematosus

Cited by 3 publications

References 9 publications

Plasma DNA levels in patients with atherosclerotic involvement of the major arteries of the head and lateral amyotrophic sclerosis

Plasma DNA levels in patients with atherosclerotic involvement of the major arteries of the head and lateral amyotrophic sclerosis

Extracellular DNA in blood products and its potential effects on transfusion

Dosage and characterization of circulating DNA: present usage and possible applications in systemic autoimmune disorders

Contact Info

Product

Resources

About