Isolation and Characterization of a Single-Stranded RNA Virus Infecting the Bloom-Forming Diatom
            <i>Chaetoceros socialis</i>

Tomaru, Yuji; Takao, Yoshimi; Suzuki, Hidekazu; Nagumo, Tamotsu; Nagasaki, Keisuke

doi:10.1128/aem.02580-08

Cited by 99 publications

(94 citation statements)

References 22 publications

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“…Previously reported centric diatom ssRNA and ssDNA viruses accumulate in their host cytoplasm and nucleus, respectively (Tomaru et al, 2009;Tomaru et al, 2011b), which agrees to the present observations. On the basis of the above results, we concluded that the particles in the host cells and in the lysates were viruses.…”

Section: Materials Methods Results and Discussionsupporting

confidence: 83%

“…They appeared similar to the VLP observed in their respective host cytoplasm and/or nuclei ( Figures 1e and 2e). The particle diameters of these viruses were within a range of those of the centric diatom viruses, 22-38 nm (Tomaru et al, 2009;Tomaru et al, 2011a).…”

Section: Materials Methods Results and Discussionmentioning

confidence: 76%

“…Cloning of TnitDNAV was unsuccessful because the host culture did not grow well in the microplates used for the cloning procedures and we, therefore, used non-cloned TnitDNAV in this study. Transmission electron microscopy observations for ultra-thin sections, negatively stained virions and viral genome analysis were performed using the method described by Tomaru et al (2009Tomaru et al ( , 2011a.…”

Section: Materials Methods Results and Discussionmentioning

confidence: 99%

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First evidence for the existence of pennate diatom viruses

et al. 2012

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Diatoms are considered the most successful and widespread group of photosynthetic eukaryotes. Their contribution to primary production is remarkably significant to the earth's ecosystems. Diatoms are composed of two orders: Centrales and Pennales. Thus far, viruses infecting centric diatom species have been isolated and characterized; however, viruses infecting pennates have not been reported. Here, we describe the first isolations and preliminary characterizations of two distinct pennate diatom viruses, AglaRNAV (31 nm in diameter, accumulates in the host cytoplasm) and TnitDNAV (35 nm in diameter, accumulates in the host nuclei) infecting Asterionellopsis glacialis and Thalassionema nitzschioides, respectively. Their genomes contain a single-stranded RNA of approximately 9.5 kb, and a closed, circular single-stranded DNA of approximately 5.5 kb harboring a partially double-stranded region, respectively. Further analysis of these viruses may elucidate many aspects of diatom host-virus relationships.

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Section: Materials Methods Results and Discussionsupporting

confidence: 83%

Section: Materials Methods Results and Discussionmentioning

confidence: 76%

Section: Materials Methods Results and Discussionmentioning

confidence: 99%

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First evidence for the existence of pennate diatom viruses

et al. 2012

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“…Frontiers in Marine Science | www.frontiersin.orgWhile some viruses are able to infect a wide diversity of hosts, including several bloom-forming phytoplankton species from diatoms to flagellates (Tai et al, 2003;Tomaru et al, 2009), others have a narrow host range (Short, 2012), resulting in the lysis of a specific phytoplankton taxon, thus changing the composition of phytoplankton populations.…”

Section: Viruses and Phytoplankton Mortalitymentioning

confidence: 99%

Bridging the Gap between Knowing and Modeling Viruses in Marine Systems—An Upcoming Frontier

Mateus

2017

Front. Mar. Sci.

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Viruses are the most abundant biological entities in the world's oceans. Their potential control on the dynamics and diversity of bacterioplankton and some phytoplankton groups, and consequent effect on the flow of energy and matter in food webs, may be argued as beyond dispute. Paradoxically, their importance seems to be persistently underestimated by marine modelers, frequently by exclusion, despite the uninterrupted volume of knowledge advanced during the past decades. Bridging the gap between knowing and modeling the role of viruses is, undoubtedly, one of the upcoming frontiers to be crossed in modeling the plankton. This paper has a two-fold objective: (1) review the knowledge on the roles of viruses in marine systems that has been put forward over the past decades, and (2) see how viruses have been incorporated into marine ecosystem models, along with the factors that are limiting their inclusion.

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“…The fact that the relationship between dissolved lipidomic structure and RNA viral reads was apparent without considering one of the potentially highest viral samples act as an accidental Monte Carlo test and strengthened our confidence in this finding. (Kimura and Tomarua, 2015;Nagasaki et al, 2004;Shirai et al, 2008;Tomaru et al, 2009;Tomaru et al, 2012;Tomaru et al, 2013).…”

Section: Shifts In the Dissolved Lipidome Across The Cruise Transect mentioning

confidence: 99%

The biogeochemistry of lipid derived infochemical signals in the ocean

Edwards¹

2016

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Isolation and Characterization of a Single-Stranded RNA Virus Infecting the Bloom-Forming Diatom Chaetoceros socialis

Cited by 99 publications

References 22 publications

First evidence for the existence of pennate diatom viruses

First evidence for the existence of pennate diatom viruses

Bridging the Gap between Knowing and Modeling Viruses in Marine Systems—An Upcoming Frontier

The biogeochemistry of lipid derived infochemical signals in the ocean

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