Isolation and characterization of a Pseudomonas aeruginosa from a virgin Brazilian Amazon region with potential to degrade atrazine

Fernandes, Ana Flávia Tonelli; Silva, Michelle Barbosa Partata da; Martins, Vinicius Vicente; Miranda, Carlos Eduardo Saraiva; Stehling, Eliana Guedes

doi:10.1007/s11356-014-3316-7

Cited by 27 publications

(9 citation statements)

References 31 publications

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“…Martin-Laurent et al (2003) showed that the atrazine-degrading community rapidly increased in the presence of atrazine and may be regarded as an opportunistic microbiota. Furthermore, the atrazine degrader Pseudomonas was isolated from Amazon Forest soil with no history of pesticide use (Fernandes et al, 2014). In contrast, Kersantè et al (2006) suggested that a treatment with atrazine did not significantly affect atzA, atzB, or atzC gene abundance in soil samples with no bioaugmentation.…”

Section: Discussionmentioning

confidence: 98%

Impact of Atrazine Exposure on the Microbial Community Structure in a Brazilian Tropical Latosol Soil

et al. 2020

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Atrazine is a triazine herbicide that is widely used to control broadleaf weeds. Its widespread use over the last 50 years has led to the potential contamination of soils, groundwater, rivers, and lakes. Its main route of complete degradation is via biological means, which is carried out by soil microbiota using a 6-step pathway. The aim of the present study was to investigate whether application of atrazine to soil changes the soil bacterial community. We used 16S rRNA gene sequencing and qPCR to elucidate the microbial community structure and assess the abundance of the atrazine degradation genes atzA, atzD, and trzN in a Brazilian soil. The results obtained showed that the relative abundance of atzA and trzN, encoding triazine-initiating metabolism in Gram-negative and -positive bacteria, respectively, increased in soil during the first weeks following the application of atrazine. In contrast, the abundance of atzD, encoding cyanuric acid amidohydrolase -the fourth step in the pathway-was not related to the atrazine treatment. Moreover, the overall soil bacterial community showed no significant changes after the application of atrazine. Despite this, we observed increases in the relative abundance of bacterial families in the 4 th and 8 th weeks following the atrazine treatment, which may have been related to higher copy numbers of atzA and trzN, in part due to the release of nitrogen from the herbicide. The present results revealed that while the application of atrazine may temporarily increase the quantities of the atzA and trzN genes in a Brazilian Red Latosol soil, it does not lead to significant and long-term changes in the bacterial community structure.

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Section: Discussionmentioning

confidence: 98%

Impact of Atrazine Exposure on the Microbial Community Structure in a Brazilian Tropical Latosol Soil

et al. 2020

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“…For isolation, an evaluation of the growth and degradation of fipronil adapted from a medium (ATZ-R) is described by Fernandes et al without a nitrogen source (NH 4 NO 3 ) being used. 42 The culture medium consisted of 1 mol L −1 KH 2 PO 4 (67.0 mL L −1 ), 0.7 mol L −1 sodium citrate (2.0 g L −1 ), 1.0 mol L −1 of CaCl 2 •2H 2 O (0.2 mL L −1 ), and salt R solution (5 mL L −1 ). For solid medium, 2% bacteriological agar was added.…”

Section: Culture Media and Reagentsmentioning

confidence: 99%

“…The R salt solution was prepared as follows: MgSO 4 •7H 2 O (80.0 g L −1 ), FeSO 4 •7H 2 O (2.0 g L −1 ), and HCl (4.0 mL L −1 ). The glucose (0.2 g L −1 ) used by Fernandes et al 42 it was not used in present medium. The pH was adjusted to 6.8 and the medium was autoclaved at 121 °C for 20 min using fipronil as the sole nitrogen source (0.6 g L −1 ) (Badische Anilin & Soda Fabrik, BASF (Ludwigshafen am Rhein, Alemanha); 92.8% purity).…”

Section: Culture Media and Reagentsmentioning

confidence: 99%

Fipronil biodegradation and metabolization by Bacillus megaterium strain E1

Prado

Pereira

Durrant³

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND: Fipronil is a broad-spectrum insecticide that is used extensively due to its effective action in pest control. However, environmental studies have shown it has high toxicity towards non-target organisms as well. In addition, the degradation of fipronil can generate even more toxic and reactive metabolites in the environment. In the present study, bioprospecting for bacteria with the potential to degrade fipronil was performed using fipronil as the sole source of nitrogen and main source of carbon. From samples of corn culture soil with a history of fipronil application, isolation was performed using the microcosm enrichment method. RESULTS: Bacteria were isolated using fipronil (0.6 g L −1 ) as the sole nitrogen source and main carbon source. After preliminary tests, isolate E1 was selected and, following sequencing of 16S rRNA, it was found that the isolate E1 was a bacterium of the Bacillaceae family, Bacillus megaterium species. Growth was evaluated by dry biomass and degradation quantified by gas chromatography-mass spectrometry (GC-MS). Strain E1 presented a 94% degradation of fipronil, as well as 91% and 96% degradation of its metabolites fipronil-sulfide and fipronil-sulfone, respectively, in 14 days, reducing its initial concentration from 0.6 g L −1 to 0.036 mg L −1 . CONCLUSION: The results showed that bioprospecting of the E1 strain, isolated from soil, showed efficiency in the biodegradation process of fipronil. This strain is a potential candidate for use in bioremediation processes in areas contaminated with fipronil and for use in further studies on the elucidation of the metabolic pathways of fipronil degradation.

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“…These concentrations might still be relevant for human and ecosystem health (Shipitalo and Owens, 2003;Jablonowski and Schäffer, 2011;Vonberg et al, 2014). This persistence is surprising, given that studies have confirmed 1) the frequent presence of bacterial strains able to completely degrade atrazine (under controlled conditions) (Udikoviç- Koliç et al, 2012;Fernandes et al, 2014;Ehrl et al, 2018b;Kundu et al, 2019); and 2) alternative photolytic degradation of atrazine in soil (Kiss et al, 2007;Kiss and Virág, 2009;López-Muñoz et al, 2011). The persistence of atrazine and other pesticides in natural environments demands a better understanding of degradation processes to improve long-term monitoring and pollution mitigation strategies (Chow et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Modeling Bioavailability Limitations of Atrazine Degradation in Soils

Rodriguez

Ingalls

Meierdierks

et al. 2021

Front. Environ. Sci.

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Pesticide persistence in soils is a widespread environmental concern in agro-ecosystems. One particularly persistent pesticide is atrazine, which continues to be found in soils and groundwater in the EU despite having been banned since 2004. A range of physical and biological barriers, such as sorption and mass-transfer into bacterial cells, might limit atrazine degradation in soils. These effects have been observed in experiments and models working with simplified systems. We build on that work by developing a biogeochemical model of the degradation process. We extended existing engineered system models by including refined representations of mass-transfer processes across the cell membrane as well as thermodynamic growth constraints. We estimated model parameters by calibration with data on atrazine degradation, metabolite (hydroxyatrazine) formation, biomass, and isotope fractionation from a set of controlled retentostat/chemostat experiments. We then produced site-specific model predictions for arable topsoil and compared them with field observations of residual atrazine concentrations. We found that the model overestimated long-term atrazine biodegradation in soils, indicating that this process is likely not limited by bioavailability or energetic constraints of microbial growth. However, sorption-limited bioavailability, could explain the long-term fate and persistence of the main degradation metabolite hydroxyatrazine. Future studies should seek alternative controls that drive the observed atrazine persistence in soil. This work helps to bridge the gap between engineered and natural systems, allowing us to use laboratory setups to gain insight into real environmental systems.

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Isolation and characterization of a Pseudomonas aeruginosa from a virgin Brazilian Amazon region with potential to degrade atrazine

Cited by 27 publications

References 31 publications

Impact of Atrazine Exposure on the Microbial Community Structure in a Brazilian Tropical Latosol Soil

Impact of Atrazine Exposure on the Microbial Community Structure in a Brazilian Tropical Latosol Soil

Fipronil biodegradation and metabolization by Bacillus megaterium strain E1

Modeling Bioavailability Limitations of Atrazine Degradation in Soils

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