Isolation and characterization of an AGAMOUS homologue from cocoa

Chaidamsari, Tetty; Samanhudi,; Sugiarti, Herti; Santoso, Djoko; Angenent, Gerco C.; Maagd, Ruud A. de

doi:10.1016/j.plantsci.2006.01.006

Cited by 19 publications

(12 citation statements)

References 23 publications

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“…MADS-box transcription factors are major regulators of development in plants. The factors act in a combinatorial manner, either as homo-or heterodimers, and control floral organ formation and identity and many other developmental processes in leaves and roots through a complex network of protein-protein and protein-DNA interactions [30][31][32].…”

Section: Resultsmentioning

confidence: 99%

<i>Hordeum vulgare</i> miežių lapų stabilių DNR baltymų kompleksų baltymų sudėties analizė

Bielskienė¹,

Bagdoniene

Labeikytė³

et al. 2009

Biologija

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Tightly bound to DNA proteins (TBP) are a protein group that remains attached to DNA with covalent or non-covalent bonds after its deproteinisation [1,2]. TBP have been found in DNA of numerous evolutionary distant species [3][4][5]. Some of these TBP proteins have been characterized in Ehrlich ascites [6-8] and yeast (Saccharomyces cerevisiae) [9] cells. The aim of this work was to characterize TBP proteins in the first leaves of barley (Hordeum vulgare) shoots by the MALDI TOF-TOF mass-spectrometry (MS) analysis. We have identified that most of these proteins (WRKY transcription factors 16 and 52, MADS-box transcription factor 26, Squamosa promoter-binding-like protein 16, Scarecrow-like protein 9, TGA4, TEOSINTE BRANCHED 1) are transcription factors playing important roles in the development and coping with either biotic or abiotic stress in plants. Various barley TBP (above transcription factors, DEMETER-like protein 2, HAC12, RAD51, Ty3-gypsy retrotransposon, protein kinases, serpins) participate in chromatin rearrangement and the regulation of gene expression.

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Section: Resultsmentioning

confidence: 99%

<i>Hordeum vulgare</i> miežių lapų stabilių DNR baltymų kompleksų baltymų sudėties analizė

Bielskienė¹,

Bagdoniene

Labeikytė³

et al. 2009

Biologija

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“…All four sequences were most closely related to plant MADS-box proteins of the AGAMOUS class, with 75-95% amino acid identity to proteins such as AGAMOUS-like protein from Cacao (Chaidamsari et al 2006), CAG1 (Perl-Treves et al 1998) and CUM10 (Kater et al 1998) from cucumber, Cit-MADS6 from mandarin (Endo et al 2006), LAG from sweetgum (Liu et al 1999) and SHATTERPROOF-like protein from peach (accession no. DQ777635).…”

Section: Molecular Cloning Of Full-length Cotton Mads-box Cdnasmentioning

confidence: 99%

Evidence for alternative splicing of MADS-box transcripts in developing cotton fibre cells

et al. 2007

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The MADS-box family of genes encodes transcription factors that have widely ranging roles in diverse aspects of plant development. In this study, four cotton MADS-box cDNA clones of the type II (MIKC) class were isolated, with phylogenetic analysis indicating that the cotton sequences are of the AGAMOUS subclass. The corresponding transcripts were detected in developing cotton fibre cells as well as in whole ovule and flower tissue, with differential expression in stems, leaves and roots. Reverse transcription PCR showed extensive alternative splicing in one of the reactions, and 11 mRNAs of different intron/exon composition and length were characterised. Sequence differences between the transcripts indicated that they could not be derived from the same pre-mRNA and that the sequenced transcript pool was derived from two distinct MADS-box genes. Several of the alternatively spliced transcripts potentially encoded proteins with altered K-domains and/or C-terminal regions and the variant proteins may have altered cellular roles. This work is the first that describes MADS-box gene expression in elongating cotton fibres and adds to a growing body of evidence for the prevalence of alternative splicing in the expression of MADS-box and other genes.

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“…RNA total diisolasi dari bantalan bunga aktif kakao menggunakan prosedur yang diuraikan oleh Chaidamsari et al, (2006) dengan sedikit modifikasi. Sebanyak 1 gram jaringan dihaluskan dengan nitrogen cair dan dihomogenkan dengan bufer ekstraksi (EDTA 20 mM pH 8,0; CTAB 2%, NaCl 2 M, Tris HCl 100 mM pH 8,0; PVP) dan 1% β-merkaptoetanol pada suhu 65°C selama satu jam dan pengocokan setiap 15 menit.…”

Section: Isolasi Rna Totalunclassified

Kloning gen LEAFY kakao dari jaringan bantalan bunga aktif Cloning of cacao LEAFY gene from the active flower cushions

CHAIDAMSARI¹,

HAYATI²,

SYARIEF³

et al. 2016

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Summary Attempts to improve productivity of cacao plantations lead us to study the molecular mechanism of flowering. In the model species RingkasanUsaha untuk meningkatkan produktivitas perkebunan kakao telah mendorong penelitian molekuler tentang mekanisme pembungaan kakao. Pada tanaman model Arabidopsis thaliana dan lainnya, LEAFY merupakan gen kunci dalam transisi meristem tunas jadi meristem bunga. Berbeda dengan sistem pada Arabidopsis, pembungaan kakao termasuk tipe cauliflorous, bunga dapat muncul dari bantalan bunga yang sama sepanjang tahun. Dalam penelitian ini homolog LFY diisolasi dari bantalan bunga aktif menggunakan RT-PCR dengan sepasang primer spesifik yang dirancang berdasarkan sekuen DNA di kedua ujung gen tersebut. Pemeriksaan gel elektroforesis menunjukkan adanya amplikon tunggal berukuran 1,2 kb. Setelah dimurnikan dari gel, amplikon dapat diklon ke dalam sel kompeten E. coli galur XL1 Blue menggunakan vektor pGEM-T Easy dengan orientasi yang sesuai dengan promoter T7 dari vektor. Analisis BLASTX sekuen DNA membuktikan bahwa amplikon tersebut adalah homolog dari gen LEAFY. Analisis penjajaran dengan menggunakan ClustalW menunjukkan bahwa gen cTcLFY tersebut memiliki homologi yang tinggi dengan gen sejenis dari tanaman keras lainnya seperti tanaman jeruk, anggur, apel dan poplar. Homologi tertinggi (daerah terkonservasi) terdapat pada ujung (terminal) C dari protein yang disandinya. *) Penulis korespondensi, +) Penulis berkontribusi sepadan

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Isolation and characterization of an AGAMOUS homologue from cocoa

Cited by 19 publications

References 23 publications

<i>Hordeum vulgare</i> miežių lapų stabilių DNR baltymų kompleksų baltymų sudėties analizė

<i>Hordeum vulgare</i> miežių lapų stabilių DNR baltymų kompleksų baltymų sudėties analizė

Evidence for alternative splicing of MADS-box transcripts in developing cotton fibre cells

Kloning gen LEAFY kakao dari jaringan bantalan bunga aktif Cloning of cacao LEAFY gene from the active flower cushions

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