Isolation and characterization of an aspartic protease able to hydrolyze and decolorize heme proteins from Aspergillus glaucus

Abstract

The results of the present study demonstrate the potential utility of Aspergillus molds as a functionally new microbial resource for industrial applications such as the bleaching of heme proteins. © 2018 Society of Chemical Industry.

Ethnic seafood products sold on the Italian market: labelling assessment and biological, chemical and physical risk characterization

“…Indeed, xerophilic fungi, such as A. glaucus and A. repens, are commonly used for ripening and fermentation of typical Asian products, e.g. Katsuobushi (dried bonito) (Takenaka, Lim, Fukami, Yokota, & Doi,Takenaka et al, 2018). A. flavus, instead, can represents a biological hazard for its potential aflatoxigenic activity(Ikutegbe & Sikoki, 2014).…”

Species-specific effects of the introduction of Aspergillus nidulans gfdB in osmophilic aspergilli

“…In a previous study by Király et al ( 2020b ), we managed to supplement A. glaucus with A. nidulans gfdB resulting in ’c gfdB strains with considerably increased t BOOH and, to a lesser extent, increased H 2 O 2 , MSB, Congo Red, and CdCl 2 tolerance. Since A. glaucus is a promising enzyme (Tao et al 2010 , 2011 ; Abrashev et al 2016 ; Li et al 2018 ; Takenaka et al 2019 ; Chen et al 2020 ) and polyketide (Cai et al 2009 , 2014 ; Sun et al 2009 ; Wu et al 2017 ) producer and bioremediation (Gajendiran and Abraham 2017 ; Wei and Zhang 2018 ; Zhou et al 2021 ) fungus, and a satisfactory stress tolerance is highly recquired for any industrial fungal strains (Bai et al 2003 ; Li et al 2011 ; Teixeira et al 2011 ; Hagiwara et al 2016 ; Deparis et al 2017 ; Steensels et al 2019 ; Brandt et al 2021 ; Yaakoub et al 2022 ), these observations raised the question if the contribution of A. nidulans gfdB to oxidative stress tolerance could be exploited in other Aspergillus spp. as well.…”

“…In these fungi, osmophily was hypothesized to be connected to the lack of the gfdB gene, encoding a putative NAD + -dependent glycerol-3-phosphate dehydrogenase enzyme in many Aspergillus spp., including the filamentous fungus model organism Aspergillus nidulans (Miskei et al 2009 ; Balázs et al 2010 ; de Vries et al 2017 ). Importantly, the xerophilic/osmophilic A. glaucus may also find its industrial fermentation applications in enzyme (Tao et al, 2010 , 2011 ; Abrashev et al 2016 ; Li et al 2018 ; Takenaka et al 2019 ) and aspergiolide A (an anticancer-polyketide) (Cai et al 2009 , 2014 ; Sun et al 2009 ; Wu et al 2017 ) production, and A. nidulans is also a well-known enzyme producer fungus and represents a potential platform for heterologous protein expression (MacCabe et al 2002 ; Kumar 2020 ; Lopes et al 2021 ; Jin et al 2022 ). It is noteworthy that A. glaucus , which has a remarkably high abiotic stress resistance, is also considered a suitable tool in saline-alkaline remediation technologies (Wei and Zhang 2018 ; Zhou et al 2021 ), in the biomineralization of the insecticide fipronil (Gajendiran and Abraham 2017 ), and in the hydrolysis of sugar cane bagasse (Tao et al 2010 ).…”

Screening, identification, and characterization of molds for brewing rice wine: Scale-up production in a bioreactor

“…The spore suspension was diluted in a gradient, and 0.1 mL of suitable diluted spore suspension was collected by suction and coated on Rose Bengal medium (glucose, 10 g; peptone, 5 g; agar, 15 g; KH 2 PO 4 , 1 g; MgSO 4 , 0.5 g; Bengal red, 0.03 g; chloramphenicol, 1.0 g; distilled water, 1,000 mL; pH 7.0; sterilized at 121°C for 15 min) and cultured at 28°C. Single colonies with detectable mold morphology were selected and inoculated onto a PDA slope (15 g/1,000 mL agar) for preservation [ 15 ].…”