Isolation and characterization of bacteria from mangrove sediment at coastal area in Pangkep South Sulawesi

Ambeng,; Zubair, Hazairin; Oka, NP; Tonggiroh, Adi

doi:10.1088/1742-6596/1341/2/022016

Cited by 7 publications

(9 citation statements)

References 11 publications

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“…Our results suggested that the NA media is suitable for counting and isolating microbes from mangrove soil and sediments. This matches well with what was found by Ambeng et al, (2019), who used nutrient agar media to grow total colony counts from mangroves. In order to explore the fungi, the Potato Dextrose Agar medium for fungi was used by Ravikumar et al, (2004), and other researchers used Czapex dox (Thupurani et al, 2016).…”

Section: Preservationsupporting

confidence: 90%

“…Despite much research in this field, the results are somewhat conflicting and confusing. For example, some use nutrient agar for the growth of total colony counts from mangrove soil (Kumar et al, 2007;Ambeng et al, 2019) while some use Tryptic Soya Agar (TSA) (Pupin and Nahas, 2014).In comparison, others preferred Zobell's marine agar (ZB agar), and synthetic seawater media (Sakhia et al, 2016;Saravanakumar et al, 2016;Schut et al, 1993). Our results suggested that the NA media is suitable for counting and isolating microbes from mangrove soil and sediments.…”

Section: Preservationmentioning

confidence: 88%

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Isolation and Abundance of Different Culturable Microbes from Mangrove Environments in Coastal Areas of Saudi Arabia

Sohaib¹,

Al-Barakah²,

Migdadi³

et al. 2022

Int.J.Curr.Microbiol.App.Sci

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Mangrove are native to unique stressful habitat and mangrove-associated microbes may be one of the reasons behind the survival of this plant in this habitat. Therefore, it is necessary to explore the microbial diversity from the unstudied mangrove ecosystem because exploration of microbial associates of mangroves may help full to screen such potential specialized microbial strains that are as unique as their habitat. Samples of 8 different locations along the Arabian Gulf and the Red Sea were collected for enumeration and isolation of microbes based on different general and selective agar mediums. The results revealed that the maximum colony counts of total (1.01 × 107 CFU mean /g dry soil), fungi (1.18 × 104 CFU mean /g dry soil), and actinomycetes (5.93 × 104 CFU mean /g dry soil) were observed in L1 (MRS soil), L44 (MRS soil), and L3 (MRS soil) respectively. While minimum colony counts of total (5.32 × 104 CFU mean/g dry soil), fungi (2.59 × 102 CFU mean/g dry soil) and actinomycetes (2.61 × 10 CFU mean/g dry soil) were observed in L47 (MS soil), L3 (MS soil), and L4 (MS soil) respectively. L3 (MRS soil), L2 (MRS soil and MS soil), L1 (MRS soil), L44 (MRS soil), and L5 (NMRS soil and NMS soil) showed the growth of all studied groups of microbes other than sulfur oxidizers, while water samples, L3 (MSW water), L2 (MSW water), L47 (MSW water), L4 (MSW water), and L1 (MSW water) showed growth of limited groups as compared to soil samples.

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Section: Preservationsupporting

confidence: 90%

Section: Preservationmentioning

confidence: 88%

Isolation and Abundance of Different Culturable Microbes from Mangrove Environments in Coastal Areas of Saudi Arabia

Sohaib¹,

Al-Barakah²,

Migdadi³

et al. 2022

Int.J.Curr.Microbiol.App.Sci

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“…Approximately 1 g of sediment and 1 mL of hot water samples were inoculated into 9 mL of each nutrient broth separately in a test tube and incubated at 55°C for 24 h. This was continued with serial dilution, spread on nutrient agar plates, and incubated at 60°C for 48 h. Bacterial colonies that grow on the agar surface were purified by stretching them to a new medium in a Petri plate and incubated for 48 h at 55°C (Ambeng et al 2019). The isolates were characterized morphologically and biochemically, including elevation, margin, color, Gram staining, catalase test, motility, triple sugar iron agar test, simmon citrate, and gelatinase.…”

Section: Bacterial Isolation and Characterizationmentioning

confidence: 99%

Molecular identification of thermophilic bacteria with antimicrobial activity isolated from hot springs in North Sumatra, Indonesia

Ginting

PISKA²,

HARMILENI³

et al. 2023

Biodiversitas

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Abstract. Ginting CN, Piska F, Harmileni, Fachrial E. 2023. Molecular identification of thermophilic bacteria with antimicrobial activity isolated from hot springs in North Sumatra, Indonesia. Biodiversitas 24: 752-758. Microorganisms such as thermophilic bacteria are promising sources of antimicrobial compounds due to their abundance, which has yet to be widely investigated. Therefore, this study aims to identify thermophilic bacteria isolated from natural hot springs, Sidebuk Debuk, in North Sumatra, Indonesia. The bacteria were isolated from sediments and hot springs water. Isolated 10 bacteria were characterized and identified based on their morphological and biochemical properties, which included gelatinase activity, motility, citrate utilization, triple sugar iron agar test, catalase test, cell shape, color, and Gram staining. Antimicrobial activity was tested using the disc diffusion method against Escherichia coli and Staphylococcus aureus. The most potential isolates were identified molecularly by amplification of 16S rRNA genes using universal primers. The sequences result were trimmed and assembled using BioEdit and submitted to BLAST to determine the homology of the bacteria. The isolate ITU9 was identified as Bacillus megaterium and exhibited the best antimicrobial activity with an inhibition zone of 9.5 mm against S. aureus during 72 h of incubation. This research showed that the thermophilic B. megaterium strain ITU9 was successfully isolated from Sidebuk Debuk hot springs and had stable antimicrobial activity for 72 h against E. coli and S. aureus.

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“…Bacteria is one kind of microorganisms, there are two groups of bacteria, they are archaebacteria and eubacteria (Sattley & Madigan, 2015), moreover, eubacteria/bacteria is more common and has various kind of their own group. These bacteria can also live in the soil as a decomposer, such as decomposer in mangrove soil (Chen et al, 2016;Sakhia et al, 2016;Wu et al, 2016;Ambeng et al, 2019). Recent study shows that bacteria live in mangrove soil to decompose mangrove litter and constantly live at there due to condusive environment to them for living.…”

Section: Microbiologymentioning

confidence: 99%

“…Recent study shows that bacteria live in mangrove soil to decompose mangrove litter and constantly live at there due to condusive environment to them for living. Some example mangrove bacteria are from genus Bacillus (Sakhia et al, 2016;Ambeng et al, 2019), Microccous, Yangia, Rhodococcus, Escherecia, Pseudotoromonas, Aerococcus, Pseudoteromonas (Sakhia et al, 2016), Staphylococcus, Vibrio, Alteromonas, Listeria, Macrococcus (Ambeng et al, 2019), Proteobacteria, Chloroflexi, Bacteriodetes, Planctomycetes (Wu et al, 2016). Those bacteria and mangrove soil can be potential learning sources for stundents.…”

Section: Microbiologymentioning

confidence: 99%

The Result of Second Field Test of Cellulose Degrading Bacteria from Mangrove Soil Microbiology Handout

Nugraheni

Hastuti

Rohman

2020

Journal of Biological Science and Education

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Learning media is one of the most important component in a learning process, both inside or outside a classroom. In order to provide proper learning for students based on the researcher's prior research, at least, three different field tests are needed. This research is a quasi-experiment, which is provide result of the second field test of cellulose degrading bacteria from mangrove soil microbiology handout both in cognitive and psychomotor ability. The research aims are to describe effect of this microbiology handout on student's cognitive achievement and gain data about inappropriate laboratory written instruction in this handout. Data gained by conducting learning activities using developed handout which were 2 times in class theory and three times of practical activity using instuctions written in the handout. In the end of class theory activity, students did a posttest to confirm their improvement of cognitive ability while their practical ability was observed using observation sheet along with their activities. Results showed that handout which being tested could improve student cognitive at average level (n gain 0,419). In contrast, several parts of laboratory written instruction need to be fixed to make better microbiology handout.

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Isolation and characterization of bacteria from mangrove sediment at coastal area in Pangkep South Sulawesi

Cited by 7 publications

References 11 publications

Isolation and Abundance of Different Culturable Microbes from Mangrove Environments in Coastal Areas of Saudi Arabia

Isolation and Abundance of Different Culturable Microbes from Mangrove Environments in Coastal Areas of Saudi Arabia

Molecular identification of thermophilic bacteria with antimicrobial activity isolated from hot springs in North Sumatra, Indonesia

The Result of Second Field Test of Cellulose Degrading Bacteria from Mangrove Soil Microbiology Handout

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