Isolation and characterization of cDNAs and genomic DNAs encoding ADP-glucose pyrophosphorylase large and small subunits from sweet potato

Zhou, Yue; Chen, Yuxiang; Xiang, Tao; Cheng, Xiaoyu; Wang, Haiyan

doi:10.1007/s00438-015-1134-3

Cited by 16 publications

(7 citation statements)

References 40 publications

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“…Especially IbAGPb3 , which was scarcely expressed in sweet potato SRs. This result is similar to the findings of a previous study that examined the expression of genes encoding AGPase subunits in sweet potato (Zhou et al, 2016), which showed that IbAGPb3 was only expressed in leaves and IbAGPb2 was expressed in sink tissues at low levels. However, our results also showed that IbAGPb3 was expressed at 95 DAP in the SRs of YS33 and XS22 (Figure 6 and Figure S3D); thus, the tissue specificity and role of this subunit remain to be determined.…”

Section: Discussionsupporting

confidence: 91%

Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato

Zhang

Tang

et al. 2017

Front. Plant Sci.

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The starch properties of the storage root (SR) affect the quality of sweet potato (Ipomoea batatas (L.) Lam.). Although numerous studies have analyzed the accumulation and properties of starch in sweet potato SRs, the transcriptomic variation associated with starch properties in SR has not been quantified. In this study, we measured the starch and sugar contents and analyzed the transcriptome profiles of SRs harvested from sweet potatoes with high, medium, and extremely low starch contents, at five developmental stages [65, 80, 95, 110, and 125 days after transplanting (DAP)]. We found that differences in both water content and starch accumulation in the dry matter affect the starch content of SRs in different sweet potato genotypes. Based on transcriptome sequencing data, we assembled 112336 unigenes, and identified several differentially expressed genes (DEGs) involved in starch and sucrose metabolism, and revealed the transcriptional regulatory network controlling starch and sucrose metabolism in sweet potato SRs. Correlation analysis between expression patterns and starch and sugar contents suggested that the sugar–starch conversion steps catalyzed by sucrose synthase (SuSy) and UDP-glucose pyrophosphorylase (UGPase) may be essential for starch accumulation in the dry matter of SRs, and IbβFRUCT2, a vacuolar acid invertase, might also be a key regulator of starch content in the SRs. Our results provide valuable resources for future investigations aimed at deciphering the molecular mechanisms determining the starch properties of sweet potato SRs.

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Section: Discussionsupporting

confidence: 91%

Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato

Zhang

Tang

et al. 2017

Front. Plant Sci.

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“…In sweetpotato, AGPase is a key enzyme controlling starch synthesis and is considered an important determinant of the sink activity of the roots (Yatomi et al 1996;Tsubone et al 2000). Many AGPase genes have been cloned and studied in sweetpotatoes (Lee et al 2000;Seo et al 2015;Zhou et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Identification of the conserved domains of ADP-Glucose Pyrophosphorylase (AGPase) protein in sweetpotato (Ipomoea batatas (L.) Lam.) and its two wild relatives

Nie

Kim

Ho-Hyun

et al. 2021

Preprint

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The conserved domains are defined as recurring units in molecular evolution, which are commonly used to interpret the molecular function and biochemical structure of proteins. The AGPase amino acid sequences of three species from the Ipomoea genus were identified to investigate their physicochemical and biochemical characteristics. The molecular weights (MW), isoelectric point (pI), instability index (II), and grand average of hydropathy (GRAVY) showed considerable differences in each plant. The aliphatic index (AI) values of sweetpotato AGPase proteins were higher in the small subunit than in the large subunit. The AGPase proteins from sweetpotato contain an LbH_G1P_AT_C domain in the C-terminal region and various domains (NTP_transferase, ADP_Glucose_PP, or Glyco_tranf_GTA) in the N-terminal region. On the other hand, most of its two relatives (I. trifida and I. triloba) only contain the NTP_transferase domain in the N-terminal region. These findings suggested that these conserved domains were species specificity and related to the subunit types of AGPase proteins. The study may enable research on the AGPase-related specific characteristics of sweetpotatoes, which do not exist in the other two species, such as starch metabolism and tuberization mechanism.

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“…Many key genes have been cloned and functionally characterized in higher plants, especially in the model plant Arabidopsis thaliana and species with starchy organs such as rice, corn, potato, and cassava (Lloyd and Kossmann, 2019;Smith and Zeeman, 2020). Zhou et al (2016) reported the comprehensive cloning of AGPase isoforms in Ipomoea batatas, including two for AGPS and four for AGPL. Similar work has been performed in Nelumbo nucifera (Cheng et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning, characterization and expression analysis of three key starch synthesis-related genes from the bulb of a rare lily germplasm, Lilium brownii var. giganteum

Sun

et al. 2021

J. Zhejiang Univ. Sci. B

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Starch is the predominant compound in bulb scales, and previous studies have shown that bulblet development is closely associated with starch enrichment. However, how starch synthesis affects bulbification at the molecular level is unclear. In this study, we demonstrate that Lilium brownii var. giganteum, a wild lily with a giant bulb in nature, and L. brownii, the native species, have different starch levels and characteristics according to cytological and ultra-structural observations. We cloned the complete sequence of three key gene-encoding enzymes (LbgAGPS, LbgGBSS, and LbgSSIII) during starch synthesis by rapid amplification of 5' and 3' complementary DNA (cDNA) ends (RACE) technology. Bioinformatics analysis revealed that the proteins deduced by these genes contain the canonical conserved domains. Constructed phylogenetic trees confirmed the evolutionary relationships with proteins from other species, including monocotyledons and dicotyledons. The transcript levels of various tissues and time course samples obtained during bulblet development uncovered relatively high expression levels in bulblets and gradual increase expression accompanying bulblet growth. Moreover, a set of single nucleotide polymorphisms (SNPs) was discovered in the AGPS genes of four lily genotypes, and a purifying selection fashion was predicted according to the non-synonymous/synonymous (Ka/Ks) values. Taken together, our results suggested that key starch-synthesizing genes might play important roles in bulblet development and lead to distinctive phenotypes in bulblet size.

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Isolation and characterization of cDNAs and genomic DNAs encoding ADP-glucose pyrophosphorylase large and small subunits from sweet potato

Cited by 16 publications

References 40 publications

Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato

Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato

Identification of the conserved domains of ADP-Glucose Pyrophosphorylase (AGPase) protein in sweetpotato (Ipomoea batatas (L.) Lam.) and its two wild relatives

Molecular cloning, characterization and expression analysis of three key starch synthesis-related genes from the bulb of a rare lily germplasm, Lilium brownii var. giganteum

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