Isolation and characterization of EMS-induced Dy10 and Ax1 high molecular weight glutenin subunit deficient mutant lines of elite hexaploid wheat (Triticum aestivum L.) cv. Summit

Laudencia‐Chingcuanco, Debbie

doi:10.1016/j.jcs.2012.06.009

Cited by 8 publications

(7 citation statements)

References 18 publications

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“…As previously reported (Laudencia‐Chingcuanco, 2012) results indicate that dough derived from Dy10‐DLC achieved maximum resistance in a shorter time than the control. Mixing time for Dy10‐DLC decreased from 4.29 to 2.96 min in greenhouse‐grown plants and from 4.04 to 3.09 min in field‐grown plants (Table 1).…”

Section: Resultssupporting

confidence: 85%

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Registration of Dy10‐DLC Wheat

Laudencia‐Chingcuanco

2013

J of Plant Registrations

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Dy10‐DLC (Reg. No. GP‐964, PI 667096) wheat (Triticum aestivum L.) was developed by the USDA‐ARS at the Western Regional Research Center in Albany, CA. Dy10‐DLC was identified from an ethyl‐methanesulfonate‐mutagenized population of the elite hexaploid wheat cultivar Summit by screening M3 seeds for altered storage protein profiles using one‐dimensional SDS‐PAGE. Dy10‐DLC is distinct from the wild‐type progenitor line by the absence of the high‐molecular‐weight glutenin subunit (HMW‐GS) Dy10 protein. Examination by mixography of the effect of Dy10 protein deficiency on dough rheological properties showed shorter mixing time to reach peak resistance relative to the wild‐type control. The HMW‐GS proteins are major determinants of wheat dough processing qualities. The different alleles of the HMW‐GS genes in bread wheat vary in their effect on dough quality. Wheat lines deficient in the individual HMW‐GS will allow the determination of the contributions of each allele to dough quality.

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Section: Resultssupporting

confidence: 85%

“…Seeds at the M5 generation were used as the male parent for the initial backcross to the wild‐type Summit progenitor. The BC 1 F 2 seeds of Dy10‐DLC segregated in a 3:1 ratio indicating that the mutation is recessive and monogenic (Laudencia‐Chingcuanco, 2012).…”

Section: Resultsmentioning

confidence: 99%

Registration of Dy10‐DLC Wheat

Laudencia‐Chingcuanco

2013

J of Plant Registrations

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“…Seed at the M 5 generation was used as the male parent for the initial back cross to the wild‐type Summit progenitor. The BC 1 F 2 seeds of Ax1‐E28H segregated in a 3:1 ratio (21 normal:7 mutant), indicating that the mutation is recessive and monogenic (Laudencia‐Chingcuanco, 2012). The inheritance of the HMW‐GS Ax1 protein deficiency phenotype of Ax1‐E28H was stable, as evidenced by its consistent transmission to progenies of plants grown in the greenhouse and in two different field environments (Davis, CA, and Pullman, WA).…”

Section: Resultsmentioning

confidence: 99%

“…The same letter after values within each row designates nonsignificant difference based on Student's t test at P < 0.05. Data for greenhouse grown plants were as previously reported (Laudencia‐Chingcuanco, 2012).…”

Section: Resultsmentioning

confidence: 99%

“…To this end, a series of isogenic wheat lines with deficiencies in the HMW‐GS loci was isolated (unpublished data). A line deficient in the HMW‐GS Dy10 protein was previously reported and released (Laudencia‐Chingcuanco, 2012; Laudencia‐Chingcuanco, 2013). Here I register and report on several Ax1 protein deficient lines from mutagenized populations of the same elite bread wheat cultivar Summit: Ax1‐E28H (Reg.…”

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Registration of Isogenic HMW-GS Ax1 Protein Deficient Lines of an Elite Wheat Cultivar

Laudencia‐Chingcuanco

2015

Journal of Plant Registrations

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A series of isogenic wheat (Triticum aestivum L.) lines deficient in high‐molecular weight subunit (HMW‐GS) proteins encoded on the A‐genome of an elite cultivar of bread wheat was developed by USDA–ARS at the Western Regional Research Center in Albany, CA: Ax1‐E28H (Reg. No. GS‐179, PI 674178); Ax1‐F259C (Reg. No. GS‐180, PI 674179); Ax1‐F261B (Reg. No. GS‐181, PI 674180); Ax1‐F80B (Reg. No. GS‐182, PI 674181); Ax1‐F83B (Reg. No. GS‐183, PI 674182). This set of A‐genome encoded HMW‐GS deficient lines was identified from a chemical‐ and radiation‐mutagenized population of hexaploid wheat cultivar Summit by screening M3 seeds for altered storage protein profiles using one‐dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE). The mutant lines are distinct from the wild‐type progenitor by the absence of the HMW‐GS Ax1 protein. Dough derived from greenhouse‐ and field‐grown plants of one mutant exhibited weaker dough but achieved shorter development time compared with the control. Isogenic wheat lines deficient in the individual HMW‐GS will allow the determination of the contributions of the different alleles of each locus to dough quality.

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Post-translational cleavage of HMW-GS Dy10 allele improves the cookie-making quality in common wheat (Triticum aestivum)

Wang¹,

Chen

et al. 2021

Mol Breeding

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Wheat is a major staple food crop worldwide because of the unique properties of wheat our. High molecular weight glutenin subunits (HMW-GSs), which are among the most critical determinants of wheat our quality, are responsible for the formation of glutenin polymeric structures via interchain disul de bonds. We herein describe the identi cation of a new HMW-GS Dy10 allele (Dy10-m619SN). The amino acid substitution (serine-to-asparagine) encoded in this allele resulted in a partial posttranslational cleavage that produced two new peptides. These new peptides disrupted the interactions among gluten proteins because of the associated changes to the number of available cysteine residues for interchain disul de bonds. Consequently, Dy10-m619SN expression decreased the size of glutenin polymers and weakened glutens, which resulted in wheat dough with improved cookie-making quality, without changes to the glutenin-to-gliadin ratio. In this study, we clari ed the post-translational processing of HMW-GSs and revealed a new genetic resource useful for wheat breeding.

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Isolation and characterization of EMS-induced Dy10 and Ax1 high molecular weight glutenin subunit deficient mutant lines of elite hexaploid wheat (Triticum aestivum L.) cv. Summit

Cited by 8 publications

References 18 publications

Registration of Dy10‐DLC Wheat

Registration of Dy10‐DLC Wheat

Registration of Isogenic HMW-GS Ax1 Protein Deficient Lines of an Elite Wheat Cultivar

Post-translational cleavage of HMW-GS Dy10 allele improves the cookie-making quality in common wheat (Triticum aestivum)

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