Isolation and Characterization of Extremely Thermophilic Bacteria from Hot Springs

Abstract

Isolation and Characterization of Extremely Thermophilic Bacteria from Hot Springs

Isolation of Extrachromosomal Deoxyribonucleic Acids from Extremely Thermophilic Bacteria

“…This suggests that the plasmid DNAs have high deoxyguanosine and deoxycytosine contents like their chromosomal DNA (69 to 70%) (Oshima & Imahori, 1971;Saiki et al, 1972). The large plasmid (pTF2) was present as only a single copy, whereas there were many copies of the small plasmids (pTF1, pTT8, pTF61 and pTF62).…”

“…The establishment of in vitro recombinant DNA techniques in an extreme thermophile should reduce the risks since these bacteria are obligate aerobes, are not parasitic, not sporeforming, and grow only above 45 "C with an optimum growth temperature of 60 to 72 "C (Oshima & Imahori, 1971 ;Saiki, Kimura & Arima, 1972). Furthermore, Lindsay & Creaser (1975) reported that thermostability of an enzyme was a transformable property between Bacillus species.…”

Thermus oshimai sp. nov., Isolated from Hot Springs in Portugal, Iceland, and the Azores, and Comment on the Concept of a Limited Geographical Distribution of Thermus Species

“…The Gram reaction is negative, and the cell wall has an outer layer that balloons out from the inner peptidoglycan at regular intervals (3, 49). Thermus strains have been isolated from hot springs and other thermal environments in the United States (2, 4, 5, 27,35,44,53), Iceland (1, 7, 18, 21-23, 32, 42), Belgium (€9, Britain (31), Portugal (26,39,47,48), the Czech Republic (34), Germany (15), the former USSR (24,25), Japan (28,29,37,45,46), New Zealand (16, 17), Thailand (20), and Kenya (51). Thermus isolates are usually yellow pigmented because they contain carotenoids, but colorless strains have been isolated from sources sheltered from light (2, 22,31,35,39,44).…”

Fabrication of nanocomposites and hybrid materials using microbial biotemplates

“…Microbes are present in nature in all environments [8,9] including air, rivers, sea, soil, body of plants and animals, and even in extreme conditions such as craters [10] or hot springs [11]. These exist in nature in different shapes such as spheres, rods, spirals, and icosahedrons, etc.…”