Isolation and Characterization of Mannanase-producing Bacillus cereus Isolated from the Hindgut of Termites

Mairizal, Mairizal; Marlida, Yetti; Mirzah, Mirzah; Manin, Fahmida

doi:10.3923/pjn.2018.116.123

Cited by 5 publications

(4 citation statements)

References 22 publications

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“…Bakteri Lactobacillus plantarum adalah jenis BAL dari genus Lactobacillus yang sifatnya ramah lingkungan sebab bersifat non-patogen serta dapat mengganggu pertumbuhan bakteri patogen yang berperan sebagai probiotik. [6] telah berhasil mengisolasi Lactobacilus plantarum dari fermentasi durian (asam durian) yang dapat berfungsi sebagai probiotik.…”

Section: Pendahuluanunclassified

“…MOS dapat diproduksi dengan hidrolisis enzimatik BIS melalui penggunaan enzim mannanase yang diperoleh dari bakteri Bacillus cereus v9 [9]. Mikroba tersebut disolasikan melalui saluran pencernaan rayap mempunyai kemampuan memproduksi enzim mannanase melalui aktivitas enzimnya sejumlah 29,5 UI/mL [6]. Penggunaan MOS dapat meningkatkan efisiensi pakan karena MOS memberikan efek terhadap peningkatan tinggi vili dan jumlah sel goblet di jejunum ayam broiler [10].…”

Section: Pendahuluanunclassified

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Rasio Efisiensi Protein Ransum Ayam Broiler yang Diberi Lactobacillus plantarum dan Mannan Oligosakarida Hasil Hidrolisis Bungkil Inti Sawit

Fitrianingsih,

Mairizal,

Filawati

2023

J. Livest. Animal Health

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Tujuan dari penelitian ini ialah untuk mengetahui rasio efesiensi protein ransum pada ayam broiler yang diberi Lactobacillus plantarum dan Mannan oligosakarida (MOS) hasil hidrolisis bungkil inti sawit. Materi yang dipergunakan meliputi 270 ekor DOC strain MB 202 platinum. Ransum yang dipergunakan berupa ransum komersial Novo 511 non antibiotic growth promotor (non AGP). Rancangan yang digunakan ialah acak lengkap berpola faktorial 3 × 3 dengan 3 ulangan. Faktor A merupakan pemberian MOS hasil hidrolisis BIS dengan taraf yakni 0%, 0,50%, dan 1% dan faktor B merupakan pemberian Lactobacillus plantarum dengan taraf yakni 0%, 0,50%, dan 1%. Peubah yang diamati meliputi konsumsi ransum, konsumsi protein ransum, PBB serta rasio efisiensi protein. Data hasil penelitian dianalisis menggunakan ANOVA, nilai yang berbeda antar perlakuan diuji lanjut dengan DMRT 5%. Hasil penelitian menunjukan penambahan Lactobacillus plantarum, MOS, dan interaksi keduanya di dalam ransum memberikan pengaruh yang nyata (P<0,05) terhadap konsumsi ransum, konsumsi protein ransum, PBB serta rasio efisiensi protein ransum. Kesimpulan diperoleh adalah pemberian MOS hasil hidrolisis BIS sebanyak 1% melalui kombinasi Lactobacillus plantarum sebanyak 1% dalam ransum mampu meningkatkan rasio efisiensi protein ransum ayam broiler.

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Section: Pendahuluanunclassified

Rasio Efisiensi Protein Ransum Ayam Broiler yang Diberi Lactobacillus plantarum dan Mannan Oligosakarida Hasil Hidrolisis Bungkil Inti Sawit

Fitrianingsih,

Mairizal,

Filawati

2023

J. Livest. Animal Health

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“…The production of mannanase enzymes from microorganisms is superior because the production process is fast, the production conditions are more controlled, and the quality is uniform [8]. One of the microorganisms that can produce the mannanase enzyme is the bacterium Bacillus cereus V9 [9]. Bacillus cereus V9 is a mannanolytic bacteria isolated from the digestive tract of termites which is capable of producing mannanase enzymes on Locust bean gum (LBG) substrate with the enzyme activity of 29.5 U/mL 9 .…”

Section: Introductionmentioning

confidence: 99%

“…One of the microorganisms that can produce the mannanase enzyme is the bacterium Bacillus cereus V9 [9]. Bacillus cereus V9 is a mannanolytic bacteria isolated from the digestive tract of termites which is capable of producing mannanase enzymes on Locust bean gum (LBG) substrate with the enzyme activity of 29.5 U/mL 9 . According to Sumardi et al [10] the production of the enzyme mannanase usually uses a pure mannan substrate as a carbon source such as LBG.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Mannanase Enzymes Production from Bacillus cereus V9 Using Local Mannan Substrate

Mairizal¹,

Filawati²

2021

Proceedings of the 3rd Green Development International Conference (GDIC 2020)

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Bacillus cereus V9 is a mannanolytic bacteria capable of producing a mannanase enzyme of 29.5 IU / mL with a substrate of Locust bean gun. The use of Locust bean gum as a substrate in the production of the enzyme mannanase is not recommended because it is expensive so that the enzyme produced will be expensive. The use of local mannan substrates such as porang, coconut pulp, coconut cake, and palm kernel meal has the opportunity to replace Locust bean gum because the mannan content is almost equal to the content of the mannan Locust bean gum so the resulting enzyme is cheaper. This study aims to determine the best local mannan biomass substrate as a substitute for Locust bean gum for the production of the mannanase enzyme from Bacillus cereus V9 bacteria. This study consisted of two stages where the first stage was the optimization of the production of mannanase enzymes in various alternative substrates for Locust bean gum, then the second stage was the production of the enzyme mannanase from the selected substrate as a substitute for Locust bean gum. Simple data analysis is based on calculating the number and average of each observation from 4 replications and the data is displayed in the form of a descriptive narrative. The results showed that Bacillus cereus V9 could grow on all local mannan media and the highest was on palm kernel meal media. The growth of Bacillus cereus V9 at a substrate concentration of 1% palm kernel meal did not differ from a concentration of 1.5% and 2%. The activity of the mannanase enzyme Bacillus cereus V9 in palm kernel meal media was higher when compared to porang, coconut cake, and coconut pulp. This study concludes that palm kernel meal can be used as a 1% substrate as a substitute for Locust bean gum in the production of the enzyme mannanase from Bacillus cereus V9.

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Morphological and biochemical characterization of cellulase Bacterial from bearcat

Wibowo,

Fatimah

2024

International Conference on Organic and Applied Chemistry (Icoac) 2022

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Isolation and Characterization of Mannanase-producing Bacillus cereus Isolated from the Hindgut of Termites

Cited by 5 publications

References 22 publications

Rasio Efisiensi Protein Ransum Ayam Broiler yang Diberi Lactobacillus plantarum dan Mannan Oligosakarida Hasil Hidrolisis Bungkil Inti Sawit

Rasio Efisiensi Protein Ransum Ayam Broiler yang Diberi Lactobacillus plantarum dan Mannan Oligosakarida Hasil Hidrolisis Bungkil Inti Sawit

Optimization of Mannanase Enzymes Production from Bacillus cereus V9 Using Local Mannan Substrate

Morphological and biochemical characterization of cellulase Bacterial from bearcat

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