Isolation and characterization of mouse nasal-associated lymphoid tissue

“…Left lung tissue was excised and washed in HBSS. The head of the animal was processed to obtain nasal passages: the facial skin, cheek muscles, bones and palate were removed according to the method of Asanuma et al [3,4]. The remaining sections, consisting of nasal turbinates, septa, and lateral walls were obtained as nasal passage tissue.…”

Section: Experimental Protocolmentioning

confidence: 99%

Parenteral Nutrition Induces Organ Specific Alterations in Polymeric Immunoglobulin Receptor Levels

Sano

Gómez

Hermsen

et al. 2008

Journal of Surgical Research

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Background-Secretory IgA prevents pathogen adherence at mucosal surfaces to prevent infection. Polymeric immunoglobulin receptor (pIgR), located on the basolateral surface of mucosal cells, binds dimeric IgA produced by B cells with the cooperation of T cells in the lamina propria. This IgA-pIgR complex is transported apically, where it is exocytosed as secretory IgA to the mucosal surface. Our prior work shows that parenteral nutrition (PN) impairs both airway and small intestine mucosal immunity by reducing T & B cells and IgA levels. This work examines intestinal and respiratory tissue-specific pIgR responses to PN.

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“…Lymphoid cells from the O-NALT or D-NALT were obtained as described [39] and pooled from groups of six animals. The ELISPOT and ELISA assays were carried out as described using sucrose-purified RSV-A2 as the coating antigen [5].…”

Section: Rsv-specific Elispot and Elisamentioning

confidence: 99%

Intranasal immunisation with inactivated RSV and bacterial adjuvants induces mucosal protection and abrogates eosinophilia upon challenge

et al. 2006

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We have previously shown that following intranasal exposure to influenza virus, specific plasma cells are generated in the nasal-associated lymphoid tissue (NALT) and maintained for the life of the animal. However, we also showed that following infection with respiratory syncytial virus (RSV), specific plasma cells are generated in the NALT but wane quickly and are not maintained even after challenge, even though RSVspecific serum antibody responses remain robust. Only infection with influenza virus generated sterilising immunity, implying a role for these long-lived plasma cells in protection. We show here that the RSV-specific IgA NALT plasma cell population and lung antibody levels can be substantially boosted, both at acute and memory time points, by intranasal immunisation with inactivated RSV (iRSV) in combination with bacterial outer membrane vesicles (OMV) compared to live RSV alone. Finally, challenge with live RSV showed that immunisation with iRSV and OMV protect against both virus replication in the lung and the eosinophil infiltrate generated by either live RSV or iRSV alone. These data show that immunisation with iRSV and OMV maintains a NALT RSV-specific plasma cell population and generates an efficient protective immune response following RSV infection.See accompanying commentary: http://dx

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Isolation and characterization of mouse nasal-associated lymphoid tissue

Cited by 172 publications

References 19 publications

NK Cells Influence Both Innate and Adaptive Immune Responses after Mucosal Immunization with Antigen and Mucosal Adjuvant

NK Cells Influence Both Innate and Adaptive Immune Responses after Mucosal Immunization with Antigen and Mucosal Adjuvant

Parenteral Nutrition Induces Organ Specific Alterations in Polymeric Immunoglobulin Receptor Levels

Intranasal immunisation with inactivated RSV and bacterial adjuvants induces mucosal protection and abrogates eosinophilia upon challenge

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