Isolation and characterization of proteolytic microorganisms from fresh and fermented cabbage

Borla, Olinda Pérez; Davidovich, L. A.; Roura, Sara I.

doi:10.1016/j.lwt.2009.07.006

Cited by 19 publications

(8 citation statements)

References 28 publications

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“…Protease activity: Qualitative protease activity assay was evaluated on skim milk medium containing 5.6 g nutrient agar (Oxoid, UK) and 2.2 g sterile skim milk (Sigma Aldrich, USA) per 220 mL. The formation of clear zone (12 mm or more) in the medium surrounding the well indicated positive protease activity [14].…”

Section: Methodsmentioning

confidence: 99%

Protease Producers Predominate Cultivable Hydrolytic Bacteria Isolated from Liquid Biomedical Waste

Ethica¹,

Muchlissin²,

Saptaningtyas³

et al. 2018

Asian J. Chem.

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Hydrolytic bacteria are known for their crucial role in the bioconversion of organic matters mainly present in biomedical waste, making them advantageous in bioremediation. Analysis of abundance and colony diversity of culturable bacteria producing hydrolytic enzymes isolated from liquid biomedical waste of two different classes of hospitals in Semarang city, Central Java was carried out. Bacterial cultivation on nutrient agar slants resulted in total 26 viable colonies from inlet samples of Rumah Sakit Umum Roemani Muhammadiyah (R1) and Rumah Sakit Umum Daerah KRMT Wongsonegoro (R2) hospitals. Both hospitals represented two different classes of hospitals in Semarang (C and B, where B class is higher grade). Screening for production of four main hydrolytic enzymes (protease, lipase, amylase and cellulose) on all cultivable colonies obtained in this study. The results showed that all of them were capable of producing more than one type of hydrolytic enzymes. The composition was as follows: Protease producers (26 colonies), amylase producers (14 colonies), cellulose producers (13 colonies) and lipase producers (10 colonies).This study demonstrated that primary inlet of hospitals is a rich source of hydrolytic bacteria, where extracellular protease producers were found to be the main population of this group. In Semarang case, the inlet of lower class of hospital appeared to be more potential source of these hydrolytic bacteria than that of higher class one.

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Section: Methodsmentioning

confidence: 99%

Protease Producers Predominate Cultivable Hydrolytic Bacteria Isolated from Liquid Biomedical Waste

Ethica¹,

Muchlissin²,

Saptaningtyas³

et al. 2018

Asian J. Chem.

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“…The strain of B . cereus used in this study was isolated from fermented cabbage and has previously demonstrated high proteolytic activity . The culture was routinely maintained on soft Brain and Heart Infusion (BHI) agar (35 g/L of agar) at −18 °C.…”

Section: Methodsmentioning

confidence: 99%

“…095, Buenos Aires, Argentina) and 0.0025 yeast extract (Acumedia, lot no. 66‐22, Maryland, USA)] buffered at pH 8 with Tris–HCl 0.2 m . The inoculated flask was incubated at 37 °C for 24 h. The bacterial cells were harvested by centrifugation at 10,000 rpm for 10 min at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

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Characterization of a novel protease fromBacillus cereusand evaluation of an eco-friendly hydrolysis of a brewery byproduct

2015

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Proteases and proteolytic enzymes constitute one of the most important groups of enzymes and are attracting worldwide attention in attempts to exploit their physiological and biotechnological applications. In this study, partial purifications and biochemical and antimicrobial characterizations of a protease from Bacillus cereus spp., originally isolated from fermented cabbage, were carried out. The crude extract obtained after purification, involving ammonium sulphate precipitation and dialysis, was designated as a partially purified protease (PPP). The obtained PPP had a specific activity of 0.395-2.539 U/g at 32°C, with maximum activities for the fractions precipitated at 60 and 80% ammonium sulphate. The PPP activity ranged between 20 and 55°C, with an optimum temperature at 40°C. At 60°C, the PPP retained more than 30% of its activity. The optimum pH for the PPP was achieved at pH 9, indicating the alkaline source of the enzyme. Protease production was specifically dependent on the calcium concentration in the culture medium. Also the robustness of the protease on brewer's spent grain hydrolysis was demonstrated. This suggests a potential eco-friendly application of the enzyme. Finally, it was found that the PPP inhibited the growth of Escherichia coli O157:H7. This novel property of the PPP liberated by the B. cereus spp. could provide important future benefits to industry.

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“…Microbial protease are group of enzymes that can have apply in numerous industries, pharmaceutical process, food industry, oil extraction, etc. (Borla et al, 2010). Proteolytic bacteria play important role as culture starter for fermentation.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Lactic Acid Bacteria That Produce Protease and Bacteriocin-Like Substance From Mud Crab (Scylla sp.) Digestive Tract (Isolasi Bakteri Asam Laktat yang Menghasilkan Protease dan Senyawa Bacteriocin-Like dari Saluran Pencernaan Kepiting

2015

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Isolation and characterization of proteolytic microorganisms from fresh and fermented cabbage

Cited by 19 publications

References 28 publications

Protease Producers Predominate Cultivable Hydrolytic Bacteria Isolated from Liquid Biomedical Waste

Protease Producers Predominate Cultivable Hydrolytic Bacteria Isolated from Liquid Biomedical Waste

Characterization of a novel protease fromBacillus cereusand evaluation of an eco-friendly hydrolysis of a brewery byproduct

Isolation of Lactic Acid Bacteria That Produce Protease and Bacteriocin-Like Substance From Mud Crab (Scylla sp.) Digestive Tract (Isolasi Bakteri Asam Laktat yang Menghasilkan Protease dan Senyawa Bacteriocin-Like dari Saluran Pencernaan Kepiting

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