Isolation and characterization of Pseudomonas sp. strain capable of degrading diethylstilbestrol

Zhang, Weiwei; Niu, Zongliang; Liao, Chunyang; Chen, Lingxin

doi:10.1007/s00253-012-4648-1

Cited by 10 publications

(9 citation statements)

References 22 publications

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“…The first substance can be proposed to be DES-4-semiquinone, and the second substance was predicted to be [M+CH 3 COOH-H] – (M = DES-4-semiquinone). The results are in accordance with those reported by Zhang et al (2013). Acetic acid can be produced in the fermentation process but is easy to combine with DES-4-semiquinone due to its instability; thus, both DES-4-semiquinone and [DES-4-semiquinone+CH 3 COOH-H] – can be detected in the fermentation broth.…”

Section: Resultssupporting

confidence: 92%

“…Pseudomonas sp. J51 was isolated from seawater by Zhang et al (2013). The strain can degrade 80% of 10 mg/L DES in MSM medium in 1 day.…”

Section: Discussionmentioning

confidence: 99%

“…Presently, few studies are targeting biodegradation of DES, in particular, only two microorganisms have ever been reported to harbor the capability to degrade DES. Zhang et al (2013) screened a Pseudomonas sp. J51 strain with DES degradation ability.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of a Novel Diethylstilbestrol-Degrading Bacillus subtilis JF and Biochemical Degradation Metabolite Analysis

Deng

Yun

et al. 2019

Front. Microbiol.

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Diethylstilbestrol (DES) can adversely affect the immune system of developing fetuses or even elicit toxic responses such as nerve toxicity and genotoxicity in human beings, thereby warranting methods to remove DES from the environments. The present study characterized a novel DES-degrading Bacillus subtilis JF and analyzed the degradation metabolites. The strain was collected at the China General Microbiological Culture Collection Center (Collection number: CGMCC 7950). The environmental effects, such as DES concentrations, pH levels, and temperature, on the strain’s degradation ability were tested. Degradation metabolites of DES by strain JF were analyzed via high performance liquid chromatography (HPLC) and liquid-chromatography time of flight mass spectrometry (LC-TOF-MS). Results indicated that B. subtilis JF can effectively degrade DES within a concentration of 25–200 mg/L. Increasing pH levels (pH > 7) are reported to increase the degradation rate of DES by the strain. The optimal temperature for strain JF to degrade DES was identified as 45°C. In this study, 4, 4′-hexene estrogen quinones (DESQ) and DES-4-semiquinone were speculated as two degradation metabolites of DES, and both can be completely degraded by strain JF. A slight reduction of DES in the blank system [DES cultured in Luria-Bertani (LB) medium without strain JF] was observed in this study. The reduction trend in the blank system only occurred during the first few days (about 4 days) and was considerably lesser than the decomposition and transformation effect of DES via strain JF. Furthermore, the metabolite DESQ could not be further decomposed in blank LB medium without strain JF. All the results demonstrate that complete degradation of DES in the fermentation broth occurs due to the function of strain JF rather than organic decomposition. In conclusion, the high efficiency of degradation and the potential to degrade DES completely indicates that strain JF has potential for the bioremediation of DES-contaminated environments (soil, river, and so on) and fermented foods.

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Section: Resultssupporting

confidence: 92%

“…Pseudomonas sp. J51 was isolated from seawater by Zhang et al (2013). The strain can degrade 80% of 10 mg/L DES in MSM medium in 1 day.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and Characterization of a Novel Diethylstilbestrol-Degrading Bacillus subtilis JF and Biochemical Degradation Metabolite Analysis

Deng

Yun

et al. 2019

Front. Microbiol.

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“…Isolating functional strains that can mineralize estrogens directly may be useful in increasing the degradation efficiency of natural estrogens. Some estrogen-degrading strains have been reported in the last few decades [23,24,25,26,27]. However, only a few documented E2-degrading bacteria have been tested for their ability to remove E2 from cow manure, and isolation of more strains with high E2-degrading ability is still needed.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Characterization, and Degradation Performance of the 17β-Estradiol-Degrading Bacterium Novosphingobium sp. E2S

Liu

Sun

et al. 2017

IJERPH

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A 17β-estradiol (E2)-degrading bacterium E2S was isolated from the activated sludge in a sewage treatment plant (STP). The morphology, biological characteristics, and 16S ribosomal RNA (rRNA) gene sequence of strain E2S indicated that it belonged to the genus Novosphingobium. The optimal degrading conditions were 30 °C and pH 7.0. The ideal inoculum volume was 5% (v/v), and a 20-mL degradation system was sufficient to support the removal ability of strain E2S. The addition of extra NaCl to the system did not benefit the E2 degradation in batch culture by this strain. Strain E2S exhibited high degradation efficiency with initial substrate concentrations of 10–50 mg·L−1. For example, in mineral salt medium containing 50 mg·L−1 of E2, the degradation efficiency was 63.29% after seven days. In cow manure samples supplemented with 50 mg·L−1 of E2, strain E2S exhibited 66.40% degradation efficiency after seven days. The finding of the E2-degrading strain E2S provided a promising method for removing E2 from livestock manure in order to reduce the potential environmental risks of E2.

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“…P. putida KA4 and KA5 were confirmed with high degradation abilities to another EEs member, bisphenol A (BPA) 22 . And some Pseudomonas strains can biotransform diethylstilbestrol and 4-nonylphenol 23 24 .…”

mentioning

confidence: 99%

iTRAQ-based quantitative proteomic analysis of the global response to 17β-estradiol in estrogen-degradation strain Pseudomonas putida SJTE-1

Zhang

Hou

et al. 2017

Sci Rep

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Microorganism degradation is efficient to remove the steroid hormones like 17β-estradiol (E2); but their degradation mechanism and metabolic network to these chemicals are still not very clear. Here the global responses of the estrogen-degradation strain Pseudomonas putida SJTE-1 to 17β-estradiol and glucose were analyzed and compared using the iTRAQ (isobaric tags for relative and absolute quantization) strategy combined with LC-MS/MS (liquid chromatography-tandem mass spectrometry). 78 proteins were identified with significant changes in expression; 45 proteins and 33 proteins were up-regulated and down-regulated, respectively. These proteins were mainly involved in the processes of stress response, energy metabolism, transportation, chemotaxis and cell motility, and carbon metabolism, considered probably responding to 17β-estradiol and playing a role in its metabolism. The up-regulated proteins in electron transfer, energy generation and transport systems were thought crucial for efficient uptake, translocation and transformation of 17β-estradiol. The over-expression of carbon metabolism proteins indicated cells may activate related pathway members to utilize 17β-estradiol. Meanwhile, proteins functioning in glucose capture and metabolism were mostly down-regulated. These findings provide important clues to reveal the 17β-estradiol degradation mechanism in P. putida and promote its bioremediation applications.

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Isolation and characterization of Pseudomonas sp. strain capable of degrading diethylstilbestrol

Cited by 10 publications

References 22 publications

Isolation and Characterization of a Novel Diethylstilbestrol-Degrading Bacillus subtilis JF and Biochemical Degradation Metabolite Analysis

Isolation and Characterization of a Novel Diethylstilbestrol-Degrading Bacillus subtilis JF and Biochemical Degradation Metabolite Analysis

Isolation, Characterization, and Degradation Performance of the 17β-Estradiol-Degrading Bacterium Novosphingobium sp. E2S

iTRAQ-based quantitative proteomic analysis of the global response to 17β-estradiol in estrogen-degradation strain Pseudomonas putida SJTE-1

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