Isolation and characterization of salt-tolerant rhizobia native to the desert&lt;br&gt;soils of United Arab Emirates

Sharma, Samrudhi R.; Rao, N. Kameswara; Gokhale, Trupti; Ismail, Shoaib

doi:10.9755/ejfa.v25i2.7590

Cited by 33 publications

(25 citation statements)

References 12 publications

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“…According to this particular research there was positive correlation between in vitro stress tolerance and %SE of TCP solubilizer rhizobia isolate at P<0.5. This result was similar to Sharma et al (2013) who concluded that invitro stress tolerance is an indication for the survival and persistence of rhizobial isolates under severe and harsh desert conditions of real in vivo condition. Keyser et al (1979) also reported the positive correlation between Al toxicity tolerances and symbiotic effectiveness of some strains.…”

Section: Resultssupporting

confidence: 90%

Characterization of Phosphate Solubilizing Faba Bean (<i>Vicia faba</i> L.) Nodulating Rhizobia Isolated from Acidic Soils of Wollega, Ethiopia

Kenasa¹,

Jida²,

Assefa³

2014

Sci. Technol. Arts Res. J.

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Article InformationSome species of rhizobial bacteria nodulating faba bean are characterized by phosphate solubilization. In order to study their in vitro and symbiotic characteristics, twelve rhizobial isolates nodulating faba bean were collected from acidic soil of Wollega, Ethiopia. Solubilization index of the isolates ranges from 1.25 to 2.10. Mean Generation Time of the isolates were less than 2.34; and growth of isolates on Yeast Extract Mannitol agar with bromothymoleblue media were accompanied by change of color from blue to yellow. AUAVR-51 and AUAVR-52 were the highest in TCP-Solubilizion Index. The two isolates were also recognized as stress tolerant when tested in vitro for extreme temperature, osmotic, acidic pH, intrinsic antibiotics, and acidic-aluminum as compared to the other isolates. However, there was no unique metabolic diversity and specialization of AUAVR-51 and AUAVR-52 isolates with respect to carbon and nitrogen source utilization. On the other hand, isolates AUAVR-51 and AUAVR-52 were characterized by effective and highly effective symbiosis on sterile potted sand growth, respectively. In general, phosphate solubilizing rhizobia nodulating faba bean from acidic soil are fast grower; and their solubilization potential varies. However, the contribution of these isolates as double fertilizer should be tested in the real acidic soil characterized by immobilized phosphorous.

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Section: Resultssupporting

confidence: 90%

Characterization of Phosphate Solubilizing Faba Bean (<i>Vicia faba</i> L.) Nodulating Rhizobia Isolated from Acidic Soils of Wollega, Ethiopia

Kenasa¹,

Jida²,

Assefa³

2014

Sci. Technol. Arts Res. J.

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“…S elec tion of the highes t s alt tol erat e is olate and inoc ulated with specific l egumes enhance t he nodulation and nitrogen fi xation effici enc y of l eguminous plants c ultiv at ed under extrem e envir onm ents (Sharma et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Efficiency of nitrogen fixation by lysogenic and non-lysogenic isolates of Rhizobium nodulated Sesbania aegyptica.

Hashem¹

2017

Egypt. J. Exp. Biol. (Bot.)

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Efficiency of nitrog en fi xation by ly sogenic and non -ly sogeni c isolates of Rhiz obium nodul ated Se sbani a aeg yptica ABSTRACT:Six Sesbania nodulating rhizobia designated as (RS1, RS2, RS3, RS4, RS5, and RS6) isolated from El-Garbia and El-Menoufia governorates were characterized. These isolates were fast growing Rhizobium and produced Indole acetic acid with different concentrations. Furthermore, all these isolates were varied in their response to different concentrations of NaCl and Intrinsic antibiotic resistance pattern. Lysogenicity in the isolated Sesbania nodulating rhizobia was tested. One lysogenic isolate RS2 (RSL) was identified among the tested six Sesbania Rhizobium, this isolate produced turbid plaques. Single plaque was picked and propagated and designated as (ϕB5). Electron microscopy results suggested that ϕB5 belong to family Myoviridae having icosahedral head (65.27 ± 5.09 nm), long contractile tail (91.4 ± 1.3, 17.93 ± 1.25 nm) and neck (21.0 ± 1.3, 4.2 ± 0.0 nm) and has approximately 23.5 Kbp of dsDNA. Further incubation time of lytic area performed by plating of RSL with ϕB5 lead to appearance of resistant Rhizobium colony (RP5). This purified colony was non-lysogenic Rhizobium. The symbiotic interaction of RSL (phage sensitive) and RP5 (phage resistance) was studied using Sesbania aegyptica as a host in presence and absence of phages. Assay of symbiotic efficiency based on nodule number, nitrogenase activity, dry weight and total nitrogen of shoot system was detected. Symbiotic effectiveness in phage resistant nonlysogenic isolate was higher than in lysogenic phage sensitive isolate in presence of phages in soil. The present study showed a significant relationship between the phage sensitivity and symbiotic efficiency of the isolated Rhizobium. KEY WORDS:Nitrogen fixation, lysogenic and non-lysogenic isolates, Rhizobium nodulated Sesbania aegyptica CORRESP ONDENCE: E m an A . M. H at em Botany department,

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“…No entanto, uma série de fatores afetam a relação simbiótica rizóbio-leguminosa. Estes incluem a compatibilidade com a planta hospedeira e as condições físico-químicas do solo, especialmente salinidade e pH do solo, deficiência de nutrientes, toxicidade de minerais e metais pesados, temperaturas extremas, umidade do solo insuficiente ou excessiva e fatores bióticos tais como a as interações com outros microrganismos do solo (PEREIRA et al, 1999;SHARMA et al, 2013;IYER ;RAJKUMAR, 2017).…”

Section: Rizóbiosunclassified

Interações Bióticas Entre Actinobactérias E Rizóbios Em Solos Da Região Semiárida Brasileira

Cavalcante¹,

Martins²,

Martins³

2017

Enci Bio

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RESUMOExistem várias interações ocorrendo no solo que determinam as propriedades do mesmo como um meio para atividade e crescimento de plantas e microrganismos. Há diversos tipos diferentes de microrganismos na rizosfera interagindo com os outros organismos do solo e com as raízes das plantas. As actinobactérias são de ocorrência universal na natureza e são amplamente distribuídas em ambientes naturais. As bactérias diazotróficas, também chamadas de rizóbios, realizam o processo de fixação biológica do nitrogênio (FBN) sendo uma importante fonte de nitrogênio para o solo. Dados da literatura relatam antagonismo das actinobactérias sobre vários microrganismos do solo incluindo os rizóbios. As interações bióticas conduzem a processos ecológicos e evolutivos fundamentais, porém a sua intensidade, direção e frequência são sensíveis às mudanças climáticas. O semiárido brasileiro é uma região caracterizada por baixa precipitação anual, temperaturas elevadas e vegetação caducifólia. Os recursos nos solos dessa região são geralmente limitantes para os microrganismos, o que favorece a competição. PALAVRAS-CHAVE: Antagonismo, Coexistência, Microrganismos do solo BIOTIC INTERACTIONS BETWEEN ACTINOBACTERIAS AND RHIZOBIA IN SOILS OF THE BRAZILIAN SEMI-ARID REGIONABSTRACT There are several interactions occurring in the soil that determine its properties as a medium for the activity and growth of plants and microorganisms. There are different types of microorganisms in the rhizosphere interacting with other soil organisms and with plant roots. Actinobacteria universally occur in nature and are widely distributed in natural environments. Diazotrophic bacteria, also known as rhizobia, perform the biological nitrogen fixation process (BNF) being an important source of nitrogen for the soil. The scientific literature reports antagonism of actinobacteria on various soil

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Isolation and characterization of salt-tolerant rhizobia native to the desert<br>soils of United Arab Emirates

Cited by 33 publications

References 12 publications

Characterization of Phosphate Solubilizing Faba Bean (<i>Vicia faba</i> L.) Nodulating Rhizobia Isolated from Acidic Soils of Wollega, Ethiopia

Characterization of Phosphate Solubilizing Faba Bean (<i>Vicia faba</i> L.) Nodulating Rhizobia Isolated from Acidic Soils of Wollega, Ethiopia

Efficiency of nitrogen fixation by lysogenic and non-lysogenic isolates of Rhizobium nodulated Sesbania aegyptica.

Interações Bióticas Entre Actinobactérias E Rizóbios Em Solos Da Região Semiárida Brasileira

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