Isolation and characterization of spermatogenic cells from cattle, yak and cattleyak

Shah, Mujahid Ali; Xu, Cheng; Wu, Shan; Zhao, Wangsheng; Luo, Haiwei; Yi, Chuanping; Liu, Wenjing; Cai, Xin

doi:10.1016/j.anireprosci.2018.04.067

Cited by 35 publications

(39 citation statements)

References 33 publications

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“…The process of testicular tissue collecting from yaks (n = 3) and cattleyaks (n = 3) for cell isolation is the same as that described above. We isolated the spermatogenic cells (including spermatogonia and spermatocytes) from these two bovid species using STA-PUT velocity sedimentation, and the protocol was performed by referring to our previous study [20]. Firstly, the spermatogenic cells were preliminarily identified based on the morphological characteristics and size differences at different spermatogenic stages.…”

Section: Isolation and Identification Of Spermatogenic Cells From Tesmentioning

confidence: 99%

“…Spermatogenic cells comprised spermatogonia and spermatocytes were isolated from yak and cattleyak using STA-PUT velocity sedimentation [20]. For the yak, the percentage of spermatogonia ( Fig 5A) and spermatocytes ( Fig 5B) was 75.36% and 24.64%, respectively; while the percentage of spermatogonia ( Fig 5C) and spermatocytes ( Fig 5D) for the cattleyak was 79.71% and 20.29%, respectively (S4 Fig, S8 Table).…”

Section: Identification Of Spermatogenic Cells From Yak and Cattleyakmentioning

confidence: 99%

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Testis transcriptome profiling identified genes involved in spermatogenic arrest of cattleyak

Mipam

et al. 2020

PLoS ONE

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Background Cattleyak are the hybrid offspring between cattle and yak and combine yak hardiness with cattle productivity. Much attempt has been made to examine the mechanisms of male sterility caused by spermatogenic arrest, but yet there is no research systematically and precisely elucidated testis gene expression profiling between cattleyak and yak. Methods To explore the higher resolution comparative transcriptome map between the testes of yak and cattleyak, and further analyze the mRNA expression dynamics of spermatogenic arrest in cattleyak. We characterized the comparative transcriptome profile from the testes of yak and cattleyak using high-throughput sequencing. Then we used quantitative analysis to validate several differentially expressed genes (DEGs) in testicular tissue and spermatogenic cells. Results Testis transcriptome profiling identified 6477 DEGs (2919 upregulated and 3558 downregulated) between cattleyak and yak. Further analysis revealed that the marker genes and apoptosis regulatory genes for undifferentiated spermatogonia were upregulated, while the genes for differentiation maintenance were downregulated in cattleyak. A majority of DEGs associated with mitotic checkpoint, and cell cycle progression were downregulated in cattleyak during spermatogonial mitosis. Furthermore, almost all DEGs related to synaptonemal complex assembly, and meiotic progression presented no sign of expression in cattleyak. Even worse, dozens of genes involved in acrosome formation, and flagellar development were dominantly downregulated in cattleyak. Conclusion DEGs indicated that spermatogenic arrest of cattleyak may originate from the differentiation stage of spermatogonial stem cells and be aggravated during spermatogonial mitosis

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Section: Isolation and Identification Of Spermatogenic Cells From Tesmentioning

confidence: 99%

Section: Identification Of Spermatogenic Cells From Yak and Cattleyakmentioning

confidence: 99%

Testis transcriptome profiling identified genes involved in spermatogenic arrest of cattleyak

Mipam

et al. 2020

PLoS ONE

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“…Additionally, the STA-PUT velocity sedimentation allowed for the separation of spermatogonia, pachytene spermatocytes and round spermatids in mice [6,7] and human [8]. Recently, we have also conducted the analogous research to obtain spermatogonia and spermatocytes from CL, YK and CY by STA-PUT velocity sedimentation with over 85% purity [9].…”

Section: Introductionmentioning

confidence: 99%

Bovid microRNAs involved in the process of spermatogonia differentiation into spermatocytes

Xu¹,

Shah²,

Mipam³

et al. 2020

Int. J. Biol. Sci.

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The male infertility of cattleyak resulted from spermatogenic arrest has greatly restricted the effective utilization of the heterosis from crossbreeding of cattle and yak. Based on our previous studies, the significant divergences of the transcriptomic and proteomic sequencing between yak and cattleyak prompt us to investigate the critical roles of microRNAs in post-transcriptional regulation of gene expression during spermatogenesis. TUNEL-POD analysis presented sharply decreased spermatogenic cell types and the increased apoptotic spermatogonia in cattleyak. The STA-PUT velocity sedimentation was employed to obtain spermatogonia and spermatocytes from cattle, yak and cattleyak and these spermatogenic cells were verified by the morphological and phenotypic identification. MicroRNA microarray showed that 27 differentially expressed miRNAs were simultaneously identified both in cattleyak vs cattle and in cattleyak vs yak comparisons. Further analysis revealed that the down-regulation of bta-let-7 families, bta-miR-125 and bta-miR-23a might impair the RA-induced differentiation of spermatogonia. Target gene analysis for differentially expressed miRNAs revealed that miRNAs targeted major players involved in vesicle-mediated transport, regulation of protein kinase activity and Pathways in cancer. In addition, spermatogonia transfection analysis revealed that the down-regulation of bta-miR-449a in the cattleyak might block the transition of male germ cells from the mitotic cycle to the meiotic program. The present study provided valuable information for future elucidating the regulatory roles of miRNAs involved in spermatogenic arrest of cattleyak.

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“…In addition, yaks provide locals with a means of transportation [7]. Yak thus play an important socioeconomic role in regions where they are endemic, being vital in maintaining pasture ecosystems and agricultural biodiversity in plateau areas [8]; hence, yaks are referred to as "all-around animals" [2]. However, yak populations are currently undergoing degradation, leading to an urgent need to cultivate new breeds.…”

Section: Introductionmentioning

confidence: 99%

Mitogenomic Diversity and Maternal Origins of Yak (Bos Grunniens)

Wang¹,

Pei²,

Bao³

et al. 2021

Preprint

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Background and aim: Mitochondrial genome has the characteristics of simple structure, no recombination, maternal inheritance, high conservation, fast evolution, and high copy number. It is easy to sequence, contains high resolution phylogenetic information, and exists in a wide range of taxa. Theref ore, it is widely used in the study of biological phylogeny. At present, phylogenetic studies focus mainly on D loop region, cytochrome b gene, and protein coding sequence; phylogenetic studies using the mitochondrial complete sequence are rare. Therefore, this study aimed to conduct phylogenetic experiments using the mitochondrial complete sequence and compare the results with previous findings obtained using partial sequences. Results: Complete mitochondrial sequences of five yak populations from Qinghai and Xinjiang were obtained. The haplotype diversity of the five populations were Xueduo yak (0.992 ± 0.015), Pamir yak (0.990 ± 0.014), Yushu yak (0.963 ± 0.033), Qilian yak (0.948 ± 0.036), and Huanhu yak (0.905 ± 0.048), which showed a higher haplotype diversity compared with other breeds. A total of 78 haplotypes were obtained from 111 individuals. Among these, Yushu yak, Huanhu yak, Xueduo yak, and Qilian yak all shared haplotypes, but the Pamir yak did not share haplotypes with these four populations. Phylogenetic analysis showed that yak populations were separable into three distinct branches. The analysis identified a new phylogenetic branch with both wild and domestic yaks being represented in the new branch. The 155 haplotypes were divided into 6 haplotype groups by haplotype clustering. The haplotype group had nothing to do with the morphological group of yaks, and yaks from the same population or the same ecological group were distributed in different haplotype groups. The four haplotype groups A, B, C, and D, showed a star-shaped distribution of haplotypes. The central haplotypes were widely distributed and had a high frequency Conclusions: T he genetic diversity of yaks in Qinghai was high. Both domestic and wild yaks exhibited three different mat ernal origins. Three different maternal origins existed in ancient wild yaks before domestication, with haplogroup A being the primary haplogroup associated with yak domestication.

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Isolation and characterization of spermatogenic cells from cattle, yak and cattleyak

Cited by 35 publications

References 33 publications

Testis transcriptome profiling identified genes involved in spermatogenic arrest of cattleyak

Testis transcriptome profiling identified genes involved in spermatogenic arrest of cattleyak

Bovid microRNAs involved in the process of spermatogonia differentiation into spermatocytes

Mitogenomic Diversity and Maternal Origins of Yak (Bos Grunniens)

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