Isolation and characterization of the folate-binding protein from cow's milk

Svendsen, Ib; Martin, Brian M.; Pedersen, Torben Graves; Hansen, Steen Ingemann; Holm, Jan; Lyngbye, Jörgen

doi:10.1007/bf02906524

Cited by 62 publications

(44 citation statements)

References 27 publications

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“…It was sequenced 12 steps starting at Phe64 together with an already known sequence starting at Lys4_,. CNBr-III starting at Glu73 was previously sequenced 30 steps through Gln~02 (16). This sequence was repeated and extended to include Cys,~9.…”

Section: Resultsmentioning

confidence: 99%

“…The C-terminal CNBr-fragment has already been published (16) except for the final positions of the Pro and Gln in positions 219 and 220. A peptide purified from the digest of FBP by S. aureus V8 protease was sequenced nine steps and included Pro2,9.…”

Section: Resultsmentioning

confidence: 99%

“…The preparation and purification of cyanogen bromide fragments from reduced and vinylethylated FBP have already been described (16). The four resulting fragments are numbered in the order in which they appear in the sequence (CNBr-I to CNBr-IV).…”

Section: Cyanogenbromide Fragmentsmentioning

confidence: 99%

“…Proteins which specifically bind folate have been isolated from various body fluids and tissues of mammals (1,7,12,14,15,16,18,19,20) and also from bacteria (9), In order to understand the function of these proteins it is important to know their structures, In a previous communication we have shown the partial amino acid sequence of the folate binding protein from cow's milk based on the cyanogen bromide fragments (16). Homology between this protein and the high and low molecular weight folate binding protein from human milk has been demonstrated (17).…”

Section: Introductionmentioning

confidence: 99%

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The complete amino acid sequence of the folate-binding protein from cow's milk

Svendsen

Hansen

Holm

et al. 1984

Carlsberg Res. Commun.

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The complete amino acid sequence of the folate binding protein from cow's milk has been determined by automated sequencing of fragments obtained by cleavage with cyanogen bromide, trypsin, S. aureus V8 protease and clostripain. The molecule consists of a single polypeptide chain of 222 amino acid residues and eight disulphide bridges. The molecular weight of the protein part is 25,700. The folate binder contains carbohydrate which gives it a final molecular weight of about 30,000. No sequence homology with other proteins has been observed.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Cyanogenbromide Fragmentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The complete amino acid sequence of the folate-binding protein from cow's milk

Svendsen

Hansen

Holm

et al. 1984

Carlsberg Res. Commun.

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“…In a previous report (12) the isolation of a folate-binding protein (FBP) from cow's milk and some of its properties were described. The molecular weight was found to be 30,000 at pH 5.0.…”

Section: Introductionmentioning

confidence: 99%

Aggregation of a folate-binding protein from cow's milk

Pedersen

Svendsen

Hansen

et al. 1980

Carlsberg Res. Commun.

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Sedimentation equilibrium experiments of folate-binding protein from cow's milk performed in the analytical ultracentrifuge at pH 5 9 showed a single molecular species with a molecular weight of 30,000 9 In contrast, at pH 7.4 sedimentation equilibrium indicated molecular weights ranging from about 30,000 to at least 300,000. OSedimentation velocity experiments at pH 5.0 showed a single symmetrical peak with s20,w = 2.8 S in agreement with a molecular weight of about 30,000. At pH 6.0 was seen a faster moving boundary (s20,w~ 6S) in addition to the slow one. At higher pH two peak maxima were seen: a slow one with sedimentation coefficients of about 5S and a fast one increasing from 9S to 1 IS with increasing pH. Addition of folate further increased the sedimentation rates. By assuming that G.LBERT'S rapid monomer-n-mer equilibrium theory was valid also for a lower polymer in equilibrium with a higher polymer our data can be interpreted as an equilibrium between a tetramer and a polymer consisting of more than 16 monomers. In the case of folate addition the results may similarly be interpreted as an equilibrium between an octamer and a polymer composed of more than 32 monomers.

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Chemistry of Whey Proteins

Guo

Wang

2019

Whey Protein Production, Chemistry, Functionality, and Applications

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Isolation and characterization of the folate-binding protein from cow's milk

Cited by 62 publications

References 27 publications

The complete amino acid sequence of the folate-binding protein from cow's milk

The complete amino acid sequence of the folate-binding protein from cow's milk

Aggregation of a folate-binding protein from cow's milk

Chemistry of Whey Proteins

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