Isolation and enumeration of bacteria responsible for nosocomial infections from houseflies and determining their susceptibility to poison bait

Abstract

Abstract. Haeidari A, Keshavarzi D, Owlia P, Vatandoost H, Rafinejad A, Rafinejad J. 2020. Isolation and enumeration of bacteria responsible for nosocomial infections from houseflies and determining their susceptibility to poison bait. Nusantara Bioscience 13: 24-28. Nosocomial infections represent a serious public health concern in developing countries. Houseflies are one of the most common household pests carrying different pathogenic organisms. The purpose of this study was to isolate and enumerate bacteria species from house flies and to determine their susceptibility to Agita® fly bait. Flies were collected from two hospital environments between July to December 2014, in Yazd Province of Iran. Bacterial species were isolated from the outer surfaces of flies, and Agita® efficacy was evaluated based on lethal time (LT50) after 1, 2, 4, 8, 16, and 32 minutes. Three species of bacteria (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus) responsible for nosocomial infections have been isolated and enumerated from flies. Among the 30 flies collected, 17, 24, and 3 flies were contaminated with E. coli, S. aureus, and Ps. aeruginosa, respectively. The minimum number of isolated bacteria was 3 × 102 CFU/mL, whereas the highest number was 2.4 × 105 CFU/mL. The susceptibility results showed that, despite the existence of a significant effect for heterogeneity in both field and laboratory strains (p-value ˂ 0/05) and an increase in the mortality of houseflies during the time, there was no significant difference between two strains regarding the efficacy of Agita® against houseflies. The findings of the present study revealed and confirmed that houseflies have an important role in the spread of nosocomial infections in hospital environments, and they are susceptible to Agita® fly bait.

Monitoring the effectiveness of surgical handwashing in healthcare workers of a fourth level operating room using bioluminescence assays

“…On the other hand, E. coli has been isolated in a lower proportion on HCW hands [35], [45], [46]; however, it is important to highlight that E. coli is listed as one of the most important pathogenic bacteria causing nosocomial infections. In fact, the increasing antibiotic multidrug resistance of this bacteria has created many problems [47]. It has been described that coliforms are fecal bacteria and their presence suggests that food and water supplies could be the source of contamination [48].…”

Short Communication: Bacillus endolithicus and Bacillus paramycoides: New isolates from housefly Musca domestica in Saudi Arabia

“…Moreover, Khamesipour et al (2018) isolated 130 pathogenic organisms from the houseflies in which bacteria were the most frequent. Also, Haeidari et al (2021) studied the bacterial diversity isolated from the external surface of house flies collected from the hospitals in Yazd Province of Iran. They declared the presence of three species responsible for nosocomial infections such as Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus.…”