Isolation and evaluation of immunological adjuvant activities of saponins from the roots of Pulsatilla chinensis with less adverse reactions

Sun, Yeqing; Liu, Jicheng; Yu, Haitao; Gong, Chunjie

doi:10.1016/j.intimp.2010.02.006

Cited by 55 publications

(29 citation statements)

References 51 publications

(61 reference statements)

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“…Meyer [3]. Their pharmacological activities, including anti-inflammatory [4], hypoglycemic [5], immunological adjuvant [6], and anti-endotoxin [7] properties, have been well studied. Various methods including UV spectrophotometry [8] and chromatography [9] coupled with different kinds of detectors have been reported for the determination of TSs in traditional Chinese medicines (TCMs).…”

Section: Introductionmentioning

confidence: 99%

Fast separation of triterpenoid saponins using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

Huang

Zhang

Zhou

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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a b s t r a c tTriterpenoid saponins (TSs) are the most important components of some traditional Chinese medicines (TCMs) and have exhibited valuable pharmacological properties. In this study, a rapid and efficient method was developed for the separation of kudinosides, stauntosides and ginsenosides using supercritical fluid chromatography coupled with single quadrupole mass spectrometry (SFC-MS). The separation conditions for the selected TSs were carefully optimized after the initial screening of eight stationary phases. The best compromise for all compounds in terms of chromatographic performance and MS sensitivity was obtained when water (5-10%) and formic acid (0.05%) were added to the supercritical carbon dioxide/MeOH mobile phase. Beside the composition of the mobile phase, the nature of the make-up solvent for interfacing SFC with MS was also evaluated. Compared to reversed phase liquid chromatography, the SFC approach showed higher resolution and shorter running time. The developed SFC-MS methods were successfully applied to the separation and identification of TSs present in Ilex latifolia Thunb., Panax quinquefolius L. and Panax ginseng C.A. Meyer. These results suggest that this SFC-MS approach could be employed as a useful tool for the quality assessment of natural products containing TSs as active components.

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Section: Introductionmentioning

confidence: 99%

Fast separation of triterpenoid saponins using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

Huang

Zhang

Zhou

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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show abstract

“…Adjuvants should also offer excellent safety, tolerability, ease of manufacture and formulation. Therefore, modern adjuvants should have a profound effect on the nature of the immune response and it is often desirable to induce specific types of immunity (34), which may bias the immune system toward either a Th1 or a Th2 type response (35). Among the T lymphocytes, T cell responses have been divided in two subclasses, Th1 and Th2.…”

Section: Discussionmentioning

confidence: 99%

“…IL-2, TNF-β and IFN-γ vs. IL-4, IL-5 and IL-10, respectively. Th1 cells protect against intracellular pathogens, activate phagocytosis, induce IgG2a, IgG2b and IgG3 antibodies and promote delayed-type hypersensitivity responses, whereas Th2 cells protect against extracellular pathogens, activate eosinophils, induce IgE-mediated allergic responses and promote other humoral responses in which IgG1, IgGE and IgA predominate (35). Polarized Th1 and Th2 phenotypes are critical in the course of immune responses, and efficient Th1 and Th2 immunity-inducing adjuvants, particularly those for Th1, are highly in demand (2).…”

Section: Discussionmentioning

confidence: 99%

A polysaccharide isolated from Agaricus blazei Murill (ABP-AW1) as a potential Th1 immunity-stimulating adjuvant

Cui

Sun

et al. 2013

Oncology Letters

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In the present study, a low molecular weight polysaccharide, ABP-AW1, isolated from Agaricus blazei Murill was assessed for its potential adjuvant activity. ABP-AW1 is considered to create a ‘depot’ of antigen at a subcutaneous injection site. ICR mice were immunized with 100 μg ovalbumin (OVA) alone or with 100 μg OVA formulated in 0.9% saline containing 200 μg aluminum (alum) or ABP-AW1 (50, 100 and 200 μg) on days 1 and 15. Two weeks after the secondary immunization, splenocyte proliferation, the expression of surface markers, cytokine production and the OVA-specific antibody levels in the serum were determined. The OVA/ABP-AW1 vaccine, in comparison with OVA alone, markedly increased the proliferation of splenic lymphocytes and elicited greater antigen-specific CD4+ T cell activation, as determined by splenic CD4+CD69+ T cells and Th1 cytokine interferon (IFN)-γ release. The combination of ABP-AW1 and OVA also enhanced IgG2b antibody responses to OVA. In conclusion, these data indicated that ABP-AW1 significantly enhanced the humoral and cellular immune responses against OVA in the mice, suggesting that ABP-AW1 stimulated Th1-type immunity. We suggest that ABP-AW1 may serve as a new adjuvant.

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“…The triterpenoid saponins in P. chinensis have demonstrated multiple pharmacological activities including anti-tumor [1-7], cognition-enhancing [8,9], immuno-modification [10], immunological adjuvant [11], anti-oxidant [12], breech presentation [13], anti-inflammatory [14], and antiendotoxin [15] activities. Pentacyclic triterpenes are the main compounds found in P. chinensis extracts, such as 23-hydroxybetulinic acid, which is the major active constituent of P. chinensis [16,17].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Triterpenoidal Saponins in Rat Urine After Oral Administration of Pulsatilla chinensis Extract Using HPLC–MS

Jin

Song

et al. 2015

Chromatographia

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Isolation and evaluation of immunological adjuvant activities of saponins from the roots of Pulsatilla chinensis with less adverse reactions

Cited by 55 publications

References 51 publications

Fast separation of triterpenoid saponins using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

Fast separation of triterpenoid saponins using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

A polysaccharide isolated from Agaricus blazei Murill (ABP-AW1) as a potential Th1 immunity-stimulating adjuvant

Simultaneous Determination of Triterpenoidal Saponins in Rat Urine After Oral Administration of Pulsatilla chinensis Extract Using HPLC–MS

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