Isolation and Identification by Cytoprotection Assay of Antioxidative Compound Contained in Vinegar Produced from Sweet Potato-Shochu Post-Distillation Slurry

Kawano, Kuniaki; Morimura, Shigeru; Mori, Etsuro; Matsushita, Hirokazu; Ohta, Hiroto; Kida, Kenji

doi:10.3136/fstr.16.327

Cited by 9 publications

(4 citation statements)

References 19 publications

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“…) and fertilizer (Kawano et al . ). Shochu slop has also been tapped as an energy source to generate electricity and heat after conversion into biogas (Kanai et al .…”

Section: Introductionmentioning

confidence: 97%

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Shochu slop is an excellent medium forEscherichia coliK‐12

Suzuki

Nishida

Tamaki

2019

Lett Appl Microbiol

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We found that shochu slop, the residue generated during the production of distilled shochu liquor, which must be treated as industrial waste, can be used as an excellent medium for Escherichia coli culture. LB medium is generally used in laboratories for culturing E. coli. However, it is not the optimal medium for E. coli culture because the bacterial cells cannot grow to very high densities in LB medium. On the other hand, E. coli can grow to higher densities in Terrific broth and this medium is used when researchers want to grow E. coli to high density or to obtain a protein with high yield. In this study, we removed solid matter from shochu slop, adjusted the pH of the mixture to 7 and subsequently used the slop for E. coli culture. The ability of shochu slop to support E. coli growth was compared with those of LB Miller medium and Terrific broth. The results indicate that sweet potato shochu slop as culture medium for E. coli is comparable to Terrific broth and much better than LB Miller medium in terms of supporting cell proliferation, and plasmid and enzyme production. Significance and Impact of the Study Shochu manufacturers incur a cost to dispose shochu slop, which is recognized as food manufactural residues. Escherichia coli has been used in laboratories and in industry. However, culture media used in the laboratories are expensive and those used in industry are expensive because of their large scale. We found that sweet potato shochu slop is an excellent culture medium for E. coli. This finding is not only useful for laboratories and industry, but also beneficial to the effective utilization of this renewable resource to create a sustainable society.

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“…) and fertilizer (Kawano et al . ). Shochu slop has also been tapped as an energy source to generate electricity and heat after conversion into biogas (Kanai et al .…”

Section: Introductionmentioning

confidence: 97%

“…Several ways to utilize shochu slop have been developed. For instance, shochu slop is dehydrated and used as feedstuff (Mahfudz et al 1996) and fertilizer (Kawano et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Shochu slop is an excellent medium forEscherichia coliK‐12

Suzuki

Nishida

Tamaki

2019

Lett Appl Microbiol

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“…Phenolics play a critical role in keeping health-promoting. Vinegar has an antioxidant effect (Kawano et al, 2010;Yusoff et al, 2015). Different kinds of vinegars have different oxidation resistance because the active ingredients in them are different (Sugiyama et al, 2003;Kim et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Eucommia Ulmoides Leaves Vinegar Process to Improve the Antioxidant Activity

Chun-feng

Zhang

2019

FSTR

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Chlorogenic acid (CA) has strong oxidative resistance. The content of CA is high in Eucommia ulmoides leaves which are easily available and low cost. Therefore, in this work, using Eucommia ulmoides leaves enzymatic hydrolysate (EEH) as the main material, the fermentation conditions of Eucommia ulmoides leaves vinegar (EV) were studied to increase the antioxidant activity of the vinegar. Box-Behnken design indicated the following optimal fermentation conditions: EEH 48 %, acetic acid bacteria solution 10 %, ethanol addition 6 %. Under above conditions, the acetic acid content and CA content of EV was 41.5±2.2 mg/mL and 1.1±0.17 mg/mL, respectively. EV exhibited the higher antioxidant activity, with IC 50 values of 2.62 μg/ mL, 27.21 μg/mL and 1.72 mg/mL for DPPH, hydroxyl radical scavenging activity and reducing power, respectively. The addition of Eucommia ulmoides leaves increased the antioxidant activity of vinegar.

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“…On the other hand, it is important that the SDR do include various useful organic compounds derived from the fermentation of grains. 6) Therefore, there were some reports on biological and bioactive effects of SDR such as growth-stimulating effects on Bifidobacterium by sweet potato-SDR, 7) antioxidative cytoprotection activity of vinegars produced from rice-, barley, and sweet potato-SDR. 8) We succeeded in developing membrane-targeted chemotherapy with hybrid liposomes composed of phospholipid (L-α-dimyristoylphosphatidylcholine) and nonionic surfactants (polyoxyethylenelauryl ether) in vitro, in vivo, and in clinical applications.…”

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confidence: 99%

Anticancer Effects of Residual Powder from Barley-Shochu Distillation Remnants against the Orthotopic Xenograft Mouse Models of Hepatocellular Carcinoma in Vivo

Ohgidani

Ichihara

Goto

et al. 2012

Biological & Pharmaceutical Bulletin

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Barely-Shochu is a traditional Japanese liquor distilled from fermented barley with Saccharomyces cerevisiae. Barely-Shochu distillation remnants (SDR) are by-products in the manufacturing process of barley-Shochu. We have already reported on valuable powder from Shochu distillation remnants (PSDR) including antioxidative compounds such as polyphenols. In this study, we investigated the therapeutic effects of barely-PSDR against orthotopic xenograft mouse models of hepatocellular carcinoma (HCC) in vivo. We constructed a mouse model of HCC by orthotopical inoculation of HepG2 cells into the liver of SCID mice. Barely-PSDR (2250 mg/kg) was orally treated once each day for 21 d after the inoculation of HepG2 cells. The livers were removed from anaesthetized mice after the treatment with barely-PSDR and fixed in formalin. The liver sections were analyzed by hematoxylin and eosin (HE) staining and terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick-end labeling (TUNEL) methods. Remarkably high reduction of tumorigenesis was obtained in the mouse models of HCC after the oral administration of barely-PSDR in vivo. Induction of apoptosis in the liver section on the mouse models treated with barely-PSDR was observed. Furthermore, prolonged survival was obtained. Thus, therapeutic effects of barely-PSDR without side effects on the orthotopic xenograft mouse models were revealed for the first time.

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Isolation and Identification by Cytoprotection Assay of Antioxidative Compound Contained in Vinegar Produced from Sweet Potato-Shochu Post-Distillation Slurry

Cited by 9 publications

References 19 publications

Shochu slop is an excellent medium forEscherichia coliK‐12

Shochu slop is an excellent medium forEscherichia coliK‐12

Optimization of <i>Eucommia Ulmoides</i> Leaves Vinegar Process to Improve the Antioxidant Activity

Anticancer Effects of Residual Powder from Barley-<i>Shochu</i> Distillation Remnants against the Orthotopic Xenograft Mouse Models of Hepatocellular Carcinoma <i>in Vivo</i>

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