Isolation and identification new bacterial strains isolated from different sources of Al-Rafidiyah oil field in Iraq

Hamzah, Asaad Faraj; Abd-Alsahib, Wijdan Hussein; Mahdi, Saad S

doi:10.21608/cat.2020.23299.1041

Cited by 5 publications

(8 citation statements)

References 15 publications

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“…One strain of B. sonorensis species was isolated from one habitat in present study, this species showed high similarity with strains of B. licheniformis and all of them included in cluster III of phylogenetic tree. B. sonorensis was recorded as a distinct species for first time by Palmisano et al (2001), this species was isolated in previous study from the produced water of the Al-Rafidiyah oil field in Basrah province (Hamzah et al 2020). Two strains of B. safensis and one strain of B. pumilus were isolated from soil sample of B habitat, where both species formed one cluster in phylogenetic tree.…”

Section: Discussionmentioning

confidence: 92%

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Distribution, occurrence and molecular characterization of Bacillus related species isolated from different soil in Basrah Province, Iraq

ALYOUSIF

2022

Biodiversitas

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Abstract. Alyousif NA. 2021. Distribution, occurrence and molecular characterization of Bacillus related species isolated from different soil in Basrah Province, Iraq. Biodiversitas 23: 679-686. Bacillus related species are widely predominant in various environments such as soil with diverse environmental conditions. These bacteria are one of the most explored groups of bacteria in industrial biotechnology due to their enzymes. The present study was aimed to isolate, screen and determine the distribution of Bacillus related species from soil samples. The total Bacillus related species counts in one gram of each soil sample ranged between 3.2 X 103 cfu/g and 4 X 103 cfu/g. A total of 43 isolates with different morphologies were isolated from the soil samples out of ten different habitats and the number of morphotypes recovered from each habitat varied from 2 to 7. The molecular identification of isolates by PCR technique and sequencing of 16S rDNA gene indicated that 43 isolates belonged to the genera Bacillus, Cytobacillus, Priestia and Peribacillus. The species Bacillus licheniformis and Bacillus subtilis were the most distributed species in the habitats. The bacterial isolates were showed 100% similarity, except 8 isolates were showed 99% similarity were identified as new strains and their sequences were deposited at the National Center for Biotechnical Information (NCBI). The phylogenetic tree was constructed by MEGA X software based on 16S rDNA gene sequences of Bacillus related species to evaluate their close relationship and evolution. The study revealed the higher diversity and distribution of Bacillus related species in the soil samples and the molecular technique was the best method for identifying Bacillus related species.

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Section: Discussionmentioning

confidence: 92%

“…B. albus was recorded as novel species of the Bacillus cereus group by . Bacillus cereus strains were isolated from the soil samples of Basrah province in previous studies (Alyousif et al 2020;Hamzah et al 2020).…”

Section: Discussionmentioning

confidence: 99%

Distribution, occurrence and molecular characterization of Bacillus related species isolated from different soil in Basrah Province, Iraq

ALYOUSIF

2022

Biodiversitas

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“…Ozyurek and Bilkay (2017) who used an oil field as a microbial source, showed the same results, in their study, different Gram-negative and positive bacteria were isolated from different sources of oil fields. Many other studies have found that the majority of bacteria found in produced water samples were Gram-positive bacteria (Elumalai et al 2019;Hamzah et al 2020). The reason for Gram-positive dominance is that Gram-positive bacteria have strong cell wall, which allow them to adapt more easily to extreme conditions such as high temperature and osmotic pressur (Prakash et al 2014).…”

Section: Bacterial Isolationmentioning

confidence: 99%

“…This may explain why the bacterial load in separator tank water samples is relatively lower compared to pond water samples. Upon reviewing relevant publications on oil reservoirs, the best dilution for obtaining separate colonies from separator tank water was not stated, with the exception of Hamzah et al (2020), who revealed that 10 -2 was the ideal dilution. Regarding the pond water samples, there are no studies that have investigated their microbiology; they were sampled for the first time in the current study.…”

Section: Bacterial Isolationmentioning

confidence: 99%

Screening for extracellular synthesis of silver nanoparticles by bacteria isolated from Al-Halfaya oil field reservoirs in Missan province, Iraq

2022

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Abstract. Alshami HGA, Al-Tamimi WH, Hateet RR. 2022. Screening for extracellular synthesis of silver nanoparticles by bacteria isolated from Al-Halfaya oil field reservoirs in Missan province, Iraq. Biodiversitas 23: 3462-3470. Microorganisms that live in deep environments are thought to be adapted to the conditions of these environments and possess enzymatic systems that can be useful in scientific and commercial applications. The study aimed to isolate, identify, and screening of silver nanoparticles (AgNPs)-producing bacteria from five samples of produced water of the Al-Halfaya oil field reservoirs in Missan governorate, Iraq. The results revealed a total of 22 bacterial isolates were isolated and identified by morphological features and amplification of the 16S rDNA gene. The isolates are belonging to nine species, including Bacillus cereus strain DBA1.1, Bacillus thuringiensis strain MSP51, Neobacillus drentensis strain ROA042, Enterococcus faecalis strain 2674, Exiguobacterium mexicanum strain AB201, Klebsiella quasipneumoniae strain KP18-31, Klebsiella quasipneumoniae subsp. similipneumoniae strain 2437, Klebsiella pneumoniae strain IOB-L, and Acinetobacter lwoffii strain K34. The Gram-positive bacteria were the most dominant (55.56%), and the most frequent isolate was Klebsiella quasipneumoniae strain KP18-31 (63.64%). Six species out of nine species were recognized as AgNPs producing bacteria and B. cereus strain DBA1 was the best and most potent isolate in the synthesis of AgNPs. The phylogenetic tree was constructed depending on 16S rDNA gene sequences to determine the evolutionary relationship among the isolated bacteria.

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“…In this study, 21 diagnosed bacterial isolates were used [16] which were isolated from various oil sources. for screening of biopolymer production activity 2 ml of overnight broth culture of each isolates were add to 250 ml Erlenmeyer flask containing 100 ml of sterilized biopolymer producing media modification of [17] including (per litter distilled water) 40 g sucrose as a carbon source, 0.5 g NaNO3, 1.0 g yeast extract, 0.5 g KH2PO4, 0.25 g MgSO4•7H2O, pH 7.0.…”

Section: Screening Of Biopolymer Productionmentioning

confidence: 99%

Screening of extracellular polymeric substance producing bacteria isolated from environmental sources and testing their ability to seal fractures zones of oil reservoir rocks

Hamzah¹,

Al-Tamimi²

2022

Proceedings of 2nd International Multi-Disciplinary Conference Theme: Integrated Sciences and Technologies, IMDC-IST 2021, 7-9

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In this study, 21 environmental bacterial isolates isolated from different environmental sources in Basra Governorate identified phenotypically and genetically based on 16 rRNA gene sequence analysis were screened to reveal their ability to produce biopolymer based on the viscosity scale formed in the cultural media and use of Sudan Black stain. Six isolates showed their ability to produce biopolymer, and the two isolates of Bacillus licheniformis strain CC91, Bacillus thuringiensis strain LU3 recorded the best results. The isolate of the Bacillus licheniformis strain CC91 was tested in the process of sealing fractures and openings with high permeability for samples of oil reservoir rocks.

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Isolation and identification new bacterial strains isolated from different sources of Al-Rafidiyah oil field in Iraq

Cited by 5 publications

References 15 publications

Distribution, occurrence and molecular characterization of Bacillus related species isolated from different soil in Basrah Province, Iraq

Distribution, occurrence and molecular characterization of Bacillus related species isolated from different soil in Basrah Province, Iraq

Screening for extracellular synthesis of silver nanoparticles by bacteria isolated from Al-Halfaya oil field reservoirs in Missan province, Iraq

Screening of extracellular polymeric substance producing bacteria isolated from environmental sources and testing their ability to seal fractures zones of oil reservoir rocks

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