Isolation and Identification of Antibacterial Compound from Punica granatum L. Fruit Peels Grown in Iraq

Abstract

The aim of this study was to isolate and identify the substance responsible for the pharmacological activities of Punica granatum L. fruit peels. Tannic acid (ellagitannins, gallotannins) was isolated from pomegranate fruit husk and detected on Thin layer chromatography TLC plates in comparison with standard tannic acid that, served as a positive control. Moreover, infrared FTIR spectrometer and High Performance Liquid Chromatography HPLC analysis were used to confirm the purity and characterization of tannic acid. The isolated material was investigated for its antibacterial activity against six different pathogenic bacteria. The Gram-positive bacteria were; Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes; and Gram-negative bacteria included; Brucella melitensis, Klebsiella pneumoniae and Pseudomonas aeruginosa. Tannic acid at different concentrations (50.0, 25.0, 12.5, 6.2 mg/ml) was active against all tested bacteria and the highest inhibitory effect was observed against K. pneumoniae (zone of inhibition: 22.6 mm) using the disc diffusion method. The minimal inhibitory concentration MIC for tannic acid was determined using a broth microdilution method in 96 multi-well microtiter plates. MIC values ranged from 31.2-250.0 µg/ml, and the most promising result was observed against K. pneumoniae.

BACTERIAL INHIBITORY EFFECT OF ALGERIAN POMEGRANATE (Punica Granatum L.) EXTRACTS (PEEL, JUICE, AND SEED) AGAINST MULTIDRUG RESISTANT BACTERIA

“…It was interesting to note that the most remarkable and maximum values were illustrious for ethanolic and acetonic peel extracts against Leclercia adecarboxylata, Proteus vulgaris, Citrobacter diversus, and Escherichia coli (E. coli) with inhibition zones higher than 26 mm and MIC value < to 32 µg/mL. These results are in total agreement with other studies, such as the Tunisian variety of P.granatum (Hayouni et al, 2011), the Iraqi variety (Taha Mahmood et al, 2010;Ahmed, 2016) and the Indian variety (Barathikannan et al, 2016). Our MICs value are higher than those reported by Gullon et al, (2016) for Egyptian variety, MICs values obtained in this study were between 20 and 50 mg/mL).In fact, the inhibition zones produced by P. granatum extracts were more or less comparable to the pure antibiotic compounds taken as positive drug controls, albeit at lesser concentrations (Fig.…”

In vitro Antioxidant, Antimicrobial and in vivo Peripheral Analgesic Activities of Methanol and Petroleum Ether Extracts of Whole Plant of Uraria lagopoides DC

“…However antimicrobial effect of the alkaloid is thought to be through a different mechanism as the compound inhibits topoisomerase to intercalate DNA and to inhibit DNA synthesis [41][42][43]. The tannin contents of the plant extracts may also contribute to the antimicrobial activity [44][45][46][47][48]. The antimicrobial mechanisms of tannin might be due to their astringent property that can induce complexation with microbial enzymes or substrates, iron deprivation, hydrogen bonding or nonspecific interaction with microbial enzymes, toxic action on microbial membranes, complexation of metal ions [49][50][51].…”