Isolation and Identification of Cellulose Degrading Bacteria from Mangrove Soil of Mahanadi River Delta and Their Cellulase Production Ability

Behera, Bikash Chandra; Parida, S.; Dutta, Sushil Kumar; Thatoi, H. N.

doi:10.12691/ajmr-2-1-6

Cited by 96 publications

(94 citation statements)

References 17 publications

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“…Behera et al (2014) have also reported similar trend in cellulase production using Bacillus spp. The decrease in cellulolytic activity after 48 h might be due to either depletion of nutrients in the medium.…”

Section: Discussionsupporting

confidence: 62%

Exploitation of Agro-Industrial Wastes as Substrates for Cellulase Production by Bacillus licheniformis MTCC 429

Kumaran¹,

Kalaichelv²,

Santhi³

2015

Microbiology J.

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The aim of the present investigation was to exploit the cheap agro-waste, sugarcane bagasse as a substrate for cellulase enzyme production using Bacillus licheniformis MTCC 429. The cellulase producing ability of B. licheniformis MTCC 429 was assessed using CMC agar plates. The factors affecting cellulase production were optimized by varying the parameters of incubation period, temperature and pH. The maximum cellulase enzyme production was achieved when the production medium pH was maintained at pH 7.0, temperature of 35°C with an incubation time of 48 h. Among the different concentration of sugarcane bagasse hydrolysate tested, CMC medium amended with 5% seemed to exhibit maximum cellulase productivity. Further, the partially purified enzyme was tested for its pH and temperature stability. The properties presented by B. licheniformis MTCC 429 suggest that the strain showed enhanced production of lignocellulosedegrading cellulase enzyme under the optimized conditions. The temperature and pH stability of the enzyme suggested its potential for industrial applications involving elevated conditions.

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Section: Discussionsupporting

confidence: 62%

Exploitation of Agro-Industrial Wastes as Substrates for Cellulase Production by Bacillus licheniformis MTCC 429

Kumaran¹,

Kalaichelv²,

Santhi³

2015

Microbiology J.

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“…Lignin: The residue that remained after determination of cellulose was treated with 25 ml KMnO 4 buffer for 90 min at [20][21][22][23][24][25] o C. Lignin was dissolved leaving cutin and silica as insoluble materials. The contents were then filtered through tarred sintered crucible using gentle suction and residue was washed with distilled water, then with acetone.…”

Section: Hemicellulose (%) = Ndf (%)-Adf (%)mentioning

confidence: 99%

“…The CMC agar comprising (g/L) CMC (SigmaAldrich, Germany), 5; NaNO 3 (Lab M, India), 1; K 2 HPO 4 (Applichem, Germany), 1; KCl (Lab M, UK), 1; MgSO 4 (Sigma-Aldrich, Germany), 0.5; yeast extract (Sigma-Aldrich, Germany), 0.5; glucose (Oxoid, UK), 1 and Agar (Sigma-Aldrich, Germany), 17) was prepared by dissolving the ingredients in 1 L distilled water. The mixture was heated to a boil to homogenize the sample and sterilized in an autoclave at 121 After 48 h of incubation, each of the duplicate plates was screened for cellulase activity by flooding the plates with 0.1% Congo red (SigmaAldrich, Germany) solution and left undisturbed for 15-20 min and then destained with 1 M NaCl (Oxoid, UK) [21]. Halo zones around the growing cellulolytic bacteria confirmed positive isolates.…”

Section: Isolation and Screening Of Cellulolytic Bacteriamentioning

confidence: 99%

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Bioethanol Production by an Ethanol-Tolerant Bacillus cereus Strain GBPS9 Using Sugarcane Bagasse and Cassava Peels as Feedstocks

Ezebuiro¹,

Ogugbue²,

Oruwari³

et al. 2015

J Biotechnol Biomater

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Bioethanol production potential of ethanol-tolerant Bacillus cereus strain GBPS9 using sugarcane bagasse and cassava peels as feedstocks was investigated. The Bacillus cereus GBPS9 used in this study was isolated from agro-wastes impacted soil and classified based on phylogenetic analysis of its 16S rRNA gene. The sequence of the isolate has been deposited in GenBank under the accession number KT318371.1. The isolate was selected based on its cellulolytic ability, tolerance to ethanol concentration of 6% (v/v) and ability to ferment sugar to ethanol. The substrates employed in the study were cassava peels and sugarcane bagasse. Chemical composition analysis showed total carbohydrate and lignin contents (% dry weight) of 69.6 ± 1.2 and 13.9 ± 0.4 for cassava peels and 70.3 ± 1.9 and 16.2 ± 1.2 for sugarcane bagasse, respectively. The feedstocks were subjected to acid, alkali and steam explosion pretreatments to increase cellulose content and therefore, reduce lignin content. The best pretreatment methods (steam explosion for sugarcane bagasse and acid for cassava peels) increased total carbohydrate contents to 85.4 ± 2.33 and 80.4 ± 2.5 for sugarcane bagasse and cassava peels, respectively. The respective lignin contents after pretreatment were 4.2 ± 0.44 and 4.8 ± 0.8 for sugarcane bagasse and cassava peels. Cultural conditions (pH, temperature, nitrogen source, inoculum size and substrate concentration) of the bacterium were optimized to enhance cellulase production. The laboratory scale fermentation of the feedstocks to ethanol was carried out in 250 mL Erlenmeyer flasks. Gas Chromatography -Mass spectrometry (GC-MS) analysis of the fermentation broth of sugarcane bagasse and cassava peels substrates revealed ethanol contents of 18.40 and 17.80 g/L, respectively. The study has demonstrated efficient bioethanol production by Bacillus cereus GBPS9 using sugarcane bagasse and cassava peels as feedstocks.

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“…Some strains are harmful to humans and cause food borne illnesses, while others have beneficial applications, such as probiotics for animals. There are reports on the production of cellulase by B. cereus strains and on their potential for the production of bioethanol (Yan et al 2011;Behera et al 2014). …”

Section: Isolation Screening and Identification Of The Cellulase-prmentioning

confidence: 99%

Bioethanol Production with Cellulase Enzyme from Bacillus cereus Isolated from Sesame Seed Residue from the Jazan Region

et al. 2018

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Bio-ethanol is considered as an important renewable fuel to partly replace fossil-derived fuels. In this study, bioethanol production, which includes cellulase production, saccharification of the cellulose content of sesame seed residue, and ethanol production, was investigated. Out of the hundreds of cellulase-producing bacterial strains isolated from sesame seed residue during this study, the B isolate was found to have the highest cellulase enzyme production. This isolate was identified as Bacillus cereus by 16S rRNA sequencing. The effects of different growth parameters, including inoculum concentration, incubation time, temperature, pH, and carbon and nitrogen sources, were investigated to optimize the growth conditions of the bacterifum. The maximum cellulase activity was achieved with an inoculum concentration of 3% after 48 h in a basal medium at a pH of 7 and an incubation temperature of 35 °C. The best nitrogen and carbon sources were yeast extract and sesame seed residue, respectively. The results showed the liberation of 2.3 g/L of reducing sugar by the dinitrosalicylic acid method. This total reducing sugar produced 15 g/L of ethanol after 48 h when Saccharomyces cerevisiae was used as a fermentation agent. Hence, bioethanol was successfully produced from the cellulose of sesame seed residue using the cellulase enzyme from B. cereus.

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Isolation and Identification of Cellulose Degrading Bacteria from Mangrove Soil of Mahanadi River Delta and Their Cellulase Production Ability

Cited by 96 publications

References 17 publications

Exploitation of Agro-Industrial Wastes as Substrates for Cellulase Production by Bacillus licheniformis MTCC 429

Exploitation of Agro-Industrial Wastes as Substrates for Cellulase Production by Bacillus licheniformis MTCC 429

Bioethanol Production by an Ethanol-Tolerant Bacillus cereus Strain GBPS9 Using Sugarcane Bagasse and Cassava Peels as Feedstocks

Bioethanol Production with Cellulase Enzyme from Bacillus cereus Isolated from Sesame Seed Residue from the Jazan Region

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