Isolation and identification of chitin in three-dimensional skeleton of Aplysina fistularis marine sponge

Wysokowski, Marcin; Bazhenov, Vasilii V.; Tsurkan, Mikhail V.; Galli, Roberta; Stelling, Allison L.; Stöcker, Hartmut; Kaiser, Sabine; Niederschlag, Elke; Gärtner, G.; Behm, Thomas; Ilan, Micha; Petrenko, Alexander; Jesionowski, Teofil; Ehrlich, Hermann

doi:10.1016/j.ijbiomac.2013.08.039

Cited by 94 publications

(64 citation statements)

References 31 publications

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“…As a preliminary procedure for identifying the intactness of chitin structures in these composites we used a specific chitin stain Calcofluor White (CFW), the fluorescent brightener [26,27,[39][40][41][42][43][44][45][46]. CFW binds to the glucan chains and linear β-(1,4)-glucosidically linked units of N-acetyl-D-glucosamine.…”

Section: Resultsmentioning

confidence: 99%

“…The thermal stability of chitin under hydrothermal conditions and the presence of various functional groups (-OH and C=O and -NH) in the chitin molecule permit the utilization of this biopolymer in hydrothermal synthesis reactions. Our previously published experimental results showed strong evidence [5][6][7][8][9] that this is a key way to use chitinous matrices of sponge origin as a nanostructured biological scaffold [26][27][28]. A broad variety of reactions related to hydrothermal synthesis can be performed in vitro.…”

Section: Research Article Open Accessmentioning

confidence: 95%

“…S8045) were obtained from Sigma-Aldrich (Germany). The α-chitin standard from A. aerophoba was prepared according to a previously described method [27]. In brief, isolation was performed in three basic steps: (i) removal of water soluble salts and impurities by washing with distilled water; (ii) removal of proteins and residual pigments by treatment with 2.5 M NaOH solution; (iii) removal of calcium and magnesium carbonates by treatment with 20% acetic acid.…”

Section: Methodsmentioning

confidence: 99%

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Renewable chitin from marine sponge as a thermostable biological template for hydrothermal synthesis of hematite nanospheres using principles of extreme biomimetics

Wysokowski

Petrenko

Motylenko

et al. 2015

Bioinspired Materials

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range of uses. Here, chitin-based scaffolds isolated from the skeleton of marine demosponge Aplysina aerophoba were used as a template for the in vitro formation of iron oxide from a saturated iron(III) chloride solution, under hydrothermal conditions (pH~1.5, 90 °C). The resultant chitin-Fe 2 O 3 three dimensional composites, prepared for the first time via hydrothermal synthesis route, were thoroughly characterized using light, fluorescence and scanning electron microscopy; as well as with analytical methods like Raman spectroscopy, electron diffraction and HR-TEM. The results show that this versatile method allows for efficient chitin mineralization with respect to hematite. Additionally, we demonstrate that chitin nanofibers template the nucleation of uniform Fe 2 O 3 nanocrystals.

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Section: Resultsmentioning

confidence: 99%

Section: Research Article Open Accessmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Renewable chitin from marine sponge as a thermostable biological template for hydrothermal synthesis of hematite nanospheres using principles of extreme biomimetics

Wysokowski

Petrenko

Motylenko

et al. 2015

Bioinspired Materials

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“…The biomimetic potential of chitin-based composite biomaterials of poriferan origin is analyzed in detail in a recently published monograph entitled "Biomimetic biomaterials: structure and applications" [57]. Furthermore, we show how sponge chitin can be used as a thermostable template for developing a new generation of biocomposites using "extreme biomimetic conditions" via a hydrothermal synthesis route (see for details [114,137,[159][160][161]).…”

Section: Discussionmentioning

confidence: 97%

Poriferan Chitin as a Versatile Template for Extreme Biomimetics

et al. 2015

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Abstract:In this mini-review, we shall first cover a short history of the discovery of chitin isolated from sponges; as well as its evolutionarily ancient roots. Next, we will delve into the unique structural, mechanical, and thermal properties of this naturally occurring polymer to illuminate how its physicochemical properties may find uses in diverse areas of the material sciences. We show how the unique properties and morphology of sponge chitin renders it quite useful for the new route of "Extreme Biomimetics"; where high temperatures and pressures allow a range of interesting bioinorganic composite materials to be made. These new biomaterials have electrical, chemical, and material properties that have applications in water filtration, medicine, catalysis, and biosensing. OPEN ACCESSPolymers 2015, 7 236

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“…This study is concerned with chitosan (CS) biopolymer, a linear polysaccharide that can be derived from the alkaline N-deacetylation of chitin [4,5]. Chitin is the main structural component of the shells of crustaceans, the exoskeletons of insects and the cell walls of fungi [4][5][6][7][8]. CS polysaccharide is a copolymer comprising β-(1-4)-2-amino-D-glucose (deacetylated unit) and β-(1-4)-2-acetamido-D-glucose (acetylated unit) [4,5].…”

Section: Introductionmentioning

confidence: 99%

A Comparative Analysis of the Reinforcing Efficiency of Silsesquioxane Nanoparticles versus Apatite Nanoparticles in Chitosan Biocomposite Fibres

Wang

Pasbakhsh²,

Silva

et al. 2017

J. Compos. Sci.

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A comparative analysis of the effects of polyhedral oligomeric silsesquioxane (POSS) and hydroxyapatite (HA) for reinforcing chitosan (CS) is given here. Wet-spun CS nanocomposite fibres, blended with HA or POSS nanoparticles, at varying concentrations ranging from 1 to 9% (w/w) were stretched until rupture to determine the mechanical properties related to the elasticity (yield strength and strain, stiffness, resilience energy) and fracture (fracture strength strain and toughness) of the composite. Two-factor analysis of variance of the data concluded that only the fracture-related properties were sensitive to interaction effects between the particle type and concentration. When particle type is considered, the stiffness and yield strength of CS/POSS fibres are higher than CS/HA fibres-the converse holds for yield strain, extensibility and fracture toughness. With regards to sensitivity to particle concentration, stiffness and yield strength reveal trending increase to a peak value (the optimal particle concentration associated with the critical aggregation) and trending decrease thereafter, with increasing particle concentration. Although fracture strength, strain at fracture and fracture toughness are also sensitive to particle concentration, no apparent trending increase/decrease is sustained over the particle concentration range investigated here. This simple study provides further understanding into the mechanics of particle-reinforced composites-the insights derived here concerning the optimized mechanical properties of chitosan composite fibre may be further developed to permit us to tune the mechanical properties to suit the biomedical engineering application.

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Isolation and identification of chitin in three-dimensional skeleton of Aplysina fistularis marine sponge

Cited by 94 publications

References 31 publications

Renewable chitin from marine sponge as a thermostable biological template for hydrothermal synthesis of hematite nanospheres using principles of extreme biomimetics

Renewable chitin from marine sponge as a thermostable biological template for hydrothermal synthesis of hematite nanospheres using principles of extreme biomimetics

Poriferan Chitin as a Versatile Template for Extreme Biomimetics

A Comparative Analysis of the Reinforcing Efficiency of Silsesquioxane Nanoparticles versus Apatite Nanoparticles in Chitosan Biocomposite Fibres

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