Isolation and Identification of Potential Probiotic Bacteria from Cattle Farm Soil in Dibrugarh District

Abstract

Several studies have been done to isolate probiotic bacteria from different sources. In this present study, an attempt was made to isolate, screen and identify potential probiotic bacteria from cattle farm soil in Dibrugarh district, Assam, India. At the level of screening, the result showed the isolates designated as DUA4, DUD3 and DUE2 showed a percent survival rate of 75.36, 69.14 and 52.36 respectively at a pH of 2.5. Similarly survival rate of the same isolates in 0.5% bile salt condition was found to be 117.17%, 144.59% and 118.10% for the isolates DUA4, DUD3 and DUE2 respectively. Antimicrobial activity of the isolates towards the indicator organisms tested showed that DUA4 inhibited gram positive organisms while DUD3 showed activity against both gram positive and gram negative bacteria. All the three isolates showed activity against L. monocytogenes. Autoaggregation ability of the isolates DUA4, DUD3 and DUE2 was found to be 44.15%, 54.11% and 9.42% respectively. The adhesion ability of the isolates DUD3, DUA4 and DUE2 to xylene was 61.78%, 45.37% and 14.83% respectively. Antimicrobial susceptibility test of the isolates showed that the isolates are in general sensitive to antibiotics tested. Phenotypic and genotypic analysis based on the 16S rRNA gene sequence of the isolates DUA4, DUD3 and DUE2 resulted in the identification and designation of the isolate DUA4 as Bacillus spp., DUD3 as Enterococcus faecium and DUE2 as Enterobacter sp. In conclusion, the study has indicated the possibility of isolating potential probiotic bacterial strains from cattle farm soil.

Characterization of a Newly Isolated Probiotic Strain from Oecophylla smaragdina, an Edible Insect Popular Among the Indigenous Communities of Northeast India

“…It is universally recognized that probiotics must be able to colonize the digestive tract to adhere and colonize the intestine apart from being resistant to GI condition which is considered as a crucial factor for the selection of new probiotics [24][25][26]. The studied bacterial isolate shows [38] concurrent or even higher percentages of hydrophobicity (46.2 ± 0.32%) and auto-aggregation (61.41 ± 0.28%) compared to other reported probiotic isolates (Table 5) [10,27,28]. The comparison table shows S. succinus with highest value of hydrophobicity (69.11%) but shows poor antimicrobial potential as compared to the current isolate Bacillus sp.…”

Isolation and Characterization of Human Intestinal Bacteria Cytobacillus oceanisediminis NB2 for Probiotic Potential

“…Recently, probiotic bacteria are being extensively explored and characterized for their potential therapeutics for various human disorders (Yadav and Chauhan, 2022 ). Probiotic strains were identified from various sources like dairy products (Haghshenas et al, 2017 ; Karami et al, 2017 ), fermented drinks (Angelescu et al, 2019 ; Setta et al, 2020 ), plants (Rahman et al, 2018 ; Samedi and Charles, 2019 ), soil (Siraj et al, 2017 ), and animals (Abdou et al, 2018 ; Li et al, 2021 ). Human gut microbiota are being extensively characterized for their health-promoting benefits; however the full potential for their usage as microbiome therapeutics has humongous possibilities (Yadav and Chauhan, 2022 ).…”

On the use of probiotics to improve dairy cattle health and productivity

“…Our results compare favourably with the literature17 .More work is needed to better understand the exact mode of action of the probiotic product tested. The successful identification of inflammatory modulators (pro/pre), antibacterial peptides and development of a new biological mastitis therapy could significantly reduce the substantial economic losses incurred by the dairy industry worldwide and improve animal health, productivity and welfare while increasing food safety23 .…”

“…The current known mechanisms of action of probiotics in ruminants appears to be through a shift in the microbiota of rumen and rear-gut (small and large intestine), an improvement in fermentation or volatile fatty acids, the stabilisation of rumen pH, and improvements in the intestinal mucosal barriers through the probiotics competitively excluding pathogens and improving the local and systemic immune response28,29 . Probiotic bacteria have also been isolated from soil23 , fermented green tea30 , the gastro-intestinal tract of various animals including poultry31 and cattle32 . The most common genera of bacteria used include Lactobacillus spp., Bacillus spp., Bifidobacterium spp., Streptococcus spp.…”