Isolation and identification of Staphylococcus sp. in powdered infant milk

Palilu, Prayolga Toban; Budiarso, Tri Yahya

doi:10.1063/1.4983427

Cited by 7 publications

(7 citation statements)

References 6 publications

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“…Possible contamination occurs due to bulk tanks, less hygienic milking processes, and udders of dirty animals. In this study, milk samples were taken from previous research including pasteurized milk, 16 vendor and café milk, 17 powdered infant formula products, 18 and sweetened condensed milk. 19 All isolates of S. aureus produced staphylococcal enterotoxin A.…”

Section: Resultsmentioning

confidence: 99%

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The effect of temperature and Pasteurization time on Staphylococcus aureus isolates from dairy products

Yaniarti

Amarantini

Budiarso

2017

AIP Conference Proceedings

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Section: Resultsmentioning

confidence: 99%

“…aureus isolated from pasteurized milk, street vendor and café milk, powdered infant formula, and sweetened condensed milk products were collected by previous research from locations in Yogyakarta. [16][17][18][19] …”

Section: Methodsmentioning

confidence: 99%

The effect of temperature and Pasteurization time on Staphylococcus aureus isolates from dairy products

Yaniarti

Amarantini

Budiarso

2017

AIP Conference Proceedings

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“…This bacterium can grow in the range of pH values 4.2 -9.3. Staphylococcus sp is a pathogenic bacterium that has the ability to produce enterotoxins which can be a disease caused by consuming contaminated food (Pailu and Budiarso, 2017). Enterotoxins are enzymes that can survive in hot conditions and are resistant in an alkaline atmosphere in the intestine which can cause food poisoning (Rahayu et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

The Isolation of Staphylococcus Epidermidis Bacteria in White Snapper Salted Fish (Lates Calcalifer) of Sibolga City, North Sumatera Province

et al. 2020

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Salted fish is vulnerable to contamination by microbial. Staphylococcus epidermidis is one of the bacteria that can contaminate the salted fish. This research aims to isolate the Staphylococcus epidermidis bacteria contamination in salted white snapper fish sold in Sibolga City, North Sumatra Province. The sample used was white snapper salted fish, amounting to 10 samples from 10 traders. The isolation of Staphylococcus epidermidis was carried out using the Carter method. White snapper salted fish are mashed using a blender, then planted on Nutrient Broth (NB) as a bacterial growth media. Furthermore, identification of bacterial colonies grew using gram staining, Manitol Salt Agar (MSA) media, Blood Agar Plate (BAP) media, catalase test and confectionery media (Manitol and Glucose). The data obtained were analyzed descriptively. The results of this study suggest that Staphylococcus epidermidis contains bacterial contamination. Based on the data collected, it can be concluded that 7 out of 10 samples of white snapper salted fish sold in Sibolga City, North Sumatra Province, are contaminated with 70 percent Staphylococcus epidermidis bacteria.

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“…Tellurite forming colonies are reduced by the presence of salt and potassium tellurite in the media yolk (Ribeiro de Souza da Cunha, 2018). All samples except for P5 (UMS) that had black, shiny colonies in BPA media were steaked onto BHI plates to provide enrichment for the bacterial culture (Palilu & Budiarso, 2017). BHI media provided a nutrient-rich environment for Streptococci which is commonly challenging to grow.…”

Section: Isolation and Identification Of S Aureus And E Coli From Rec...mentioning

confidence: 99%

IDENTIFICATION AND OCCURRENCE OF ANTIBIOTIC RESISTANCE OF Staphylococcus aureus AND Escherichia coli ISOLATED FROM RECREATIONAL PARKS AROUND KOTA KINABALU, SABAH

Sugumaran

Jocksing

Yusof

2020

BIJB

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Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) are contributors to infection cases among the Asian population. S. aureus is found in the mucous lining of noses and is mainly non-pathogenic while E. coli, mostly harmless bacteria, are found in the intestine. Pathogenic strains of both bacteria have adverse effects on the elderly and younger age group of the population. Samples were collected from recreational parks around Kota Kinabalu as they are hotspots frequently visited by families with both age groups. The bacterial samples were isolated and cultured on selective media such as Baird-Parker agar (BPA), Brain Heart Infusion (BHI) agar, MacConkey agar and Eosin-Methylene Blue (EMB) agar. Morphological characteristics of bacterial growth were observed, where S. aureus had black-shiny growth in BPAand E. coli had a metallic-green sheen in EMB agar. The suspected bacteria samples were then stained and viewed under a light microscope. S. aureus was identified as gram-positive, stained violet with a circular shape and clustered appearance. E. coli was identified as gram-negative, stained red, rod-shaped with 2 – 3 bacterial alignment. Antibiotic resistance test resulted in S. aureus and E. coli samples did not display 100% resistance among 4 antibiotics tested (ampicillin, penicillin, tetracycline and chloramphenicol). Most of the bacteria samples were a minimum inhibitory of 0.1 mg/mL of antibiotic concentration. These results provide a foundation for further research on identifying bacterial strains using molecular methods. The findings can then be used to disseminate information to the public to create awareness of potential disease outbreaks in the city.

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Isolation and identification of Staphylococcus sp. in powdered infant milk

Cited by 7 publications

References 6 publications

The effect of temperature and Pasteurization time on Staphylococcus aureus isolates from dairy products

The effect of temperature and Pasteurization time on Staphylococcus aureus isolates from dairy products

The Isolation of Staphylococcus Epidermidis Bacteria in White Snapper Salted Fish (Lates Calcalifer) of Sibolga City, North Sumatera Province

IDENTIFICATION AND OCCURRENCE OF ANTIBIOTIC RESISTANCE OF Staphylococcus aureus AND Escherichia coli ISOLATED FROM RECREATIONAL PARKS AROUND KOTA KINABALU, SABAH

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