Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea

Ji, Chengyuan; Zhang, Yao; Sun, Ruini; Ma, Jiale; Pan, Zihao; Yao, Huochun

doi:10.3390/v13112217

Cited by 6 publications

(3 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The growth curve of the 10th passage virus shows that peak titer is reached at 30 hpi. By electron microscopy, the CHN-SCMY2021 particle is about 25 nm diameter which is consistent with the size of enterovirus particles ( Ji et al., 2021 ). Previous studies have shown that EV-G can be propagated in ST and Marc-145 cells ( Anbalagan et al., 2014 ; Mi et al., 2021 ; Shang et al., 2017 ).…”

Section: Discussionsupporting

confidence: 75%

Characterization, phylogenetic analysis, and pathogenicity of a novel genotype 2 porcine Enterovirus G

Xiao

Zhang

et al. 2023

Virus Research

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 75%

Characterization, phylogenetic analysis, and pathogenicity of a novel genotype 2 porcine Enterovirus G

Xiao

Zhang

et al. 2023

Virus Research

View full text Add to dashboard Cite

“…We utilized lentivirus packaging of the plasmid constructs since preliminary experiments with MDBK cells (Institute of Cell Biology, Chinese Academy of Sciences, Shanghai, China) [ 13 ] had indicated that Lipofectamine 2000 (Invitrogen) transfection efficiencies were <5%. We therefore co-transfected 293T cells (Shanghai Biological Technology Co., Ltd. Enzymere, Shanghai, China) with the lentivirus packaging plasmid psPAX2 and lentiviral vector pMD2.G at 2:1:1) that were then incubated for 5 h according to the manufacturer’s specifications (Omega Bio-Tek, Dallas, Texas, USA).…”

Section: Methodsmentioning

confidence: 99%

The MyoD1 Promoted Muscle Differentiation and Generation by Activating CCND2 in Guanling Cattle

Zhou¹,

Wang²,

Yang³

et al. 2022

Animals

View full text Add to dashboard Cite

The purpose of this study was to analyze the transcriptome of MyoD1 gene knockout MDBK cells (bovine kidney cells) using high-throughput sequencing. For the first time, CRISPR/CAS9 technology was used to construct a MyoD1 knockout in MDBK cells and transcriptome sequence analysis was used to examine MyoD1-related target gene expression. Transcriptome sequencing indicated the presence of 723 differentially expressed genes (DEGs) by comparing wild type and MyoD1 knockout MDBK cells and included 178 upregulated and 72 downregulated genes. The DEGs are mainly enriched in Pl-3-kinase and AKT, p53 signaling pathways. Quantitative RT-PCR confirmed that PDE1B, ADAMTS1, DPT, and CCND2 were highly expressed in the leg muscle, longissimus dorsi, and shoulder of Guanling cattle, and CCND2 was inhibited after MyoD1 knockout, suggesting it may be a key downstream gene of MyoD1 and associated with muscle formation and differentiation in Guanling cattle. This provides experimental data for subsequent studies on the regulatory mechanisms of muscle differentiation in Guanling cattle.

show abstract

“…Currently, the genus of Enterovirus contains 12 species of enteroviruses (A-L) and 3 species of rhinoviruses (A, B, C) [ 16 ]. Out of 12 enterovirus species, enterovirus E (EV-E), enterovirus F (EV-F), and enterovirus G (EV-G) are mainly related to the infections affecting the livestock industry [ 17 , 18 , 19 , 20 ]. While enterovirus infections have been increasingly reported in cattle and pigs, they are still largely unknown in small ruminants such as sheep or goats.…”

Section: Introductionmentioning

confidence: 99%

Unveiling of the Co-Infection of Peste des Petits Ruminants Virus and Caprine Enterovirus in Goat Herds with Severe Diarrhea in China

Zhang,

Zheng,

Zhang

et al. 2024

Viruses

View full text Add to dashboard Cite

Here, we report the discovery of two viruses associated with a disease characterized by severe diarrhea on a large-scale goat farm in Jilin province. Electron Microscopy observations revealed two kinds of virus particles with the sizes of 150–210 nm and 20–30 nm, respectively. Detection of 276 fecal specimens from the diseased herds showed the extensive infection of peste des petits ruminants virus (63.77%, 176/276) and caprine enterovirus (76.81%, 212/276), with a co-infection rate of 57.97% (160/276). These results were partially validated with RT-PCR, where all five PPRV-positive and CEV-positive specimens yielded the expected size of fragments, respectively, while no fragments were amplified from PPRV-negative and CEV-negative specimens. Moreover, corresponding PPRV and CEV fragments were amplified in PPRV and CEV double-positive specimens. Histopathological examinations revealed severe microscopic lesions such as degeneration, necrosis, and detachment of epithelial cells in the bronchioles and intestine. An immunohistochemistry assay detected PPRV antigens in bronchioles, cartilage tissue, intestine, and lymph nodes. Simultaneously, caprine enterovirus antigens were detected in lung, kidney, and intestinal tissues from the goats infected by the peste des petits ruminants virus. These results demonstrated the co-infection of peste des petits ruminants virus with caprine enterovirus in goats, revealing the tissue tropism for these two viruses, thus laying a basis for the future diagnosis, prevention, and epidemiological survey for these two virus infections.

show abstract

Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea

Cited by 6 publications

References 26 publications

Characterization, phylogenetic analysis, and pathogenicity of a novel genotype 2 porcine Enterovirus G

Characterization, phylogenetic analysis, and pathogenicity of a novel genotype 2 porcine Enterovirus G

The MyoD1 Promoted Muscle Differentiation and Generation by Activating CCND2 in Guanling Cattle

Unveiling of the Co-Infection of Peste des Petits Ruminants Virus and Caprine Enterovirus in Goat Herds with Severe Diarrhea in China

Contact Info

Product

Resources

About