Isolation and molecular characterization of multidrug-resistant halophilic bacteria from shrimp farm effluents of Parangipettai coastal waters

Sundaramanickam, Arumugam; Kumar, Poominathan Suresh; Kumaresan, S.; Balasubramanian, T.

doi:10.1007/s11356-015-4427-5

Cited by 23 publications

(7 citation statements)

References 29 publications

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“…More important, previous work on Bacillus flexus have shown that members of this species can degrade organic [25] and inorganic [26] nitrogen, thus making it a possible candidate for bioremediation in alkaline wastewater [27]. Some strains of B. flexus also demonstrate strong phosphorus solubilization activity [28], and others demonstrated resistance to OTC [29].…”

Section: Discussionmentioning

confidence: 99%

Agricultural Risk Factors Influence Microbial Ecology in Honghu Lake

Han

Dsouza

Zhou

et al. 2019

Genomics, Proteomics &Amp; Bioinformatics

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Agricultural activities, including stock-farming, planting industry, and fish aquaculture, can affect the physicochemical and biological characters of freshwater lakes. However, the effects of pollution producing by agricultural activities on microbial ecosystem of lakes remain unclear. Hence, in this work, we selected Honghu Lake as a typical lake that is influenced by agriculture activities . We collected water and sediment samples from 18 sites, which span a wide range of areas from impacted and less-impacted areas. We performed a geospatial analysis on the composition of microbial communities associated with physicochemical properties and antibiotic pollution of samples. The co-occurrence networks of water and sediment were also built and analyzed. Our results showed that the microbial communities of impacted and less-impacted samples of water were largely driven by the concentrations of TN, TP, NO 3 − -N, and NO 2 − -N, while those of sediment were affected by the concentrations of Sed-OM and Sed-TN. Antibiotics have also played important roles in shaping these microbial communities: the concentrations of oxytetracycline and tetracycline clearly reflected the variance in taxonomic diversity and predicted functional diversity between impacted and less-impacted sites in water and sediment samples, respectively. Furthermore, for samples from both water and sediment, large differences of network topology structures between impacted and less-impacted were also observed. Our results provide compelling evidence that the microbial community can be used as a sentinel of eutrophication and antibiotics pollution risk associated with agricultural activity; and that proper monitoring of this environment is vital to maintain a sustainable environment in Honghu Lake.

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Section: Discussionmentioning

confidence: 99%

Agricultural Risk Factors Influence Microbial Ecology in Honghu Lake

Han

Dsouza

Zhou

et al. 2019

Genomics, Proteomics &Amp; Bioinformatics

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show abstract

“…More important, previous work on Bacillus flexus have shown that members of this species can degrade organic(Guo et al 2013) and inorganic(Divyashree et al 2009) nitrogen, thus making it a possible candidate for bioremediation in alkaline wastewater(Wang and Zhao 2013). Some strains of B. flexus also demonstrate strong phosphorus solubilization activity(Gechemba et al 2015), and others demonstrated resistance to OTC(Sundaramanickam et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Agricultural Pollution Risks Influence Microbial Ecology in Honghu Lake

Han

Dsouza

Zhou

et al. 2018

Preprint

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BackgroundAgricultural activities, such as stock-farming, planting industry, and fish aquaculture, can influence the physicochemistry and biology of freshwater lakes. However, the extent to which these agricultural activities, especially those that result in eutrophication and antibiotic pollution, effect water and sediment-associated microbial ecology, remains unclear.MethodsWe performed a geospatial analysis of water and sediment associated microbial community structure, as well as physicochemical parameters and antibiotic pollution, across 18 sites in Honghu lake, which range from impacted to less-impacted by agricultural pollution. Furthermore, the co-occurrence network of water and sediment were built and compared accorded to the agricultural activities.ResultsPhysicochemical properties including TN, TP, NO3--N, and NO2--N were correlated with microbial compositional differences in water samples. Likewise, in sediment samples, Sed-OM and Sed-TN correlated with microbial diversity. Oxytetracycline and tetracycline concentration described the majority of the variance in taxonomic and predicted functional diversity between impacted and less-impacted sites in water and sediment samples, respectively. Finally, the structure of microbial co-associations was influenced by the eutrophication and antibiotic pollution.ConclusionThese analyses of the composition and structure of water and sediment microbial communities in anthropologically-impacted lakes are imperative for effective environmental pollution monitoring. Likewise, the exploration of the associations between environmental variables (e.g. physicochemical properties, and antibiotics) and community structure is important in the assessment of lake water quality and its ability to sustain agriculture. These results show agricultural practices can negatively influence not only the physicochemical properties, but also the biodiversity of microbial communities associated with the Honghu lake ecosystem. And these results provide compelling evidence that the microbial community can be used as a sentinel of eutrophication and antibiotics pollution risk associated with agricultural activity; and that proper monitoring of this environment is vital to maintain a sustainable environment in Honghu lake.

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“…Los fragmentos amplificados fueron secuenciados a través del análisis de las secuencias obtenidas en cuanto a la calidad de los nucleótidos y la similitud, utilizando el programa MEGA (TAMURA et al, 2013) versión 6 con posterior comparación con las secuencias depositadas en el GenBank (BENSON et al, 2002) utilizando el Basic Local Alignment Search Tool (BLAST) ((ALTSCHUN et al, 1997).…”

Section: Estudio De La Viabilidad Bajo Diferentes Valores De Ph (Cai Et Al1999)unclassified

“…Diferentes géneros bacterianos aislados de ambiente de carcinicultura han sido identificados y evaluados con resistencia a la tetraciclina/oxitetraciclina: Bacillus (SUNDARAMANICKAM et al, 2015) y Aeromonas-Shewanella-Vibrio-Lactococcus (YANO et al, 2011) entre otros géneros.…”

Section: Bacterias Del Génerounclassified

Evaluación De Estirpes Bacterianas Para La Formación De Consorcio Probiótico Para Uso en El Cultivo De Camarones Marinos: Litopenaeus Vannamei / Evaluation of Bacterial Strains for the Formation of a Probiotic Consortium for Use in the Cultivation of Marine Shrimps: Litopenaeus Vannamei

Rodríguez¹,

Silva²,

Abreu

et al. 2020

BJD

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El objetivo del presente trabajo fue evaluar estirpes bacterianas con características probióticas, aisladas del tracto intestinal de camarones marinos (Litopenaeus vannamei). Ciento noventa y una (191) estirpes fueron evaluadas para su selección basada en pruebas fenotípicas y genotípicas incluyendo: factores de virulencia (elastasa, gelatinasa, caseinasa, lípasa e fosfolipasa), tolerancia a diferentes temperaturas (4°C e 40°C) y pHs (5 e 9), prueba de susceptibilidad a antimicrobianos, prueba de agregación, antagonismo y prueba de identificación molecular (secuenciamiento-gen 16S rRNA). Las estirpes identificadas fueron agrupadas en diferentes géneros: Bacillus, Vibrios, Staphylococcus y un grupo con representantes de diferentes géneros bacterianos. Fueron formados tres grupos bacterianos prioritarios atendiendo a la respuesta de las estirpes frente a las diferentes pruebas analizadas. La mayoría de las estirpes (66,45%) presentó resistência frente a la oxitetraciclina. Todas las estirpes presentaron susceptibilidad frente al cloranfenicol y la tetraciclina. Ninguna estirpe presentó antagonismo frente al patógeno Vibrio parahaemolyticus. De las 70 estirpes que formaron parte de los tres grupos prioritarios, 16 estirpes (22,85%) resultaron antagónicas frente a Vibrio harvey. Doce estirpes bacterianas mostraron resultados satisfactorios en la técnica de antagonismo cruzado. Fueron formados tres consorcios bacterianos con aquellas estirpes que cumplieron los requisitos como probiótico potencial.

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Isolation and molecular characterization of multidrug-resistant halophilic bacteria from shrimp farm effluents of Parangipettai coastal waters

Cited by 23 publications

References 29 publications

Agricultural Risk Factors Influence Microbial Ecology in Honghu Lake

Agricultural Risk Factors Influence Microbial Ecology in Honghu Lake

Agricultural Pollution Risks Influence Microbial Ecology in Honghu Lake

Evaluación De Estirpes Bacterianas Para La Formación De Consorcio Probiótico Para Uso en El Cultivo De Camarones Marinos: Litopenaeus Vannamei / Evaluation of Bacterial Strains for the Formation of a Probiotic Consortium for Use in the Cultivation of Marine Shrimps: Litopenaeus Vannamei

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