Isolation and Molecular Characterization of Non-Sorbitol Fermenting Escherichia coli Isolated From Fresh Ground Beef

Maktabi, Siavash; Zarei, Mehdi; Mohammadpour, Hooriyeh

doi:10.17795/jjhs-31195

Cited by 3 publications

(2 citation statements)

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“…Fantelli and Stephan [ 24 ] and Maktabi et al . [ 25 ] reported a lower occurrence rate of 2.3% and 1.5% than the present study from minced beef samples. Next to beef kheema, beef samples represented the highest % in the occurrence of STEC.…”

Section: Discussioncontrasting

confidence: 83%

Occurrence and quantification of Shiga toxin-producing Escherichia coli from food matrices

2018

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Aim:The objective of the study was to detect Shiga toxin-producing Escherichia coli (STEC) and develop a quantitative polymerase chain reaction (qPCR) assay to quantify the bacterial DNA present in different food matrices.Materials and Methods:A total of 758 samples were collected during a period from January 2015 to December 2016 from Kozhikode, Thrissur, and Alappuzha districts of Kerala. The samples consisted of raw milk (135), pasteurized milk (100), beef (132), buffalo meat (130), chevon (104), beef kheema (115), and beef sausage (42). All the samples collected were subjected to isolation and identification of STEC by conventional culture technique. Confirmation of virulence genes was carried out using PCR. For the quantification of STEC in different food matrices, a qPCR was standardized against stx1 gene of STEC by the construction of standard curve using SYBR green chemistry.Results:The overall occurrence of STEC in raw milk (n=135), beef (n=132), buffalo meat (n=130), chevon (n=104), and beef kheema (n=115) samples collected from Kozhikode, Thrissur, and Alappuzha districts of Kerala was 19.26%, 41.6%, 16.92%, 28.85%, and 41.74%, respectively. PCR revealed the presence of stx 1 and stx 2 genes in 88.46 and 83.64 and 30.77 and 40.00% of STEC isolates from raw milk and beef samples, respectively, while 100% of the STEC isolates from buffalo beef and beef kheema samples carried stx 1 gene. Real-time qPCR assay was used to quantify the bacterial cells present in different food matrices. The standard curve was developed, and the slopes, intercept, and R2 of linear regression curves were −3.10, 34.24, and 0.99, respectively.Conclusion:The considerably high occurrence of STEC in the study confirms the importance of foods of animal origin as a vehicle of infection to humans. In the present study, on comparing the overall occurrence of STEC, the highest percentage of occurrence was reported in beef kheema samples. The study shows the need for rigid food safety measures to combat the potential pathogenic effects of harmful bacteria throughout the production chain from production to consumption.

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Section: Discussioncontrasting

confidence: 83%

Occurrence and quantification of Shiga toxin-producing Escherichia coli from food matrices

2018

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“…The prevalence of E. coli O157:H7 in beef meat samples from our study is comparable with the results of Heuvelink, Zwartkruis‐Nahuis, Beumer, and de‐Boer (), who reported a prevalence rate of 10.4% in beef from the Netherlands. The occurrence of E. coli O157:H7 in beef samples was reported as more than 8% in South Africa (Hajian, Rahimi, & Mommtaz, ), 6.8% in China, 1.5–8% in Iran (Abdul‐Raouf, Ammar, & Beuchat, ; Maktabi, Zarei, & Mohammadpour, ), 9% in raw beef in Saudi Arabia, 9% in Egypt, 8.2% in Thailand, and 3.7% in the U.S. (Hessain et al, ; Rahimi, Kazemeini, & Salajegheh, ).…”

Section: Discussionmentioning

confidence: 99%

Multiplex polymerase chain reaction detection of Shiga toxin genes and antibiotic sensitivity of Escherichia coli O157:H7 isolated from beef meat in Quetta, Pakistan

Samad

Abbas

Ahmad

et al. 2018

Journal of Food Safety

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The objective of this study was to conduct a qualitative analysis of raw beef meat sold in the city of Quetta, Pakistan for presence and drug sensitivity of the potentially pathogenic Escherichia coli strain O157:H7. The study used 200 raw beef meat samples collected from retail butcher shops. Conventional and rapid biochemical tests, latex agglutination and multiplex polymerase chain reaction (PCR) using primers designed for the rfb O157 and flic H7 genes were used to detect E. coli O157:H7. All O157:H7 isolates were also tested for Shiga toxin genes stx 1 and stx 2. The prevalence of E. coli O157:H7 in collected beef meat samples was 10%. Detection through PCR was found more sensitive than detection of O and H antigens. The quantity of E. coli O157:H7 isolates positive for Shiga toxins was 50% (20% for stx 1, 45% for stx 2 and 10% for both stx 1 and stx 2). Season wise variation showed highest E. coli O157:H7 prevalence during summer months. A further concern is that E. coli O157:H7 isolates were resistant to a range of common antibiotics. The results indicate an urgent need for applying proper food hygiene practices in the Quetta region to reduce incidence of foodborne diseases and they also emphasize the global problem of antimicrobial resistance. Practical applications E. coli O157:H7 is as a potentially threatening foodborne pathogen. A significant prevalence of E. coli O157:H7 detected in raw beef meat from retail outlets in the city of Quetta indicates an urgent need for applying proper food hygiene practices in the Quetta region to reduce the incidence of foodborne diseases. Furthermore, resistance of the E. coli O157:H7 isolates to a range of commonly used antibiotics emphasizes the global problem of antimicrobial resistance. The multiplex PCR method used here is a reliable, sensitive, and relatively rapid technique for detecting E. coli O157:H7 in food and environmental samples and important for ongoing surveillance to minimize contamination of raw meat products and associated cross contamination by E. coli O157:H7.

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Etiology of Bloody Diarrhea in Children and Prevalence of E. Coli O157:h7

Kareem¹,

Omer²

2020

JSMC

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Background Infectious diarrhea continues to be a health burden worldwide especially in children living in developing countries. The main etiologies of bloody diarrhea in developing regions are Entamoeba histolytica and bacteria such as Shigella and other bacteria. Objectives To determine the causative agents of bloody diarrhea in children and the prevalence of Enterohemorrhagic Escherichia coli in children with bloody diarrhea. Materials and Methods A cross-sectional study conducted at Dr.Jamal Ahmad Rashid Teaching Pediatric Hospital in Sulaimani city from October 2018 to August 2019. We examined stool specimens from inpatient children with diarrhea using fresh mount. We further investigated the bloody stools by cultivation on several bacteriological media. Growth was identified and the causative agents were determined based on culture, Gram stain, biochemical tests, Serological test and VITEK® 2 system. Finally, we used multiplex PCR to identify EHEC O157:H7 and Shiga toxin genes. Results From 2589 fresh mount stool examination, 117 (4.5%) were identified as bloody diarrhea based on finding RBCs. Of the 117 cultivated stool specimens, bacteria were identified as the cause of bloody diarrhea in 73(62.4%), E. histolytica/ E. dispar in 36 (30.8 %), bacteria and E.histolytica/ E. dispar in 6 (5.1%), while in 2 (1.7%) specimen, the cause remain unidentified. The isolated bacteria were Shigella spp. (56, 69%), Slamonella spp. (11, 14%), Enteroinvasive Escherichia coli (6, 7%), Campylobacter spp. (3, 4%) and Enterohemorrhagic Escherichia coli (3, 4 %). Two EHEC showed shiga toxin type two gene. Conclusion Shigella spp. and E. histolytica were the most prevalent agents of bloody diarrhea in children aged 7 months to 12 years. Enterohemorrhagic Escherichia coli harboring shiga toxin type 2 gene was identified in bloody diarrhea but in fewer cases compared to other bacteria.

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Isolation and Molecular Characterization of Non-Sorbitol Fermenting Escherichia coli Isolated From Fresh Ground Beef

Cited by 3 publications

References 28 publications

Occurrence and quantification of Shiga toxin-producing Escherichia coli from food matrices

Occurrence and quantification of Shiga toxin-producing Escherichia coli from food matrices

Multiplex polymerase chain reaction detection of Shiga toxin genes and antibiotic sensitivity of Escherichia coli O157:H7 isolated from beef meat in Quetta, Pakistan

Etiology of Bloody Diarrhea in Children and Prevalence of E. Coli O157:h7

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