Isolation and screening of lactic acid bacteria associated with the gastrointestinal tracts of abalone at various life stages for probiotic candidates

Amin, Muhamad; Adams, Mark B.; Burke, Christopher M.; Bolch, Christopher J. S.

doi:10.1016/j.aqrep.2020.100378

Cited by 21 publications

(20 citation statements)

References 39 publications

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“…Among tested isolates, LCN13 proved to have significant activity against S. typhimurium ATCC 14028 (18.3 ± 1.52 mm), E. coli ATCC 11105 (24.7 ± 2.14 mm), and MRSE ATCC 35984 (31.6 ± 2.93 mm). This result was in agreement with previous studies showing that L. plantarum and L. pentaceus had the ability to inhibit the growth of 3 tested pathogenic bacteria (Tambekar & Bhutada, 2010;Amin et al, 2020). The mode of action of LAB is attributed to H2O2 production, organic acids, and especially secondary metabolites such as bacteriocin (Hütt et al, 2006).…”

Section: Screening Of High Acid-and Antibioticproducing Lactic Acid B...supporting

confidence: 92%

“…Based on the phenotypic and molecular identification, strain LCN13 was identified as L. plantarum LCN13. Resistance to bile salts produced in the gastrointestinal tract is one of the important probiotic properties since bile severely damages lipids and fatty acids of the cell membrane, leading to cell death (Amin et al, 2020). Surprisingly, L. plantarum LCN13 could survive well in 0.8% ox-bile salt after 14 hours of incubation.…”

Section: Identification Of Lcn13 Strainmentioning

confidence: 99%

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Efficacy of biosynthesizing folate, riboflavin and typical probiotic traits reveal the potential use of \(\textit{Lactobacillus plantarum}\) LCN13 as a feed additive for swine farming

Tung

Lien

et al. 2022

AJB

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Lactic acid bacteria have been advocated as probiotics to replace antibiotic growth promoters, improve growth performance, and reduce cost production in swine farming. The aim of this study is to identify and evaluate the probiotic traits of strain LCN13 isolated from traditionally fermented feed for swine. Thirty-five bacterial isolates with different morphological characteristics were isolated, among which 9 isolates showed the ability to produce lactic acid and antibacterial activity against pathogenic bacteria. Among them, isolate LCN13 exhibited a strong capacity to produce lactic acid (18.5 ± 0.31 g/L), inhibited gastrointestinal pathogens such as Salmonella typhimurium ATCC 14028 (18.3 ± 1.52 mm), Escherichia coli ATCC 11105 (24.7 ± 2.14 mm), and Staphylococcus epidermidis ATCC 35984 (31.6 ± 2.93 mm), and produced 182 ng/mL folate and 233 ng/mL riboflavin as measured by LC-MS/MS after 24 hours of incubation. Based on morphological, biochemical, and 16S rRNA gene analysis, the isolate LCN13 was identified as Lactobacillus plantarum. Phenotypic analysis revealed that L. plantarum LCN13 showed remarkable resistance to 1.2% ox-bile salt, 2.0 mM H2O2, and pH 3.0. In addition, the ability to produce high levels of folate (253.6 ± 10.7 ng/mL) and riboflavin (312.0 ± 12.2 ng/mL) after 48 hours was exploited for the first time in the L. plantarum. Taken together, L. plantarum LCN13 might serve as a potential probiotic candidate for animal farming.

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Section: Screening Of High Acid-and Antibioticproducing Lactic Acid B...supporting

confidence: 92%

Section: Identification Of Lcn13 Strainmentioning

confidence: 99%

Efficacy of biosynthesizing folate, riboflavin and typical probiotic traits reveal the potential use of \(\textit{Lactobacillus plantarum}\) LCN13 as a feed additive for swine farming

Tung

Lien

et al. 2022

AJB

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“…In this study, all 17 isolates tested for GIT-related stress tolerance exhibited a high potential to tolerate a low pH (2), 0.3% bile salt concentration, lysozyme (100 mg/mL), and 0.4 % phenol. The ability of LAB isolates to tolerate a low pH and bile is not a surprise, as one of LAB’s natural habitat is the gastrointestinal tract of humans or animals [ 58 ]. Our results are in agreement with previous findings that reported on the survival of different LAB strains, including Enterococcus spp., L. salivarius , L. johnsonii , and L. reuteri , isolated from healthy chicken gut, being able to survive at pH 2 and 0.3% bile concentration [ 59 , 60 , 61 ].…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus reuteri and Enterococcus faecium from Poultry Gut Reduce Mucin Adhesion and Biofilm Formation of Cephalosporin and Fluoroquinolone-Resistant Salmonella enterica

Siddique

Azim

Ali

et al. 2021

Animals

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Non-typhoidal Salmonella (NTS) can cause infection in poultry, livestock, and humans. Although the use of antimicrobials as feed additives is prohibited, the previous indiscriminate use and poor regulatory oversight in some parts of the world have resulted in increased bacterial resistance to antimicrobials, including cephalosporins and fluoroquinolones, which are among the limited treatment options available against NTS. This study aimed to isolate potential probiotic lactic acid bacteria (LAB) strains from the poultry gut to inhibit fluoroquinolone and cephalosporin resistant MDR Salmonella Typhimurium and S. Enteritidis. The safety profile of the LAB isolates was evaluated for the hemolytic activity, DNase activity, and antibiotic resistance. Based on the safety results, three possible probiotic LAB candidates for in vitro Salmonella control were chosen. Candidate LAB isolates were identified by 16S rDNA sequencing as Lactobacillus reuteri PFS4, Enterococcus faecium PFS13, and Enterococcus faecium PFS14. These strains demonstrated a good tolerance to gastrointestinal-related stresses, including gastric acid, bile, lysozyme, and phenol. In addition, the isolates that were able to auto aggregate had the ability to co-aggregate with MDR S. Typhimurium and S. Enteritidis. Furthermore, LAB strains competitively reduced the adhesion of pathogens to porcine mucin Type III in co-culture studies. The probiotic combination of the selected LAB isolates inhibited the biofilm formation of S. Typhimurium FML15 and S. Enteritidis FML18 by 90% and 92%, respectively. In addition, the cell-free supernatant (CFS) of the LAB culture significantly reduced the growth of Salmonella in vitro. Thus, L. reuteri PFS4, E. faecium PFS13, and E. faecium PFS 14 are potential probiotics that could be used to control MDR S. Typhimurium and S. Enteritidis in poultry. Future investigations are required to elucidate the in vivo potential of these probiotic candidates as Salmonella control agents in poultry and animal feed.

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“…According to Salamone et al (21), several types of bacteria produced proteolytic enzymes of Vibrio sp. Enterococcus faecium MA002, Enterococcus lactis MA056, Leuconostoc mesenteroides MA064, Enterococcus lactis MA068, dan Enterococcus lactis MA084 (22). This might help the degradation process of organic material in shrimp ponds; however, it would be dangerous when Vibrio dominates the bacterial population in the pond, and hence the number must be limited (23).…”

Section: Introductionmentioning

confidence: 99%

Isolation and molecular identification of proteolytic bacteria from vaname shrimp (Lithopenaeus Vannamei) ponds as probiotic agents

Fitriadi¹,

palupi²,

Setyawan³

et al. 2023

IJVS

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Vannamei shrimp culture in intensive and traditional ponds is developing rapidly in Indonesia. The development starts with technology, high density, and feeding systems. It results in very high organic material produced in the aquaculture ponds, and this water quality management is necessarily maintained. This study aims to isolate and identify the molecular of proteolytic bacteria from Vannamei shrimp aquaculture ponds using a different system that is as probiotic agents. This study used a survey method and random sampling technique from traditional and intensive shrimp aquaculture. The results showed that the proportion of proteolytic bacteria in traditional ponds was higher than that in intensive ponds. The screening based on colony morphology and highest activity index obtained 4 bacterial isolates of Vibrio sp. and Pseudoalteromonas white an identity value of 98.23-99.80%. The value of this activity was observed at 7.0 cm, subsequently inhibiting Vibrio sp. by 1.5 cm. The bacteria were also found to develop at pH 7 and a temperature of 28°C adequately. Additionally, the maximum proteolytic activity at a 48-hours incubation indicated their potential relevance as a biotechnological probiotic. Further studies on the potential of Pseudoalteromonas ganghwensis strains found that the bacteria have antimicrobial activity and are sensitive to antibiotics that can be used as aquaculture probiotic agents.

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Isolation and screening of lactic acid bacteria associated with the gastrointestinal tracts of abalone at various life stages for probiotic candidates

Cited by 21 publications

References 39 publications

Efficacy of biosynthesizing folate, riboflavin and typical probiotic traits reveal the potential use of \(\textit{Lactobacillus plantarum}\) LCN13 as a feed additive for swine farming

Efficacy of biosynthesizing folate, riboflavin and typical probiotic traits reveal the potential use of \(\textit{Lactobacillus plantarum}\) LCN13 as a feed additive for swine farming

Lactobacillus reuteri and Enterococcus faecium from Poultry Gut Reduce Mucin Adhesion and Biofilm Formation of Cephalosporin and Fluoroquinolone-Resistant Salmonella enterica

Isolation and molecular identification of proteolytic bacteria from vaname shrimp (Lithopenaeus Vannamei) ponds as probiotic agents

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