Isolation and structural characterization of levan produced by probiotic Bacillus tequilensis-GM from Tunisian fermented goat milk

Abid, Yousra; Azabou, Samia; Casillo, Angela; Gharsallah, Houda; Jemil, Nawel; Lanzetta, Rosa; Attia, Hamadi; Corsaro, Maria Michela

doi:10.1016/j.ijbiomac.2019.04.130

Cited by 30 publications

(16 citation statements)

References 36 publications

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“…all three bacterial isolates B. subtilis CS, B. tequilensis CL, and W. cibaria p3B were susceptible to each tested antibiotic which ensures its incapability to transfer the antibiotic resistance genes as well. Previous reports supporting our study suggest that probiotic bacterial isolates B. subtilis AMS6 from Churpi(Manhar et al 2016), B. tequilensis GM from fermented goat milk(Abid et al 2019), Weissella strains from fermented cucumber…”

supporting

confidence: 88%

“…The consumption of fermented foods containing antibiotic-resistant bacteria may affect the transfer of these microorganisms to the intestine and the resistant genes might get transferred to the pathogenic bacteria sometimes too (Abid et al 2019). Hence, susceptibility against antibiotics is considered to be one of the best probiotic characteristics (Patel et al 2009).…”

Section: Discussionmentioning

confidence: 99%

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Probiotic characterization of bacterial strains from fermented South Indian tomato pickle and country chicken intestine having antioxidative and antiproliferative activities

et al. 2021

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Aim: The present study aims to evaluate the potential antioxidant and antiproliferative properties of probiotic bacterial isolates Weissella cibaria p3B, Bacillus subtilis CS, and Bacillus tequilensis CL, isolated from South Indian fermented tomato pickle (homemade) and gut content of indigenous country chicken. Methods and Results: The bacterial isolates exhibited antimicrobial activity against food-borne, human pathogenic bacteria, along with better survival under different bile and acidic conditions, hydrophobicity towards several hydrocarbons, and adherence to intestinal epithelial cells (INT-407 cells). Also, the intact cell (IC) mixture of the three species showed better DPPH, ABTS, and Fe 2+ chelating activity as compared to the individual IC or cell extract (CE) activity. Among the three bacterial species, W. cibaria p3B revealed maximum antiproliferative activity against HeLa and Caco-2 cancer cells, all of which were nontoxic to INT-407 cells. Apart from being non-hemolytic, the bacterial isolates did not display any necrotic inhibition in HeLa and Caco-2 cells. The cell free supernatant (CFS) of the three bacterial isolates were tested for the production of antimicrobial peptides or bacteriocins. It found that the CFS of bacterial isolates was stable at various temperature, pH and sensitive to proteolytic enzymes confirms protenoius in nature of the antimicrobil peptides or bacteriocins. Conclusion:The bacterial isolates showed promising antimicrobial, antioxidant as well as antiproliferative activities with better survival ability at different pH and bile concentrations. The three bacterial isolates were able to produce potential antimicrobial peptides or bacteriocins. Significance and Impact of the study: These results indicate better compatibility of our bacterial isolates against synthetic drugs to avoid adverse side effects and can be processed as dietary supplements against food and human pathogens. They can also provide antioxidative and antiproliferative benefits to humans and animals.

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supporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Probiotic characterization of bacterial strains from fermented South Indian tomato pickle and country chicken intestine having antioxidative and antiproliferative activities

et al. 2021

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“…1) and was therefore confirmed as B. aryabhattai. Though, levan synthesis has been reported in several Bacillus species (Abid et al, 2019;Belghith et al, 2012;Bersaneti, Pan, Baldo, & Celligoi, 2017;M. Li et al, 2015), we hereby report first time its production by a B. aryabhattai strain.…”

Section: Isolation and Identification Of Bacterial Strainmentioning

confidence: 77%

“…The EPS was produced by growing the isolate on GYC broth (supplemented with 20% sucrose) in an incubator at 37 °C for h. The culture was centrifuged at 6000×g for 10 min and supernatant was separated. The proteins in the polysaccharide were precipitated by adding trichloro acetic acid (TCA) at a final concentration of 14% (w/v) to the supernatant followed by shaking incubation (50 rpm at 23 °C for 40 mins) and centrifugation at 10,000 rpm for 10 min at 4 °C (Abid et al, 2019). Subsequently, EPS was purified by ethanol precipitation according to the procedure reported earlier (Anwar et al, 2010).…”

Section: Production and Purification Of Exopolysaccharidementioning

confidence: 99%

Production and characterization of a high molecular weight levan and fructooligosaccharides from a rhizospheric isolate of Bacillus aryabhattai

Nasir

Sattar

Ashfaq

et al. 2020

LWT

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Bacillus aryabhattai GYC2-3 strain, capable of synthesizing EPS, was isolated from the rhizosphere of Taraxacum spp. plant. The EPS was identified as levan by 13 C NMR spectroscopy and methylation analysis. The levan produced had a high weight average molecular weight of 5.317 × 10 7 Da and degree of branching 5.19%. Thin layer chromatography and highperformance anion-exchange chromatography revealed that it is capable of producing broad range of fructooligosaccharides (FOS) and higher levels of FOS synthesis were observed with increasing sucrose concentrations. The optimum temperature, initial pH and sucrose concentration for levan biosynthesis were studied to be 30 °C, 8.0 and 250 g/L, respectively.Levan production was increased by ~38% (26 g/L) when provided aeration as compare to static cultures. To the best of our knowledge, this is the first report on biosynthesis of levan and FOS by a Bacillus aryabhattai strain.

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“…To assess the influence of pH value on the levan production by P. agglomerans ZMR7, the production medium was adjusted at various pH values (4.0, 5.0, 6.0, 7.0 and 8.0). The optimal incubation period for levan production was investigated by the determination of levan yield after different incubation periods (24,48,72,96, and 120 h).…”

Section: Optimization Of Levan Productionmentioning

confidence: 99%

Bioactive Levan-Type Exopolysaccharide Produced by Pantoea agglomerans ZMR7: Characterization and Optimization for Enhanced Production

Al-Qaysi

Al-Haideri

AL-Shimmary

et al. 2021

J. Microbiol. Biotechnol.

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Levan is an industrially important, functional biopolymer with considerable applications in the food and pharmaceutical fields owing to its safety and biocompatibility. Here, levan-type exopolysaccharide produced by Pantoea agglomerans ZMR7 was purified by cold ethanol precipitation and characterized using TLC, FTIR, 1 H, and 13 C NMR spectroscopy. The maximum production of levan (28.4 g/l) was achieved when sucrose and ammonium chloride were used as carbon and nitrogen sources, respectively, at 35°C and an initial pH of 8.0. Some biomedical applications of levan like antitumor, antiparasitic, and antioxidant activities were investigated in vitro. The results revealed the ability of levan at different concentrations to decrease the viability of rhabdomyosarcoma and breast cancer cells compared with untreated cancer cells. Levan appeared also to have high antiparasitic activity against the promastigote of Leishmania tropica. Furthermore, levan had strong DPPH radical scavenging (antioxidant) activity. These findings suggest that levan produced by P. agglomerans ZMR7 can serve as a natural biopolymer candidate for the pharmaceutical and medical fields.

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Isolation and structural characterization of levan produced by probiotic Bacillus tequilensis-GM from Tunisian fermented goat milk

Cited by 30 publications

References 36 publications

Probiotic characterization of bacterial strains from fermented South Indian tomato pickle and country chicken intestine having antioxidative and antiproliferative activities

Probiotic characterization of bacterial strains from fermented South Indian tomato pickle and country chicken intestine having antioxidative and antiproliferative activities

Production and characterization of a high molecular weight levan and fructooligosaccharides from a rhizospheric isolate of Bacillus aryabhattai

Bioactive Levan-Type Exopolysaccharide Produced by Pantoea agglomerans ZMR7: Characterization and Optimization for Enhanced Production

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