“…A possible explanation may be that microorganisms need an adaptation period to produce a considerable population size with the secretion of necessary degradative enzymes to cause noticeable degradation. This may be the reason for the prolonged lag phase when grown in a medium with a low density of cells in degradation of chlorpyrifos [42]. Fig.…”
Section: Biodegradation Of Quinalphos By Bacillus Thuringiensismentioning
confidence: 99%
“…500 and 1000 mg L −1 , to the extent of 90% after 2 weeks. The prolonged lag phase in the degradation of chlorpyrifos at higher concentration could be due to the time taken for the adaptation of microorganisms to produce the necessary enzymes [42]. …”
Section: Biodegradation Of Quinalphos By Bacillus Thuringiensismentioning
BackgroundThe extensive and intensive uses of organophosphorus insecticide—quinalphos in agriculture, pose a health hazard to animals, humans, and environment because of its persistence in the soil and crops. However, there is no much information available on the biodegradation of quinalphos by the soil micro-organisms, which play a significant role in detoxifying pesticides in the environment; so research is initiated in biodegradation of quinalphos.ResultsA soil bacterium strain, capable of utilizing quinalphos as its sole source of carbon and energy, was isolated from soil via the enrichment method on minimal salts medium (MSM). On the basis of morphological, biochemical and 16S rRNA gene sequence analysis, the bacterium was identified as to be Bacillus thuringiensis. Bacillus thuringiensis grew on quinalphos with a generation time of 28.38 min or 0.473 h in logarithmic phase. Maximum degradation of quinalphos was observed with an inoculum of 1.0 OD, an optimum pH (6.5–7.5), and an optimum temperature of 35–37 °C. Among the additional carbon and nitrogen sources, the carbon source—sodium acetate and nitrogen source—a yeast extract marginally improved the rate of degradation of quinalphos.ConclusionsDisplay of degradation of quinalphos by B. thuringiensis in liquid culture in the present study indicates the potential of the culture for decontamination of quinalphos in polluted environment sites.
“…A possible explanation may be that microorganisms need an adaptation period to produce a considerable population size with the secretion of necessary degradative enzymes to cause noticeable degradation. This may be the reason for the prolonged lag phase when grown in a medium with a low density of cells in degradation of chlorpyrifos [42]. Fig.…”
Section: Biodegradation Of Quinalphos By Bacillus Thuringiensismentioning
confidence: 99%
“…500 and 1000 mg L −1 , to the extent of 90% after 2 weeks. The prolonged lag phase in the degradation of chlorpyrifos at higher concentration could be due to the time taken for the adaptation of microorganisms to produce the necessary enzymes [42]. …”
Section: Biodegradation Of Quinalphos By Bacillus Thuringiensismentioning
BackgroundThe extensive and intensive uses of organophosphorus insecticide—quinalphos in agriculture, pose a health hazard to animals, humans, and environment because of its persistence in the soil and crops. However, there is no much information available on the biodegradation of quinalphos by the soil micro-organisms, which play a significant role in detoxifying pesticides in the environment; so research is initiated in biodegradation of quinalphos.ResultsA soil bacterium strain, capable of utilizing quinalphos as its sole source of carbon and energy, was isolated from soil via the enrichment method on minimal salts medium (MSM). On the basis of morphological, biochemical and 16S rRNA gene sequence analysis, the bacterium was identified as to be Bacillus thuringiensis. Bacillus thuringiensis grew on quinalphos with a generation time of 28.38 min or 0.473 h in logarithmic phase. Maximum degradation of quinalphos was observed with an inoculum of 1.0 OD, an optimum pH (6.5–7.5), and an optimum temperature of 35–37 °C. Among the additional carbon and nitrogen sources, the carbon source—sodium acetate and nitrogen source—a yeast extract marginally improved the rate of degradation of quinalphos.ConclusionsDisplay of degradation of quinalphos by B. thuringiensis in liquid culture in the present study indicates the potential of the culture for decontamination of quinalphos in polluted environment sites.
A large scale electro-bioremediation test is carried out in a prototype of 32 m 3. Soil polluted with a mixture of two herbicides 2,4-D and oxyfluorfen undergoes a combined treatment in which the electrochemically assisted remediation produced by six electrodes placed in an hexagonal alternating configuration is aimed to be enhanced with the addition of concentrated activated sludge in a central position of the prototype. Results demonstrate that this technology can remove pollutants down to a very low concentration although, as compared to a previous study, its performance does not differ significantly of that followed in a process in which bioremediation was not included. In both studies, volatilization was found to be the key mechanisms to explain the removal of both herbicides. After a treatment period slightly over month, only 7.10 % of the 2,4-D and 9.22% of the oxyfluorfen remained in soil and these values are very similar to those obtained by applying non biological EKF in which 10.10 of the 2,4-D and 13.03% of the oxyfluorfen remained in the soil after the treatment. Results pointed out that it is very difficult to optimize simultaneously electrokinetic and biological processes because most of the processes caused by the electric field affect negatively to the microorganisms viability. Results also pointed out the significance of the sampling procedure to characterize an electro-bioremediation process at full scale.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
