Isolation, Enumeration, and Maintenance of Rumen Anaerobic Fungi in Roll Tubes

Joblin, K. N.

doi:10.1128/aem.42.6.1119-1122.1981

Cited by 224 publications

(104 citation statements)

References 7 publications

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“…The counts obtained for the anaerobic fungi reflect the number of zoospores in the cultures, not the number of vegetative fungal thalli which are immobilized on the substrate. The zoospore counts are in good accord with the number of zoospores in rumen contents (Orpin 1975;Joblin 1981).…”

Section: Resultssupporting

confidence: 66%

“…Javefaciens strain 007 and Bacteroides succinogenes Bac 22 were from the culture collection of the Rowett Institute. Cellulolytic rumen fungi were isolated from roughage fed sheep by the method of Joblin (1981). Strain PNK 2 was described by Joblin (1981) and on the basis of its multiflagellate zoospores and general morphology is assigned to the species Neocallimastix frontalis (Orpin 1975).…”

Section: Bacterial a N D F U N G A L C U L T U R E Smentioning

confidence: 99%

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The use of tritiated cellulose for the rapid enumeration of cellulolytic anaerobes

Stewart¹,

Duncan²,

Joblin

1985

Lett Appl Microbiol

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Section: Resultssupporting

confidence: 66%

Section: Bacterial a N D F U N G A L C U L T U R E Smentioning

confidence: 99%

The use of tritiated cellulose for the rapid enumeration of cellulolytic anaerobes

Stewart¹,

Duncan²,

Joblin

1985

Lett Appl Microbiol

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“…Biohydrogenation by rumen fungi proceeded more slowly, however, and needed around 24 h of incubation for completion. The slower rate of biohydrogenation by rumen fungi might relate to the fungal life cycle, which lasts approximately 24 h (Joblin 1981;Heath et al 1983). In the current study, LA solution was added to fungal culture after one complete life cycle (after 24 h of preincubation).…”

Section: Discussionmentioning

confidence: 99%

Biohydrogenation of linoleic acid by rumen fungi compared with rumen bacteria

Nam

Garnsworthy

2007

J Appl Microbiol

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Aims: To investigate biohydrogenation of linoleic acid by rumen fungi compared with rumen bacteria, and to identify the fungus with the fastest biohydrogenation rate. Methods and Results: Biohydrogenation of linoleic acid by mixed rumen fungi and mixed rumen bacteria were compared in vitro. With mixed rumen bacteria, all biohydrogenation reactions were finished within 100 min of incubation and the end product of biohydrogenation was stearic acid. With mixed rumen fungi, biohydrogenation proceeded more slowly over a 24‐h period. Conjugated linoleic acid (CLA; cis‐9, trans‐11 C18 : 2) was an intermediate product, and vaccenic acid (VA; trans‐11 C18 : 1) was the end product of biohydrogenation. Fourteen pure fungal isolates were tested for biohydrogenation rate. DNA sequencing showed that the isolate with the fastest rate belonged to the Orpinomyces genus. Conclusions: It is concluded that rumen fungi have the ability to biohydrogenate linoleic acid, but biohydrogenation is slower in rumen fungi than in rumen bacteria. The end product of fungal biohydrogenation is VA, as for group A rumen bacteria. Orpinomyces is the most active biohydrogenating fungus. Significance and Impact of the Study: This is the first study to demonstrate that rumen fungi can biohydrogenate fatty acids. Fungi could influence CLA content of ruminant products.

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“…Orpinomyces joyonii A, was isolated in this laboratory from the rumen fluid of a camel. The methods used for the preparation of prereduced media and isolation techniques were essentially those of Joblin (1981). Megasphaera elsdenii L, was isolated in this laboratory from rumen fluid of a sheep on PY medium with lactate.…”

Section: Mlcro-organismsmentioning

confidence: 99%

Interaction of the rumen fungus Orpinomyces joyonii with Megasphaera elsdenii and Eubacterium limosum

Hodrová

Kopečný

Petr

1995

Lett Appl Microbiol

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The degradation and fermentation of microcrystalline cellulose were studied in monoculture of the polycentric anaerobic fungus Orpinomyces joyonii and in co-cultures with the rumen bacteria Megasphaera elsdenii and Eubacterium limosum. More than 25% of cellulose hydrolysis products (glucose and cellodextrins) were released by the fungus into the medium after 8 d of cultivation. These products were metabolized by bacteria in mixed cultures. In co-culture with the fungus M. elsdenii and E. limosum increased the extent of microcrystalline cellulose degradation by 10.12% and 7.96%, respectively. Biomass yield in co-cultures was increased by 89.9% and 59.4% for M. elsdenii and E. limosum. Y cellulose for fungus alone was 52.29 g dry matter mol-1 glucose. These values were 64.93 and 55.92 g mol-1 glucose unit in co-culture with M. elsdenii and E. limosum, respectively.

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Isolation, Enumeration, and Maintenance of Rumen Anaerobic Fungi in Roll Tubes

Abstract: Anaerobic phycomycetous fungi have been isolated from rumen fluid by using the Hungate roll tube technique. Cultures on solid substrates in roll tubes can be stored for long periods, and fungal zoospores can be enumerated in roll tubes.

Cited by 224 publications

References 7 publications

The use of tritiated cellulose for the rapid enumeration of cellulolytic anaerobes

The use of tritiated cellulose for the rapid enumeration of cellulolytic anaerobes

Biohydrogenation of linoleic acid by rumen fungi compared with rumen bacteria

Interaction of the rumen fungus Orpinomyces joyonii with Megasphaera elsdenii and Eubacterium limosum

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