Isolation, Enumeration, and Preservation of theVibrionaceae

Pre-Enrichment of Estuarine and Fresh Water Environmental Samples with Sodium Chloride Yields in Better Recovery of <i>Vibrio parahaemolyticus</i>

“…APW is the preferred enrichment medium for vibrios including V. parahaemolyticus. The high pH of the medium (pH close to 9) and NaCl concentration inhibit many other bacteria and favor vibrios [19]. The selective enrichment of the marine or estuarine environmental samples with APW has yielded in better recovery of V.…”

Simultaneous detection of Pathogenic Vibrio species using multiplex real-time PCR

“…APW, initially developed for the cultivation of V. cholerae (Donovan & van Netten, 1995), can be used for the cultivation of other Vibrio species including our target species (Gomez-Gil & Roque, 2006) and is a widely used enrichment medium recommended by the United States Food and Drug Administration (FDA) (Food and Drug Administration, 2010). The use of non-selective enrichment medium might reduce the final sensitivity of the detection method since microorganisms other than the target species can grow in the medium, thus increasing the competing background microorganisms in the culture (Kamio, Hara-Kudo, Miyasaka, Yahiro, & Konuma, 2008).…”

“…Since our primary goal was to detect three pathogenic Vibrio species (V. cholerae, V. parahaemolyticus, and V. vulnificus) in a single reaction, we used alkaline peptone water (APW) for enrichment broth instead of species-specific selective enrichment broth such as TTPW, GSTP, SCB, SPB, and PNCC (Gomez-Gil & Roque, 2006). APW, initially developed for the cultivation of V. cholerae (Donovan & van Netten, 1995), can be used for the cultivation of other Vibrio species including our target species (Gomez-Gil & Roque, 2006) and is a widely used enrichment medium recommended by the United States Food and Drug Administration (FDA) (Food and Drug Administration, 2010).…”

Differential specificity of selective culture media for enumeration of pathogenic vibrios: Advantages and limitations of multi-plating methods

“…Although pathogenic vibrio species are the primary target of these media, the growth of other vibrios and even non-vibrio species has been reported (Gomez-Gil and Roque, 2006). Thiosulfate-citrate-bile salts-sucrose (TCBS) is primarily used to culture pathogenic vibrio strains and it is the most widely used medium for vibrio isolation (Gomez-Gil and Roque, 2006).…”