Isolation, identification, and in vivo evaluation of the novel antihypertensive peptide, VSKRLNGDA, derived from chicken blood cells

Wongngam, Wasana; Roytrakul, Sittiruk; Mitani, Takahiko; Katayama, Shigeru; Nakamura, Soichiro; Yongsawatdigul, Jirawat

doi:10.1016/j.procbio.2022.02.011

Cited by 9 publications

(9 citation statements)

References 39 publications

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“…Several studies have been focused on the valorization of chicken blood or its components, including corpuscle and plasma fractions or isolated red blood cells, through the generation of ACEi peptides [ 66 , 84 , 85 , 86 , 87 ] ( Table 2 ). Results from these studies indicated that ACEi activity of the blood protein hydrolysates was deeply influenced by hydrolysis conditions and protein substrate [ 66 , 84 ].…”

Section: Resultsmentioning

confidence: 99%

“…In this regard, it has been observed that the presence of hydrophobic residues such as Trp, Tyr, Pro, or Phe at the C-terminal end, as well as aliphatic amino acids such as Gly, Val, Leu, and Ile at the N-terminal position, leads to higher ACE inhibitions [ 130 , 131 , 132 ]. Specifically, ACEi peptides have been identified in hydrolysates from feet, blood corpuscle, legs with claws, bone, viscera, or a mixture of combs and wattles, showing IC 50 values lower than 100 µM in many cases [ 22 , 85 , 97 , 98 , 133 ]. The protein source of these peptides depends on the used byproduct.…”

Section: Resultsmentioning

confidence: 99%

“…The protein source of these peptides depends on the used byproduct. For example, in combs and wattles, the main sources of ACEi peptides were collagen and elastin [ 98 ], while, in blood corpuscle, the main sources were cytochrome Bc1, hemoglobin, and fibrinogen, among others [ 85 ]. In addition, antihypertensive effects of peptides isolated from feet, bone, and blood corpuscle hydrolysates have also been evidenced in SHR [ 22 , 97 , 101 , 134 ].…”

Section: Resultsmentioning

confidence: 99%

“…Lastly, VSKRLNGDA is another peptide that has been widely studied to elucidate the mechanisms involved in its antihypertensive effects. This peptide, identified in a blood corpuscle hydrolysate, reduced both SBP and DBP in SHR after its acute administration at 12.5, 25, and 50 mg/kg BW [ 85 ]. Interestingly, at 12 h post administration, the dose of 50 mg/kg BW continued exerting its antihypertensive effect, especially noted in SBP (41.8 mmHg).…”

Section: Resultsmentioning

confidence: 99%

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Valorization of Chicken Slaughterhouse Byproducts to Obtain Antihypertensive Peptides

et al. 2023

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Hypertension (HTN) is the leading cause of premature deaths worldwide and the main preventable risk factor for cardiovascular diseases. Therefore, there is a current need for new therapeutics to manage this condition. In this regard, protein hydrolysates containing antihypertensive bioactive peptides are of increasing interest. Thus, agri-food industry byproducts have emerged as a valuable source to obtain these hydrolysates as they are rich in proteins and inexpensive. Among these, byproducts from animal origin stand out as they are abundantly generated worldwide. Hence, this review is focused on evaluating the potential role of chicken slaughterhouse byproducts as a source of peptides for managing HTN. Several of these byproducts such as blood, bones, skins, and especially, chicken feet have been used to obtain protein hydrolysates with angiotensin-converting enzyme (ACE)-inhibitory activity and blood pressure-lowering effects. An increase in levels of endogenous antioxidant compounds, a reduction in ACE activity, and an improvement of HTN-associated endothelial dysfunction were the mechanisms underlying their effects. However, most of these studies were carried out in animal models, and further clinical studies are needed in order to confirm these antihypertensive properties. This would increase the value of these byproducts, contributing to the circular economy model of slaughterhouses.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Valorization of Chicken Slaughterhouse Byproducts to Obtain Antihypertensive Peptides

et al. 2023

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“…Chicken is an important source of peptides with various beneficial bioactivities, including antioxidation, anti-pancreatic lipase activity, anti-hypertension, alcohol dehydrogenase stabilization, immune boosting, insulin resistance amelioration, and learning enhancement [ 4 , 16 , 17 , 18 , 19 , 20 , 21 ]. Moreover, the bioactive agents derived from poultry industrial products or co-products will provide advantages such as unlimited and manageable supplies, a bio-circular and green economy, and industry sustainability.…”

Section: Introductionmentioning

confidence: 99%

Anserine/Carnosine-Rich Extract from Thai Native Chicken Suppresses Melanogenesis via Activation of ERK Signaling Pathway

et al. 2022

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Skin hyperpigmentation is an aesthetic problem that leads to psychosocial issues. Thus, skin whitening agents from agro- and poultry-industrial co-products are considered high economic value ingredients of interest for sustainable application. Therefore, this study aimed to determine the cosmeceutical potential of anserine/carnosine-rich chicken extract (ACCE) from the Thai native chicken Pradu Hang Dam Mor Kor 55 (PD) meat. The chemical composition was identified and quantified using the HPLC-UV method. Then, the antioxidation potential of the extract was compared to that of L-anserine and L-carnosine, using 1,1-diphenyl-2-picrylhydrazyl assay and shikonin-induced production of reactive oxygen species in CCD-986Sk cell models, and the anti-melanogenesis effect in the MNT-1 melanoma cell line model was investigated. Furthermore, related mechanisms were identified using colorimetric tyrosinase assay and the Western blot technique. The ACCE was composed of L-anserine and L-carnosine as two major constituents. In a dose-dependent manner, ACCE, L-anserine, and L-carnosine manifested significant antioxidation potential and significant reduction of melanin production. Activation of the extracellular signal-regulated kinase (ERK) signaling pathway and inhibition of tyrosinase activity of ACCE were demonstrated as the mechanisms of the anti-melanogenesis effect. In conclusion, ACCE has been revealed as a potential cosmeceutical agent due to its antioxidation and anti-melanogenic activity in association with L-anserine and L-carnosine composition and biomolecular regulating ability. Therefore, further studies and development should be considered to support the utilization of anserine/carnosine-rich chicken extract in the cosmetic industry for economic value creation and sustainability.

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Long-term intake of corn gluten meal protein hydrolysate attenuated hypertension development and modulated associated plasma metabolite levels in spontaneously hypertensive rats

Chanajon,

Girgih,

Oluwagunwa

et al. 2024

Journal of Functional Foods

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Isolation, identification, and in vivo evaluation of the novel antihypertensive peptide, VSKRLNGDA, derived from chicken blood cells

Cited by 9 publications

References 39 publications

Valorization of Chicken Slaughterhouse Byproducts to Obtain Antihypertensive Peptides

Valorization of Chicken Slaughterhouse Byproducts to Obtain Antihypertensive Peptides

Anserine/Carnosine-Rich Extract from Thai Native Chicken Suppresses Melanogenesis via Activation of ERK Signaling Pathway

Long-term intake of corn gluten meal protein hydrolysate attenuated hypertension development and modulated associated plasma metabolite levels in spontaneously hypertensive rats

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