Isolation of 55 microsatellite markers for Jatropha curcas and its closely related species

Phumichai, Chalermpol; Phumichai, Thitaporn; Kongsiri, Nongluck; Wongkaew, Arunee; Sripichit, Prapa; Kaveeta, R.

doi:10.1007/s10535-011-0061-3

Cited by 15 publications

(9 citation statements)

References 9 publications

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“…Development of codominant markers and the construction of an integrated linkage map from four F 2 mapping populations For the construction of linkage maps, codominant markers such as single nucleotide polymorphisms (SNP) and simple sequence repeats (SSR) are the most useful as they provide information on both alleles present in a diploid species such as J. curcas. At the commencement of this study, very few codominant markers were available for J. curcas and these were mainly in the form of SSR (Basha et al, 2009;Phumichai et al, 2011). To provide sufficient markers for the construction of a linkage map, additional SNP markers were obtained from genomic DNA using the CRoPS â technique (File S1).…”

Section: Resultsmentioning

confidence: 99%

Linkage mapping in the oilseed crop Jatropha curcas L. reveals a locus controlling the biosynthesis of phorbol esters which cause seed toxicity

King

Montes²,

Clarke

et al. 2013

Plant Biotechnology Journal

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Current efforts to grow the tropical oilseed crop Jatropha curcas L. economically are hampered by the lack of cultivars and the presence of toxic phorbol esters (PE) within the seeds of most provenances. These PE restrict the conversion of seed cake into animal feed, although naturally occurring ‘nontoxic’ provenances exist which produce seed lacking PE. As an important step towards the development of genetically improved varieties of J. curcas, we constructed a linkage map from four F2 mapping populations. The consensus linkage map contains 502 codominant markers, distributed over 11 linkage groups, with a mean marker density of 1.8 cM per unique locus. Analysis of the inheritance of PE biosynthesis indicated that this is a maternally controlled dominant monogenic trait. This maternal control is due to biosynthesis of the PE occurring only within maternal tissues. The trait segregated 3 : 1 within seeds collected from F2 plants, and QTL analysis revealed that a locus on linkage group 8 was responsible for phorbol ester biosynthesis. By taking advantage of the draft genome assemblies of J. curcas and Ricinus communis (castor), a comparative mapping approach was used to develop additional markers to fine map this mutation within 2.3 cM. The linkage map provides a framework for the dissection of agronomic traits in J. curcas, and the development of improved varieties by marker-assisted breeding. The identification of the locus responsible for PE biosynthesis means that it is now possible to rapidly breed new nontoxic varieties.

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Section: Resultsmentioning

confidence: 99%

Linkage mapping in the oilseed crop Jatropha curcas L. reveals a locus controlling the biosynthesis of phorbol esters which cause seed toxicity

King

Montes²,

Clarke

et al. 2013

Plant Biotechnology Journal

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“…Five SSR markers (JCT15, JCT16, JCT17, JCT27, and JCT81) (Phumichai et al 2011) and five new SSR markers developed using a modified biotin-streptavidin capture method (Phumichai et al 2011) were used in this study (Table 2). PCR reactions were prepared in 10 µL reaction volumes containing approximately 10 ng of template DNA, 50 mM KCl, 20 mM Tris-Cl buffer (pH 8.0), 1.5 mM MgCl2, 0.2 µM of each primer, 0.4 mM of each dNTP, and 0.5 unit of Taq DNA polymerase (Fermentas).…”

Section: Dna Extraction and Ssr Analysismentioning

confidence: 99%

Genetic diversity of physic nut (Jatropha curcas L.) revealed by SSR markers

Na-ek

Wongkaew

Phumichai³

et al. 2011

J. Crop Sci. Biotechnol.

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Ten microsatellite markers were used to investigate genetic diversity and genetic structure among 32 accessions of Jatropha curcas. Low levels of average genetic diversity were observed (HE = 0.160). A dendrogram produced by the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) based on Nei's genetic distances revealed 3 groups among 32 accessions. The genetic differentiation (FST) among two groups was significant (P < 0.01). The model-based Bayesian clustering method indicated that a population structure (∆K) was separated into two groups. The analysis of molecular variance (AMOVA) showed higher variability (63.753%) among groups than within groups (36.247%). These findings could assist in defining the best method of genetic conservation and studies in breeding programs for genetic improvement of J. curcas.

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“…For the genotyping, 10 markers previously shown to be polymorphic for J. curcas (Pamidimarri et al, 2009;Achten et al, 2010;Phumichai et al, 2011) were used. A detailed description of the primers is presented in Table 2.…”

Section: Genotyping With Microsatellite Markersmentioning

confidence: 99%

Population structure of jatropha and its implication for the breeding program

Santos¹,

Ferreira²,

Pasqual³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Jatropha (Jatropha curcas L.) has potential as an oilseed crop that requires the development of technology for its exploitation. The objective of this study was to assess the population structure and the genetic diversity in jatropha accessions at a global level using simple sequence repeat (SSR) molecular markers. Jatropha accessions (N = 109) from 10 countries were genotyped using 10 SSR markers. The results showed a low level of genetic diversity among 92 accessions originating from India,

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Isolation of 55 microsatellite markers for Jatropha curcas and its closely related species

Cited by 15 publications

References 9 publications

Linkage mapping in the oilseed crop Jatropha curcas L. reveals a locus controlling the biosynthesis of phorbol esters which cause seed toxicity

Linkage mapping in the oilseed crop Jatropha curcas L. reveals a locus controlling the biosynthesis of phorbol esters which cause seed toxicity

Genetic diversity of physic nut (Jatropha curcas L.) revealed by SSR markers

Population structure of jatropha and its implication for the breeding program

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