Isolation of a cDNA clone encoding a KATP channel-like protein expressed in insulin-secreting cells, localization of the human gene to chromosome band 21q22.1, and linkage studies with NIDDM

Tsaur, M. L.; Menzel, S.; Lai, F. P.; Espinosa, R.; Concannon, P.; Spielman, R. S.; Hanis, C. L.; Cox, N. J.; Beau, M. M. Le; German, M. S.; al, et

doi:10.2337/diabetes.44.5.592

Cited by 15 publications

(20 citation statements)

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“…Four YAC clones were identified in the screen mapping to human chromosome 3. Two of four of the candidate YACs were confirmed by PCR as containing the PPAR␥ sequence (Tsaur et al 1995). The contig formed by the two PPAR␥-gene-containing YACs is as follows: 754 D 7 and 897 A 6.…”

Section: Ppar␥mentioning

confidence: 99%

“…The RH screen placed APOJ at 3.46 cR from STS WI-1172 which is 111.17 cR from the top of human chromosome 8. Seven YACs were selected from the contig WC 8.4 of the Whitehead anchored map for human chromosome 8, and 4 of 7 of the candidate YACs contained APOJ (Tsaur et al 1995). The APOJ gene containing YAC contig overlaps in a centromeric-to-telomeric manner as follows: 755 B 3, 771 G 9, 791 B 9, and 810 D 6.…”

Section: Apojmentioning

confidence: 99%

“…The RH screen placed ART at 2.94 cR from STS WI-5594, which is 299.70 cR from the top of human chromosome 16. Six YACs were selected from the contigs WC16.5, 16.6, and 16.7 of the Whitehead anchored map for human chromosome 16, and 5 of 6 of the candidate YACs were confirmed by PCR as containing the ART sequence (Tsaur et al 1995). These YACs 767 C 6, 782 G 9, 837 F 9, 877 C 6, and 898 G 5 are part of contigs WC16.5, 16.6, and 16.7 of the Whitehead anchored map and are localized to the 16q21-22.1 region of chromosome 16 (Hudson et al 1995).…”

Section: Artmentioning

confidence: 99%

“…Candidate-gene-positive YACs were confirmed by PCR as containing the candidate gene of interest (Tsaur et al 1995). YAC contigs were aligned for the various candidate genes relative to the known map positions of the microsatellites contained within the contigs as elucidated from microsatellite mapping.…”

mentioning

confidence: 99%

“…The PCR was carried out in a GeneAmp 9600 PCR system (ABI), and PCR products were analyzed on 2% ‫ן1‬ TAE agarose gels. DNA for CEPH mega-YACs was isolated as reported (Tsaur et al 1995). Assays for STS content of mega-YAC clones were conducted in 25-ml PCR reactions as above.…”

mentioning

confidence: 99%

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Microsatellite Marker Content Mapping of 12 Candidate Genes for Obesity: Assembly of Seven Obesity Screening Panels for Automated Genotyping

Winick

Friedman²

1998

Genome Res.

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Twin studies, adoption studies, and studies of familial aggregation indicate that obesity has a genetic component. Whereas, the genetic factors predisposing to obesity have been elucidated for several rare syndromes, the factors responsible for obesity in the general population have remained elusive. Genetic studies of complex traits are often accelerated by the use of candidate genes. To facilitate genetic studies of human obesity, seven multiplex panels of candidate genes for obesity that are suitable for fluorescent genotyping have been assembled. The multiplex panels are composed of 66 microsatellite markers linked tightly to 16 human gene products that are of potential importance in the control of body weight or linked to syndromic forms of obesity. As part of these efforts 12 previously cloned genes have been placed on the human physical map. In addition the chromosomal location of three of these genes, ART,NYP Y6R, and PPARγ, are reported for the first time. These resources will be of use in studies to identify the genetic factors responsible for human obesity. [Figures are available at http://www.genome.org.]

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Section: Ppar␥mentioning

confidence: 99%

Section: Apojmentioning

confidence: 99%