Isolation of a novel dehydrin gene from Codonopsis lanceolata and analysis of its response to abiotic stresses

Pulla, Rama Krishna; Kim, Yujin; Kim, Myung Kyum; Senthil, Kalaiselvi; In, Jun-Gyo; Yang, Deok-Chun

doi:10.5483/bmbrep.2008.41.4.338

Cited by 18 publications

(10 citation statements)

References 42 publications

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“…There are also a poly-proline cluster located at residues 171-176 between the K1-and K2-segments and a lysine motif consisting of six residues (KKKKKK) at residues 103-108, which is speculated to function as a nuclear targeting signal sequence (NTS) (8). A search for transmembrane helices using TMHMMv2.0 did not detect any transmembrane helix in Podhn, suggesting it functions in the cytosol or nucleus rather than in membranes (9).…”

Section: Resultsmentioning

confidence: 99%

Differential expression of a poplar SK₂-type dehydrin gene in response to various stresses

Bae

Lee²,

Lee³

et al. 2009

BMB Reports

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Dehydrins are group II, late embryogenesis abundant proteins that act putatively as chaperones in stressed plants. To elucidate the function of dehydrins in poplar, we isolated the SK2-type dehydrin gene Podhn from Populus alba × P. tremula var. glandulosa suspension cells and analyzed its expression following treatments of abiotic stress, wounding and plant growth regulator. Sequence homology and phylogenetic analyses indicate Podhn encodes an acidic dehydrin (pI 5.14, 277 amino acids, predicted size 25.6 kDa) containing two lysine-rich "K-segments" and a 7-serine residue "S-segment", both characteristic of SK2-type dehydrins. Southern blots show Podhn genes form a small gene family in poplar. Podhn was expressed in all tissues examined under unstressed conditions, but most strongly in cell suspensions (especially in the stationary phase). Drought, salt, cold and exogenous abscisic acid (ABA) treatments enhanced Podhn expression, while wounding and jasmonic acid caused its reduction. Therefore, Podhn might be involved in ABA or stress response. [BMB reports 2009; 42(7): 439-443]

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Section: Resultsmentioning

confidence: 99%

Differential expression of a poplar SK₂-type dehydrin gene in response to various stresses

Bae

Lee²,

Lee³

et al. 2009

BMB Reports

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“…Furthermore, the dried roots of C. lanceolata are often used as a type of "recovery pill" to aid those who are weakened by disease, surgery or other medical therapy, blood loss or other injury, or childbirth (Fratkin et al, 1974). The value of C. lanceolata can also be revealed by numerous studies of certain specific genes, such as fructose-1,6-bisphosphate aldolase and dehydrin, or chemical products, such as saponins, involved in regulation of environmental stress and its enthnopharmacological roles (Pulla et al, 2008). Nonetheless, the underlying mechanisms that account for C. lanceolata's ethnopharmacology, however, remain largely unexplained and require further investigation.…”

Section: Introductionmentioning

confidence: 98%

Inhibitory effect of saponin fraction from Codonopsis lanceolata on immune cell-mediated inflammatory responses

Byeon

Choi²,

Hong

et al. 2009

Arch. Pharm. Res.

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Saponin components are known to be pharmaceutically, cosmetically and nutraceutically valuable principles found in various herbal medicine. In this study, we evaluated the inhibitory role of saponin fraction (SF), prepared from C. lanceolata, an ethnopharmacologically famous plant, on various inflammatory responses managed by monocytes, macrophages, lymphocytes and mast cells. SF clearly suppressed the release of nitric oxide (NO) and tumor necrosis factor (TNF)-alpha, but not prostaglandin E(2) (PGE(2)). While this fraction did not scavenge the reactivity of SNP-induced radicals in RAW264. 7 cells, it negatively modulated the phagocytic uptake of macrophages treated with FITC-dextran. Interestingly, SF completely diminished cell-cell adhesion events induced by both CD29 and CD43, but not cell-fibronectin adhesion. Concanavalin (Con) A [as well phytohemaglutinin A (PHA)]-induced proliferation of splenic lymphocytes as well as interferon (IFN)-gamma production were also clearly suppressed by SF treatment. Finally, SF also significantly blocked the degranulation process of mast cell line RBL-2H3 cell as assessed by DNP-BSA-induced beta-hexosaminidase activity. The anti-inflammatory activities of SF on NO production seemed to be due to inhibition of nuclear factor (NF)-kappaB activation signaling, since it blocked the phosphorylation of inhibitor of kappaB (IkappaB)alpha as well as inducible NO synthase (iNOS) expression. Therefore, these results suggest that SF may be considered as a promising herbal medicine with potent anti-inflammatory actions.

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“…It has been demonstrated that accumulation of ClDhn mRNA is significantly induced in response to various abiotic stresses such as salt, wounding, chilling and light, at different time points within 4-48 hrs post-treatment (14). However, orthologous genes from different species sometimes exhibit different expression patterns.…”

Section: Introductionmentioning

confidence: 99%

Expression of dehydration responsive element-binding protein-3 (DREB3) under different abiotic stresses in tomato

Islam¹,

Wang²

2009

BMB Reports

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We investigated the expression pattern of dehydration responsive element-binding protein-3 in tomato under different abiotic stresses. Full length LeDREB3 cDNA was isolated from tomato plant, followed by phylogenetic analysis based on deduced amino acid sequences that revealed significant sequence similarity to DREB proteins belonging to diverse families of plant species. Southern blot analysis showed duplicate copies of LeDREB3 in the tomato genome while organ-specific expression profiling indicated constitutive expression of LeDREB3 in all tested organs, which was particularly strong in flower. LeDREB3 expression was significantly induced by Nacl, drought, low temperature and H2O2. Moreover,

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Isolation of a novel dehydrin gene from Codonopsis lanceolata and analysis of its response to abiotic stresses

Cited by 18 publications

References 42 publications

Differential expression of a poplar SK₂-type dehydrin gene in response to various stresses

Differential expression of a poplar SK₂-type dehydrin gene in response to various stresses

Inhibitory effect of saponin fraction from Codonopsis lanceolata on immune cell-mediated inflammatory responses

Expression of dehydration responsive element-binding protein-3 (DREB3) under different abiotic stresses in tomato

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Isolation of a novel dehydrin gene from Codonopsis lanceolata and analysis of its response to abiotic stresses

Cited by 18 publications

References 42 publications

Differential expression of a poplar SK2-type dehydrin gene in response to various stresses

Differential expression of a poplar SK2-type dehydrin gene in response to various stresses

Inhibitory effect of saponin fraction from Codonopsis lanceolata on immune cell-mediated inflammatory responses

Expression of dehydration responsive element-binding protein-3 (DREB3) under different abiotic stresses in tomato

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Differential expression of a poplar SK₂-type dehydrin gene in response to various stresses

Differential expression of a poplar SK₂-type dehydrin gene in response to various stresses