Isolation of a selected microbial consortium from a pesticide-contaminated mix-load site soil capable of degrading the herbicides atrazine and alachlor

PesticideLambda-Cyhalothrin DegradationUsingMesorhizobium sp.(S1b) andBartonella sp.(S2b)StrainsIsolated from Cotton Crop

“…It is believed that one gram of soil have approximately more than one hundred million bacteria and 5000-7000 different bacterial strains [31]. The different microbes have been isolated from polluted soils having ability to degrade pesticides [32][33]. However the isolation of pesticide-degrading bacteria by using enrichment technique is more preferred over simple isolation techniques and this revealed a positive association between isolation of LCdegrading microbes vs. enrichment technique in MSM [34].…”

Biodegradation of Aged Residues of Atrazine and Alachlor in a Mix-Load Site Soil by Fungal Enzymes

“…After sufficient growth was achieved in the PBR, the influent was switched to a nitrogen-limiting media to induce ligninolytic activity, and the overflow effluent was monitored for LiP and MnP production. [1]. The following three treatments were evaluated: (1) 5 mL of the collected enzyme solution, (2) 5 mL of the collected enzyme solution and 1 mL 5 mM H 2 O 2 , and (3) 5 mL of sterilized distilled deionized water (DDW) and 1 mL 5 mM H 2 O 2 as the control.…”

“…Aerobic microcosms were prepared in standard 250 mL biometer flasks (Bellco, Glass Inc.,Vineland, NJ) consisting of a main body with a side-arm trap containing 0.2 N NaOH prepared with CO 2 -free DDW. Fifty grams of air-dried, ground soil collected from a 100-year-old mix-load site located in Reading, Pa [1] were placed into the main body of the biometer flasks. Site 5A soil was chosen for this study because of the lack of AT and AL degraders [1] and the presence of high concentrations AT and AL ( Table 2).…”

“…Fifty grams of air-dried, ground soil collected from a 100-year-old mix-load site located in Reading, Pa [1] were placed into the main body of the biometer flasks. Site 5A soil was chosen for this study because of the lack of AT and AL degraders [1] and the presence of high concentrations AT and AL ( Table 2). The flasks were autoclaved at 120…”

“…Bulk pesticide mixing and loading (mix-load) sites are major contributors of pesticide contamination of ground and surface waters [1]. Many of these sites contain extremely high concentrations of pesticides, fertilizers, and other organic compounds used in pesticide formulations, which may limit bioremediation efforts due to the toxicity effects of the high pesticide concentrations to the indigenous microbes [2,3], the complexity of the mixture of compounds present [4], and the natural heterogeneity of the soil and water environment [5].…”