Isolation of an Adenovirus and an Adeno-Associated Virus from Goat Kids with Enteritis

Abstract

Abstract.A dairy goat operation in Minnesota experienced a sudden, markedly increased mortality among its neonatal goats. Approximately 60 of 130 kids (46%) died. The animals had diarrhea and dyspnea of 1-2 days duration before death. Necropsy of 4 goat kids revealed marked, acute, catarrhal enteritis and fibrinous pleuropneumonia. Mannheimia haemolytica was isolated from the lungs. Basophilic inclusion bodies filling the entire nucleus were present in enterocytes of the ileum of 3 goats. Adenoviral particles were detected in the feces by electron microscopy and adenovirus was subsequently isolated from the intestinal content together with a parvo-like virus (dependovirus). Morphology, physicochemical characteristics, and neutralization tests indicated that the adenovirus resembled ovine adenovirus-2 (OAdV-2). However, the PstI restriction endonuclease pattern produced by the goat adenovirus was distinct from that of OAdV-2. This is the first report of enteritis in goats with an adenovirus antigenically related to OAdV-2 and with a parvo-like dependovirus.Four goat kids from a caprine dairy farm were submitted to the Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota, for necropsy in March 2001. The farm had 850 milking does (Nubians, LaMancha, and Bohr crosses), but the approximately 100 preparturient does and kids were kept physically separate from the milking herd. Bred does on this farm are routinely transferred to a second farm until parturition. The 4 kids had acute watery, yellow diarrhea, and all died within 4-5 hours after onset of diarrhea. Approximately 130 goat kids were born on the premises. Sixty of these kids died with similar clinical signs over a 2.5-month period during the kidding season from January to April, 2001. Only suckling bottle-fed kids were affected, with an age range of 10-40 days and an average age of 27 days. There was no sex or breed predilection. All kids were routinely separated at birth from the dam and housed initially in groups of 3-4 animals. At 30 days of age, the pens were commingled into larger groups of up to 20 kids per pen for weaning. Young stock were fed heat-pasteurized goat colostrum for 2 days, followed by cow's milk fed free choice in LamBar-type feeding units until weaning.A postmortem examination was performed on 4 kids. For histology, samples of small and large intestines, lungs, liver, kidney, spleen, brain, and heart were collected. Sections cut at 4 m were stained with he-

Novel Caprine Adeno-Associated Virus (AAV) Capsid (AAV-Go.1) Is Closely Related to the Primate AAV-5 and Has Unique Tropism and Neutralization Properties

“…Four adenovirus preparations derived from a pool of postmortem ileum samples from four young goats concurrently afflicted with viral catarrhal enteritis and bacterial fibrinous pleuropneumonia (29) were received from the National Animal Disease Center (Ames, IA) for AAV screening. The four adenovirus preparations shared the same inoculum but were propagated using different host cells (ovine turbinate cells, primary lamb kidney cells, or Madin-Darby bovine kidney cells).…”

AAV Hybrid Serotypes: Improved Vectors for Gene Delivery

“…In addition to human and primates, AAV has also been isolated from different species including cow [Schmidt, M et al, 2004], chicken [Bossis. I and Chiorini, JA, 2003], sheep [Clarke, JK et al, 1979], snake [Farkas, SL et al, 2004], lizard [Jacobson, ER et al, 1996], and goat [Olson, EJ et al, 2004]. The isolation of new AAV serotypes from animal tissues and the development of these new variants into gene therapy vectors are discussed in a separate review in this issue [Gao et al].…”

Isolation and evaluation of novel adeno-associated virus sequences from porcine tissues

“…AAV has been isolated from many organisms including human, nonhuman primates, ovine, avian, snake, bovine, murine and caprine. [1][2][3][4][5][6][7][8][9][10][11][12][13][14] It has been reported that various AAV serotypes have differing tropisms and can transduce specific organs more efficiently than others, such as the high transduction efficiency of AAV5 in airway epithelial, muscle and retina cells as compared to AAV2. 15,16 Gene transfer vectors derived from AAV can deliver genes in a variety of tissues in vivo.…”

“…24,25 In the past, AAV has conventionally been isolated from contaminated adenoviral stocks or young, sick animals or children. [1][2][3][4][5][6][7]9,12 Recently, isolation of new AAV isolates was extensively performed using PCR amplification of AAV sequences from genomic DNA of different animal species. 8,10,11,13 In this study, novel porcine AAV sequences were identified by PCR using genome walking strategies.…”