Isolation of an insertion sequence (IS1051) from Xanthomonas campestris pv. dieffenbachiae with potential use for strain identification and characterization

Berthier, Y.; Thierry, Dominique; Lemattre, M.; Guesdon, Jean‐Luc

doi:10.1128/aem.60.1.377-384.1994

Cited by 20 publications

(3 citation statements)

References 23 publications

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“…rRNA genes have highly conserved sequences, and their potential usefulness in the identification and phylogenetic studies of bacteria has been demonstrated (2,9). Specific sequences from genomic or plasmid DNA, such as repetitive elements and insertion sequences, provided useful probes for the assessment of genetic diversity and also allowed a better understanding of the pathogen population structure (1,4,8,18,28,29). Both ribotyping and RFLP analysis with the use of different DNA probes facilitated the study of the population structure of X campestris pv.…”

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confidence: 99%

Pathological and Molecular Characterization of Xanthomonas campestris Strains Causing Diseases of Cassava ( Manihot esculenta )

Verdier¹,

Boher²,

Maraite

et al. 1994

Appl Environ Microbiol

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Fifty-one strains representing Xanthomonas campestris pv. manihotis and cassavae and different pathovars occurring on plants of the family Euphorbiaceae were characterized by ribotyping with a 16S+23S rRNA probe of Escherichia coli and by restriction fragment length polymorphism analysis with a plasmid probe from X. campestris pv. manihotis. Pathogenicity tests were performed on cassava (Manihot escuknta). Histological comparative studies were conducted on strains of two pathovars of X. campestris (vascular and mesophyllic) that attack cassava. Our results indicated that X. campestris pv. manihotis and cassavae have different modes of action in the host and supplemented the taxonomic data on restriction fragment length polymorphism that

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confidence: 99%

Pathological and Molecular Characterization of Xanthomonas campestris Strains Causing Diseases of Cassava ( Manihot esculenta )

Verdier¹,

Boher²,

Maraite

et al. 1994

Appl Environ Microbiol

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“…Xanthomonas axonopodis pv. dieffenbachiae is the aetiological agent of bacterial blight of aroids (BBA), first described in the USA on Dieffenbachia maculata in 1939 aroid species is related to geographic origin and host plant (Chase et al, 1992;Berthier et al, 1994;Khoodoo & Jaufeerally-Fakim, 2004). Two phylogenetically distinct groups of strains pathogenic to aroids have been described.…”

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confidence: 99%

Comparative and collaborative studies for the validation of a nested PCR for the detection of Xanthomonas axonopodis pv. dieffenbachiae from Anthurium samples

et al. 2013

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Efficient control of Xanthomonas axonopodis pv. dieffenbachiae, the causal agent of anthurium bacterial blight, requires sensitive and reliable diagnostic tools. The European standard EN ISO 16140:2003 has been followed to compare a nested PCR assay (N-PCR) to a reference method (isolation and serological identification of bacterial colonies) and to other alternative serological detection methods. The evaluation was performed in two steps: a comparative study and a collaborative study involving 15 European laboratories. Although inclusivity was maximal (100%) for all methods, a maximal exclusivity was obtained only with N-PCR followed by an enzymatic restriction digestion of the amplicons. Exclusivity indices of 90Á6, 88Á7 and 47Á2% were found for indirect ELISA, immunofluorescence and double antibody sandwich ELISA, respectively. An exclusivity of 92Á5% was obtained with the reference method, further increased to 100% if pathogenicity tests were performed as a supplemental assay. The best level of sensitivity (relative detection level) was obtained with the reference method followed by the N-PCR assay. The N-PCR performance in terms of relative accuracy, accordance and concordance was very similar to that of the reference method. Moreover, N-PCR had undeniable advantages compared to the reference method (less labour-intensive and less time-consuming). In addition, post-test probabilities of infection were calculated to select the most appropriate detection scheme related to the prevalence of the pathogen. The N-PCR assay has since been included in a revised version of the EPPO detection protocol.

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“…Several types of DNA probes have been used for RFLP analysis of bacterial species. Avirulence (avr) genes (1,18), hrp genes (4,13,33,36), and repetitive sequences that either are of unknown nature (7,8,10,13) or are transposable elements (1,3,12,18) have been useful for studies on the population structure within pathovars, for comparing different pathovars, and for differentiating nonpathogenic and pathogenic Xanthomonas strains. Depending on the probe used, variability was observed at different levels.…”

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confidence: 99%

Genomic Variability of the Xanthomonas Pathovar mangiferaeindicae, Agent of Mango Bacterial Black Spot

Gagnevin¹,

Leach²,

Pruvost³

1997

Appl Environ Microbiol

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The genetic diversity of 138 strains of the Xanthomonas pathovar mangiferaeindicae, which were isolated from three different hosts (mango, ambarella, and pepper tree) in 14 different countries, was assessed with restriction fragment length polymorphism markers. An analysis of patterns obtained by hybridization with an hrp cluster probe from Xanthomonas oryzae pv. oryzae separated 11 of the strains from all of the other strains, which suggested that these 11 strains may not be Xanthomonas pv. mangiferaeindicae strains. Hybridization with an avirulence gene from X. oryzae pv. oryzae and a repetitive DNA fragment from Xanthomonas pv. mangiferaeindicae separated the remaining 127 strains into four groups that were consistent with both geographic and host origins. The group with the greatest diversity consisted of strains from Southeast Asia, where mango originated. Other groups and subgroups contained strains that were either from widely separated countries, which suggested that wide dissemination from a single site occurred, or from localized areas, which suggested that evolution of separate lineages of strains occurred. One group of strains contained only strains isolated from pepper trees in Réunion, indicating that pepper tree may not be an alternate host for Xanthomonas pv. mangiferaeindicae strains.

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Isolation of an insertion sequence (IS1051) from Xanthomonas campestris pv. dieffenbachiae with potential use for strain identification and characterization

Cited by 20 publications

References 23 publications

Pathological and Molecular Characterization of Xanthomonas campestris Strains Causing Diseases of Cassava ( Manihot esculenta )

Pathological and Molecular Characterization of Xanthomonas campestris Strains Causing Diseases of Cassava ( Manihot esculenta )

Comparative and collaborative studies for the validation of a nested PCR for the detection of Xanthomonas axonopodis pv. dieffenbachiae from Anthurium samples

Genomic Variability of the Xanthomonas Pathovar mangiferaeindicae, Agent of Mango Bacterial Black Spot

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