Pathological and Molecular Characterization of
            <i>Xanthomonas campestris</i>
            Strains Causing Diseases of Cassava (
            <i>Manihot esculenta</i>
            )

Verdier, Valérie; Boher, Bernard; Maraite, Henri; Geiger, Jean-Paul

doi:10.1128/aem.60.12.4478-4486.1994

Cited by 43 publications

(23 citation statements)

References 26 publications

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“…Probes. Membranes were hybridized with a plasmid probe, designated as pthB (previously named pF3), and two repetitive DNA elements, pBS6 and pBS8, previously characterized (40). Probe pthB is a 5.4-kb EcoRI fragment that was cloned from an indigenous plasmid and harbors a pathogenicity gene related to the avr/pth gene family (39).…”

Section: Methodsmentioning

confidence: 99%

“…manihotis strains (40). Results revealed that the African strains are homogeneous, whereas in South America the pathogen is highly diverse (40). This is to be expected, because the Manihot genus originates from South and Central America, where the centers of diversity are in Brazil and Mexico (29).…”

mentioning

confidence: 97%

“…This technique is precise and discriminatory in differentiating among X. axonopodis pv. manihotis strains (40). Results revealed that the African strains are homogeneous, whereas in South America the pathogen is highly diverse (40).…”

mentioning

confidence: 98%

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Geographical Differentiation of the Population of Xanthomonas axonopodis pv. manihotis in Colombia

Restrepo¹,

Verdier²

1997

Appl Environ Microbiol

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Analyses of DNA polymorphism and virulence variation were used to evaluate the population structure of Xanthomonas axonopodis pv. manihotis, the pathogen causing cassava bacterial blight in Colombia. We collected strains from the major cassava-growing regions which can be grouped into different edaphoclimatic zones (ECZs) according to environmental conditions, production constraints, and economic parameters. DNA polymorphism was assessed by a restriction fragment length polymorphism analysis, using an X. axonopodis pv. manihotis plasmid DNA sequence (pthB) as a probe to evaluate the genetic relatedness among 189 Colombian strains. The sampling intensity permitted the estimation of genetic differentiation within and among ECZs, sites, and fields and even within an individual plant. A multiple correspondence analysis indicated that the Colombian X. axonopodis pv. manihotis population showed a high degree of diversity relative to X. axonopodis pv. manihotis populations studied previously, and the entire collection was grouped into seven clusters. A general correlation was observed between the clusters and the geographical origin of the strains, as each cluster was largely composed of strains from the same ECZ. Representative strains, identified with pthB, were further characterized by ribotyping, hybridization to two repetitive genomic probes (pBS6 and pBS8), and restriction analysis of plasmid contents to evaluate the complementarity of these markers. Virulence variation was observed within the Colombian collection. Strains of different aggressiveness were found in all ecological zones, but no correlation between virulence variation and DNA polymorphism was observed. The genetic and virulence analyses contribute to understanding the X. axonopodis pv. manihotis population structure in Colombia.

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Section: Methodsmentioning

confidence: 99%

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confidence: 97%

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Geographical Differentiation of the Population of Xanthomonas axonopodis pv. manihotis in Colombia

Restrepo¹,

Verdier²

1997

Appl Environ Microbiol

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“…Fifty‐two isolates collected in Colombia from different locations were grouped into four groups of virulence after inoculation onto three cultivars (Anonymous, 1987). Isolates also showed differences in the speed of symptom development, suggesting variation in aggressiveness (Verdier et al ., 1993, 1994). More recently, 10 pathotypes were defined among 91 Xam isolates in Venezuela, using five cassava cultivars as differentials (Verdier et al ., 1998).…”

Section: Introductionmentioning

confidence: 99%

Characterization of pathotypes among isolates of Xanthomonas axonopodis pv. manihotis in Colombia

Restrepo

Duque

Verdier³

2000

Plant Pathology

112

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Cassava bacterial blight, caused by Xanthomonas axonopodis pv. manihotis (Xam) is a destructive disease occurring in most cassava growing-areas. Although Colombian isolates of Xam differ in DNA polymorphism and pathogenicity, no suitable host differentials have been identified to demonstrate physiological specialization. A set of 26 Xam isolates from three edaphoclimatic zones (ECZs) in Colombia was selected for inoculation on a set of 17 potential cassava differentials. Leaf inoculation and stem puncture were used in order to detect possible specific interactions between cultivars and isolates. Cultivar £ isolate interaction was highly significant (P , 0´001) after stem inoculation, but not after leaf inoculation. The stem inoculation technique was selected as a method for resistance screening of cassava cultivars for bacterial blight resistance. A highly significant interaction was also detected when cultivar behaviour was rated as area under the disease progress curve (AUDPC) after stem inoculation. Different pathotypes were defined among the 26 isolates and differential cultivars were proposed to define the pathotypic composition of Xam populations in three ECZs in Colombia. The results should help to improve selection of sources of resistance to cassava bacterial blight.

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“…Strains of Xam have shown di¡erences in the speed of symptom development, suggesting variation in aggressive-ness [4^6]. Although some nonpathogenic Xam strains have been characterized genetically and phenotypically, little is known about the epidemiological importance of such strains in CBB disease [5,7]. When comparing a pathogenic Xam strain with a nonpathogenic derivative strain, an 8-kb deletion was localized on plasmid DNA of the nonpathogenic strain (V.V., personal communication).…”

Section: Introductionmentioning

confidence: 99%

Characterization of pathogenic and nonpathogenic strains of Xanthomonas axonopodis pv. manihotis by PCR-based DNA fingerprinting techniques

Gonzalez¹

2002

FEMS Microbiology Letters

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Strains of Xanthomonas axonopodis pv. manihotis (Xam) were characterized for pathogenicity and for DNA polymorphism using different PCR-based techniques. Using amplified restriction fragment length polymorphism (AFLP), strains were distinguished from each other and also from other Xanthomonas strains. Cluster analysis showed a high correlation between DNA polymorphism and pathogenicity. Four Xam strains were further analyzed using three PCR-based techniques, AFLP, AFLP^pthB and RAPD^pthB. Various primer combinations were used including primers specific to a Xam pathogenicity gene (pthB) along with RAPD or AFLP primers. The AFLP primer combinations EcoRI+T/MseI+A and EcoRI+T/MseI+T were the most efficient to discriminate among pathogenic and nonpathogenic Xam strains. Polymorphic bands were excised from the gel, amplified and cloned. Sequences analysis showed significant homology with bacterial pathogenicity island, genes involved in pathogenic fitness and regulators of virulence. Three cloned AFLP fragments were used as probes in DNA blot experiments and two of them showed significant polymorphism.

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Pathological and Molecular Characterization of Xanthomonas campestris Strains Causing Diseases of Cassava ( Manihot esculenta )

Cited by 43 publications

References 26 publications

Geographical Differentiation of the Population of Xanthomonas axonopodis pv. manihotis in Colombia

Geographical Differentiation of the Population of Xanthomonas axonopodis pv. manihotis in Colombia

Characterization of pathotypes among isolates of Xanthomonas axonopodis pv. manihotis in Colombia

Characterization of pathogenic and nonpathogenic strains of Xanthomonas axonopodis pv. manihotis by PCR-based DNA fingerprinting techniques

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