Isolation of DNA from Plants with Large Amounts of Secondary Metabolites

Friar, Elizabeth A.

doi:10.1016/s0076-6879(05)95001-5

Cited by 47 publications

(46 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Artemisia produces numerous phenolic and terpenoid compounds (Brown et al 1975;Jassbi et al 2010). Secondary compounds such as these can contaminate DNA and interfere with DNA extraction (Friar 2005). Thus, the difficulty in extracting DNA from these samples, given the high concentration in sagebrush samples, was most likely a result of secondary compounds present in the roots that were also exuded into the soil.…”

Section: Discussionmentioning

confidence: 99%

Arbuscular mycorrhizal fungal community differs between a coexisting native shrub and introduced annual grass

et al. 2012

View full text Add to dashboard Cite

Arbuscular mycorrhizal fungi (AMF) have been implicated in non-native plant invasion success and persistence. However, few studies have identified the AMF species associating directly with plant invaders, or how these associations differ from those of native plant species. Identifying changes to the AMF community due to plant invasion could yield key plant-AMF interactions necessary for the restoration of native plant communities. This research compared AMF associating with coexisting Bromus tectorum, an invasive annual grass, and Artemisia tridentata, the dominant native shrub in western North America. At three sites, soil and root samples from Bromus and Artemisia were collected. Sporulation was induced using trap cultures, and spores were identified using morphological characteristics. DNA was extracted from root and soil subsamples and amplified. Sequences obtained were aligned and analyzed to compare diversity, composition, and phylogenetic distance between hosts and sites. Richness of AMF species associated with Artemisia in cultures was higher than AMF species associated with Bromus. Gamma diversity was similar and beta diversity was higher in AMF associated with Bromus compared to Artemisia. AMF community composition differed between hosts in both cultures and roots. Two AMF species (Archaeospora trappei and Viscospora viscosum) associated more frequently with Artemisia than Bromus across multiple sites. AMF communities in Bromus roots were more phylogenetically dispersed than in Artemisia roots, indicating a greater competition for resources within the invasive grass. Bromus associated with an AMF community that differed from Artemisia in a number of ways, and these changes could restrict native plant establishment.

show abstract

Section: Discussionmentioning

confidence: 99%

Arbuscular mycorrhizal fungal community differs between a coexisting native shrub and introduced annual grass

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Both substances help with the removal of high levels of polyphenols during DNA extraction. These substances may have a synergistic effect by binding to the polyphenols (Friar, 2005). The presence of contaminants depends on the type of vegetable tissue and its state of development at the time of extraction.…”

Section: Resultsmentioning

confidence: 99%

“…Such polyphenolic compounds have been reported as contaminating agents that present obstacles to DNA purification in other plant species, including the medicinal plants Sclerocarya birrea Hochst., Aloe polyphylla Schönland ex Pillans and Barleria greenii M. Balkwill & K. Balkwill (Moyo et al, 2008), and tropical and coniferous trees (Bashalkhanov and Rajora, 2008). According to Friar (2005), the presence of polyphenols in DNA solutions inhibits the activity of enzymes that are used in molecular biology experiments, such as DNA ligases, polymerases and endonucleases, and therefore affects analyses that require the use of PCR.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simple and robust DNA extraction method for the large-scale analysis of genotypes containing high polyphenolic content, such as landracesof Solanum tuberosum and Zea mays

Barra

Salazar

Beltrán³

et al. 2012

Cienc. Inv. Agr.

View full text Add to dashboard Cite

M. Barra, E. Salazar, M. Beltrán, and B. Sagredo. 2012. Simple and robust DNA extraction method for the large-scale analysis of genotypes containing high polyphenolic content, such as landraces of Solanum tuberosum and Zea mays. Cien Inv. Agr. 39(3): 593-601. The extraction of high-quality DNA is essential for studies conducted at the molecular level in species with an abundance of contaminants in their tissues, such as some landraces of potato and maize, in which it is difficult to extract good-quality genomic DNA. Compounds such as polyphenols interfere with the amplification of DNA during polymerase chain reaction (PCR), which makes it difficult to conduct PCR-based studies of molecular markers. This article describes a simple and robust protocol for DNA isolation that was applied to potato and maize landraces and resulted in a high DNA concentration with excellent purity and quality. This method is based on the method of Krizman et al. (2006) with some modifications and was tested on potato leaves and young maize leaves. The protocol was compared with one commercial DNA extraction kit and three methods that were previously described for plant DNA extraction: Keb-Llanes et al. (2002), for plants with high polyphenolic content; Fulton et al. (1995), which was developed for Solanaceae and other herbaceous plants; and the original Krizman et al. (2006) cetyltrimethylammonium bromide (CTAB)-activated charcoal method . The method proposed is suitable for isolating DNA from potato and maize genotypes with high polyphenol contents, yielding high-quality DNA that can be utilized for molecular techniques that involve PCR, cutting with restriction enzymes and other applications. The method is short, similar to a commercial kit, but cheaper. The amount of DNA extracted from maize using our method is superior compared with other published methods tested in this article.

show abstract

“…Also, additional purification steps, requiring differential precipitation or extraction, increase the likelihood of DNA loss. Thus, protocols that can successfully remove contaminants from genomic DNA in as few steps as possible are most desirable (Friar 2005).…”

Section: Discussionmentioning

confidence: 99%

The role of adaptive evolution of phenotypic plasticity and historical population genetic processes in purple loosestrife (Lythrum salicaria L.) invasion in North America

Chun

View full text Add to dashboard Cite

Isolation of DNA from Plants with Large Amounts of Secondary Metabolites

Cited by 47 publications

References 7 publications

Arbuscular mycorrhizal fungal community differs between a coexisting native shrub and introduced annual grass

Arbuscular mycorrhizal fungal community differs between a coexisting native shrub and introduced annual grass

Simple and robust DNA extraction method for the large-scale analysis of genotypes containing high polyphenolic content, such as landracesof Solanum tuberosum and Zea mays

The role of adaptive evolution of phenotypic plasticity and historical population genetic processes in purple loosestrife (Lythrum salicaria L.) invasion in North America

Contact Info

Product

Resources

About