Isolation of fucoxanthin from edible brown algae by microwave‐assisted extraction coupled with high‐speed countercurrent chromatography

Abstract

A rapid and efficient method for the separation and purification of fucoxanthin from edible brown algae by microwave-assisted extraction coupled with high-speed countercurrent chromatography was developed. The algae were first extracted using microwave-assisted extraction, then the dried extract was dissolved and directly introduced into the high-speed countercurrent chromatography system with a two-phase solvent system consisting of hexane-ethyl acetate-ethanol-water (5:5:6:4, v/v/v/v). The isolation was done in less than 75 min, and a total of 0.83 mg, 1.09 mg, and 0.20 mg fucoxanthin were obtained from 25.0 g fresh Laminaria japonica Aresch, 1.5 g dry Undaria pinnatifida (Harv) Sur, and 15.0 g dry Sargassum fusiforme (Harv) Setch, respectively. The purity of fucoxanthin determined by HPLC was over 90% and its structure was further identified by LC-ESI-MS and (1) H-NMR.

Fucoxanthin induces growth arrest and apoptosis in human bladder cancer T24 cells by up-regulation of p21 and down-regulation of mortalin

“…It has been reported to induce apoptosis in several human cancer cell lines such as PC-3 [12], colon cancer Caco-2 [13], and leukemia HL-60 cells [14] through down-regulating Bcl-2 expression. In addition, fucoxanthin was found to inhibit the growth of tumor cells (hepatic carcinoma HepG2, colon adenocarcinoma WiDr, prostate cancer DU145 and leukemia cancer HL-60 cells), induce cell cycle arrest at the G 1 phase by cyclin D, p21WAF1/Cip1, and MAPK regulation, and inactivate the Bcl-xl pathway by the generation of reactive oxygen species (ROS), respectively [15][16][17]. Our previous research indicated that fucoxanthin can induce the apoptosis of human bladder cancer EJ-1 cells [18], which was identified to be the same cell line as T24 cells [19].…”

Purification and Formulation of Xanthophyll for Pharmaceutical Use: Current Strategies and Future Trends

“…In this technology, parameters such as the selection of the two-phase solvent system, the elution mode, flow rate, and rotation speed are manipulated in order to obtain higher purification of the product of interest [54]. Some studies have reported the use of HSCCC technology for astaxanthin [39], fucoxanthin [38], lutein and zeaxanthin purification [40,41] (Tab. 2).…”

“…HSCCC technology is another type of liquid-liquid partition chromatography based on hydrodynamic principles [38] that is widely used in the preparative isolation of natural products. This technique is preferred due to its larger separation capacity than HPLC and the possibility to process crude extracts [41].…”

Comparison of extraction methods for selected carotenoids from macroalgae and the assessment of their seasonal/spatial variation

“…SCO 2 provided the highest purity of Fucoxanthin (0.151 mg FE/mg extract) and also the highest purity of Xanthophyll (0.008 mg XE/mg extract). Xiao et al, (2012) isolated fucoxanthin from edible brown algae by microwave assisted extraction coupled with high speed countercurrent chromatography and investigated the purity of each fucoxanthin fraction. They found the…”