Isolation of infectious pancreatic necrosis virus, Ab serotype, from an epizootic in farmed turbot, Scophthalmus maximus

Castric, J.; Baudin-Laurencin, F.; Coustans, Marie-Françoise; Auffret, Marc

doi:10.1016/0044-8486(87)90016-0

Cited by 28 publications

(17 citation statements)

References 10 publications

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“…This is well associated with the necrosis of the haematopoietic tissue of kidney previously reported (Gastric et al, 1987;Novoa et al, 1993a). However, Mortensen et al (1993) could not find this lesion in experimentally infected turbot.…”

Section: Discussionmentioning

confidence: 69%

“…To date, there are few reports about the course of viral infections in turbot and only Gastric et al (1987) reported the presence of neutralizing antibodies against a viral pathogen.…”

Section: Introductionmentioning

confidence: 99%

“…Most of the juveniles that survive a birnavirus infection and the adults develop a chronic infection and they become asymptomatic carriers which can spread the virus via faeces, urine and sexual products (Yamamoto, 1975;Reno et al, 1978;Wolf, 1988). Although in recent years the virus has been isolated from non-salmonid fish (fresh water and marine species) (Wolf, 1988), it is only since 1987 that isolation of birnaviruses has been made from cultured turbot (Scophthalmus maximus) in France (Gastric et al, 1987), Spain (Ledo et al, 1990;Novoa et al, 1993a,b) and Norway (Mortensen et al, 1990). The presence of birnavirus in turbot did not always result in mortality or clinical disease as Roberts (1978) pointed out for salmonids.…”

Section: Introductionmentioning

confidence: 99%

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Entry and sequential distribution of an aquatic birnavirus in turbot (Scophthalmus maximus)

1995

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Experimental infections with a birnavims strain isolated from turbot (Scophthalmus maximus) were conducted by immersion and intraperitoneal injection in turbot. Virus could be recovered in skin, mucus and intestine from fish infected by immersion, only up to 3 days post-infection. The distribution in internal organs was fast after intraperitoneal injection and virus was recovered with high titres from intestine, kidney, liver and spleen. The lowest titres were detected in brain and gills. Virus was isolated from plasma and blood fractions of the intraperitoneally infected turbot. Preliminary work on the influence on infection of turbot by intraperitoneal injection on the haematological parameters shows that there is no significant difference between infected and uninfected fish in the haematocrit value, number of leukocytes and concentration of plasma proteins.

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Section: Discussionmentioning

confidence: 69%

“…To date, there are few reports about the course of viral infections in turbot and only Gastric et al (1987) reported the presence of neutralizing antibodies against a viral pathogen.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Entry and sequential distribution of an aquatic birnavirus in turbot (Scophthalmus maximus)

1995

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“…Scophthalmus maximus include Herpesvirus scophthalmi (Buchanan & Madely 1978), infectious pancreatic necrosis virus (IPNV) (Castric et al 1987), and a virus suggested to be a member of the aquareovirus group (Lupiani et al 1989). More recently viral haemorrhagic septicaemia virus (VHSV), a lyssavirus belonging to the rhabdoviridae, was found in marketsized farmed turbot (Schlotfeldt et al 1991) and a picornavirus-like agent causing encephalomyelitis among turbot fry (Bloch et al 1991) has been described.…”

mentioning

confidence: 99%

An indovirus-like agent associated with systemic infection in cultured turbot Scophlhalmus maximus fry in Denmark

Bloch¹,

Larsen²

1993

Dis. Aquat. Org.

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A disease in a Danish turbot Scophthalmus maximus rearing unit caused the death of approximately 50 000 fry corresponding to a mortality of 70 %. The initial signs of disease were reduced feed intake, lethargy and darkening in pigmentation, especially of the tail and fins. Later there was atypical swimming, e.g. swimming on edge, and spasms in the terminal stages. An iridovirus-like agent, measuring ca 170 nm in diameter (160 to 185 nm), was detected by electron microscopy in samples of fin, gill, liver, ludney, spleen, heart, pancreas, intestinal collagen, and in 1 of 3 brain samples examined. After decontamination of the facilities, no new outbreaks have occurred.

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“…The virus was isolated for the first time in 1957 (Wolf 1988). It is widespread in the aquatic environment and causes disease in several fish species (McKnight & Roberts 1976, Sano 1976, Ahne 1980, Sorimachi & Hara 1985, Castric et al 1987, Biering et al 1994). …”

Section: Introductionmentioning

confidence: 99%

Induction of infectious pancreatic necrosis (IPN) in Atlantic salmon Salmo salar and brook trout Salvelinus fontinalis by bath challenge of fry with infectious pancreatic necrosis virus (IPNV) serotype Sp

Taksdal¹,

Stangeland²,

Bh³

1997

Dis. Aquat. Org.

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ABSTRACT. Thirteen week old Atlantic salmon Salmo salar fry and 15 wk old brook trout Salvelinus fontinalis with average weights of 0.19 and 0.17 g, respectively, were bath challenged with infectious pancreatic necrosis virus (IPNV), serotype Sp, NI strain. The virus had been isolated from a clinical outbreak of infectious pancreatic necrosis (IPN) in Atlantic salmon parr. Cell culture supernatant after a first passage of virus was used as the infective material. Approximately 400 fry of each species were divided into 2 parallel tanks with challenged fish and 2 parallel tanks with non-challenged fish. At 69 d after challenge, cumulative mortalities of Atlantic salmon were 76 and 82 % for the challenged groups and 6 and 15% for the non-challenged groups. The corresponding values for brook trout were 42 and 4 3 % for the challenged groups and 8 and 10% for the non-challenged groups. The onset of mortality in challenged Atlantic salmon was delayed compared with the onset in brook trout. IPNV was reisolated from the fry of all challenged groups. Histological examination of sampled moribund fish showed hepatic degeneration or necrosis and exocrine pancreatic necrosis in fry from all challenged groups. The presence of IPNV in the lesions was demonstrated immunohistochemically. Virological, histological and immunohistochemical examinations of fry sampled prior to challenge and of non-challenged fry gave negative results. This study represents, to our knowledge, the first report of an experimental induction of clinical IPN in Atlantic salmon fry.

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Isolation of infectious pancreatic necrosis virus, Ab serotype, from an epizootic in farmed turbot, Scophthalmus maximus

Cited by 28 publications

References 10 publications

Entry and sequential distribution of an aquatic birnavirus in turbot (Scophthalmus maximus)

Entry and sequential distribution of an aquatic birnavirus in turbot (Scophthalmus maximus)

An indovirus-like agent associated with systemic infection in cultured turbot Scophlhalmus maximus fry in Denmark

Induction of infectious pancreatic necrosis (IPN) in Atlantic salmon Salmo salar and brook trout Salvelinus fontinalis by bath challenge of fry with infectious pancreatic necrosis virus (IPNV) serotype Sp

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